Cyclophilin

• US 4,722,999 A
• Filed: 05/03/1985
• Issued: 02/02/1988
• Est. Priority Date: 05/03/1985
• Status: Expired due to Fees

• Current Assignee: Yale University
• Original Assignee: Yale University
• Inventors: Harding, Matthew W., Speicher, David W., Handschumacher, Robert E.
• Primary Examiner(s): Kight, John
• Assistant Examiner(s): Draper, Garnette D.
• Application Number:
  US06/730,776
• Time in Patent Office: 1,005 Days
• Field of Search: 260/112 R, 260/112.5 R, 530/350, 530/324, 530/412, 530/413, 530/417, 530/415
• US Class Current: 530/412
• CPC Class Codes: A61K 35/26 Lymph; Lymph nodes; Thymus; Spleen; Splenocytes; Thymocytes
• CPC Class Codes: A61K 35/28 Bone marrow; Haematopoietic stem cells; Mesenchymal stem cells of any origin, e.g. adipose-derived stem cells
• CPC Class Codes: C07K 14/47 from mammals
• CPC Class Codes: C07K 14/705 Receptors; Cell surface antigens; Cell surface determinants tumour specific antigens C07K14/4748

Abstract

Homogeneous cyclophilin, a soluble binding protein, having a specific binding activity of above 50 ug cyclosporin A per mg protein and a molecular weight of about 17,600 daltons, reversibly binds immunosuppressants or antibodies thereto such as cyclosporin or anti-cyclophilin. It is isolated from thecytosol of several different mammalian tissues and can be used in various diagnostic and purifications procedures.

Claims

1. A method of preparing cyclophilin, a homogeneous cystolic binding protein having a specific binding activity above 50 ug cyclosporin A per mg of protein bound and a molecular weight of about 17,600 daltons by means of molecular weight exclusion columns isoelectric focusing which includes the step of contacting cyclophilin containing material with a chromatographic matrix of Cibacron Blue dye bound through a 12 carbon atom spacer arm to agarose, phenylsepharose chromatography and cationic chromatography.