Colony replicating device

• US 4,717,667 A
• Filed: 07/11/1984
• Issued: 01/05/1988
• Est. Priority Date: 07/11/1984
• Status: Expired due to Fees

• Current Assignee: FMC Corporation
• Original Assignee: FMC Corporation
• Inventors: Provonchee, Richard B.
• Primary Examiner(s): Warden, Robert J.
• Assistant Examiner(s): NOT, DEFINED
• Application Number:
  US06/629,653
• Time in Patent Office: 1,273 Days
• Field of Search: 435/30, 435/287, 435/292, 435/293, 435/294, 435/297, 435/298, 435/299, 435/300, 435/301, 435/805, 128/759, 604/358, 604/385 R, 604/385 A, 604/386, 428/316.6
• US Class Current: 435/309.4
• CPC Class Codes: C12M 23/04 Flat or tray type, drawers C12M23/10, C12M23/12, C12M23/16 take precedence
• CPC Class Codes: C12M 23/20 Material Coatings immunocoatings C12M25/00
• CPC Class Codes: C12M 33/14 with filters, sieves or membranes

Abstract

A device for replicating moist bacterial or other cell colonies including a rigid layer, a resilient backing layer secured to one side of the rigid layer and a cell colony transfer surface made from an open-cell, flexible foam which includes a hydrophilic material therein and which surface is absorbent, porous, compliant and exhibits less resilience than said backing layer.

Claims

1. A device for replicating bacterial or other cell colonies comprising a layered structure of

(i) a rigid layer means;

(ii) a backing layer made of flexible foam which is secured to one side of the rigid means;

(iii) a transfer layer which is attached to the side of the backing layer opposite from the rigid means and which is made from open-cell, porous, flexible foam; and

(iv) a hydrophilic material included within the transfer layer and at its exposed surface, the hydrophilic material-containing foam transfer layer being air-porous and compliant but less resilient than the foam backing layer.

2. The device of claim 1 wherein the rigid means is a flat stiff plate.

3. The device of claim 1 wherein the flexible backing layer is open-cell, porous foam.

4. The device of claim 3 wherein the open-cell, porous foam of the backing layer is polyurethane.

5. The device of claim 3 wherein the open-cell, porous foam is a foam having less than 100 pores per inch.

6. The device of claim 1 wherein the transfer layer is laminated to the backing layer.

7. The device of claim 1 wherein the open-cell, porous foam of the transfer layer is polyurethane.

8. The device of claim 1 wherein the foam transfer layer is a foam having about 100 pores per inch.

9. The device of claim 1 wherein the hydrophilic material includes an additional component as a binder, plasticizer, filler, or bulking agent.

10. The device of claim 1 wherein the hydrophilic material includes diatomite particles.

11. The device of claim 1 wherein the hydrophilic material is a hydrocolloid.

12. The device of claim 11 wherein the hydrocolloid is selected from the group consisting of locust bean gum, guar gum, calcium alginate, collagen, gelatin, carrageenan, agar, agarose and hydroxyethyl derivatives thereof, water-insoluble alkali salts of polyacrylate, and water-insoluble alkali salts of gelatinized starch-polyacrylonitrile graft copolymers.

13. The device of claim 12 wherein the hydrocolloid is a water-absorbent water-insoluble alkali salt of gelatinized-starch polyacrylonitrile graft copolymer wherein the molar ratio of gelatinized starch to saponified polyacrylonitrile is from about 1:

1.5 to 1;

9.

14. The device of claim 13 wherein the gelatinized starch polyacrylonitrile graft copolymer is dispersed in a water-based latex binder.