Element for electrophoresis

• US 4,718,998 A
• Filed: 05/19/1987
• Issued: 01/12/1988
• Est. Priority Date: 05/20/1986
• Status: Expired due to Term

• Current Assignee: Fuji Photo Film Co Limited (Fujifilm Holdings Corporation)
• Original Assignee: Fuji Photo Film Co Limited (Fujifilm Holdings Corporation)
• Inventors: Ogawa, Masashi, Sugimoto, Naohiko
• Primary Examiner(s): Niebling, John F.
• Assistant Examiner(s): STARSIAK, JOHN S
• Application Number:
  US07/052,579
• Time in Patent Office: 238 Days
• Field of Search: 204/299 R, 204/182.8, 428/516, 428/518, 428/520, 428/483
• US Class Current: 204/606
• CPC Class Codes: G01N 27/44747 Composition of gel or of carrier mixture
• CPC Class Codes: Y10T 428/31507 Of polycarbonate
• CPC Class Codes: Y10T 428/31797 Next to addition polymer from unsaturated monomers
• CPC Class Codes: Y10T 428/31913 Monoolefin polymer
• CPC Class Codes: Y10T 428/3192 Next to vinyl or vinylidene chloride polymer

Abstract

An element for electrophoresis suitably employable for analysis of biopolymers such as proteins, as well as for determination of base sequence of DNA, RNA, their fragments, and their derivatives, which comprises a plastic film, a layer for electrophoresis comprising an aqueous polyacrylamide gel, an adhesive layer containing gelatin and optionally a film-forming polymer and/or fine particles, and a plastic film.

Claims

1. An element for electrophoresis comprising:

(I) a plastic film;

(II) a layer for electrophoresis comprising an aqueous polyacrylamide gel formed by crosslinking polymerization of an acrylamide compound and a crosslinking agent in the presence of water;

(III) an adhesive layer containing gelatin; and

(IV) a plastic film, which are superposed in order.

2. The element for electrophoresis as claimed in claim 1, wherein said gelatin contained in the adhesive layer is a lime-treated gelatin.

3. The element for electrophoresis as claimed in claim 1, wherein the adhesive layer consisting essentially of gelatin.

4. The element for electrophoresis as claimed in claim 1, wherein said adhesive layer contains at least 5 wt. % of gelatin and further contains a film-forming material.

5. The element for electrophoresis as claimed in claim 1, wherein said adhesive layer contains at least 5 wt. % of gelatin and further contains a material selected from the group consisting of cellulose derivatives, dextran, agarose, polyacrylamide, polyvinyl alcohol esters, polyacrylate esters, pullulan, pullulan derivatives, polyvinylidene chloride, vinylidene chloride latex and water glass.

6. The element for electrophoresis as claimed in claim 1, wherein said adhesive layer contains at least 5 wt. % of gelatin and further contains a filler of fine particles.

7. The element for electrophoresis as claimed in claim 1, wherein said adhesive layer contains at least 5 wt. % of gelatin and further contains colloidal silica or methanol silica.

8. The element for electrophoresis as claimed in claim 1, wherein said medium layer contains a compound having at least one carbamoyl group.

9. The element for electrophoresis as claimed in claim 1, wherein said medium layer contains urea or formamide.

10. The element for electrophoresis as claimed in claim 1, wherein said medium layer contains a water-soluble polymer and agarose.

11. The element for electrophoresis as claimed in claim 1, wherein said medium layer contains an anionic surfactant.

12. The element for electrophoresis as claimed in claim 1, wherein said medium layer contains an alkylsulfate.

13. The element for electrophoresis as claimed in claim 1, wherein the plastic film (I) is thicker than the plastic film (IV).