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Enzyme-free diagnostic binding reagents

  • US 4,719,176 A
  • Filed: 10/31/1983
  • Issued: 01/12/1988
  • Est. Priority Date: 10/31/1983
  • Status: Expired due to Fees
First Claim
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1. An enzyme-free diagnostic reagent for determining the presence of an entity to be assayed in a homogeneous assay comprising an enzyme-free hydrolytic catalyst coupled by a linking group to a first binding agent, said catalyst having a molecular weight together with the linking group of about 75 to about 2000 daltons, wherein said catalyst catalyzes a hydrolytic chemical reaction with successive preselected hydrolyzable co-reactant molecules when said co-reactant molecules and said catalyst are admixed in homogenous solution and said catalyst is coupled to said first binding agent as said diagnostic reagent;

  • said diagnostic reagent specifically binding in aqueous medium to a second binding moiety to form a binding complex, said second binding moiety being (a) the entity to be assayed in a direct assay, or (b) an entity needed for the assay in an indirect assay, said diagnostic reagent indicating the amount of said second binding moiety present in said binding complex by means of the catalytic reactivity of said catalyst with said co-reactant molecules, said catalytic reactivity being changed when said binding complex is formed.

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