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Cyclophilin

  • US 4,722,999 A
  • Filed: 05/03/1985
  • Issued: 02/02/1988
  • Est. Priority Date: 05/03/1985
  • Status: Expired due to Fees
First Claim
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1. A method of preparing cyclophilin, a homogeneous cystolic binding protein having a specific binding activity above 50 ug cyclosporin A per mg of protein bound and a molecular weight of about 17,600 daltons by means of molecular weight exclusion columns isoelectric focusing which includes the step of contacting cyclophilin containing material with a chromatographic matrix of Cibacron Blue dye bound through a 12 carbon atom spacer arm to agarose, phenylsepharose chromatography and cationic chromatography.

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