Method of assaying the presence of cancer cells
First Claim
1. A method of assaying the presence of cancer cells in a patient by measuring the level of assay-marker in a sample from the patient, comprising:
- (a) providing an antibody to the human analog of a protein secreted by the cell line ts B77 Rat 1 BIH, which cell line is deposited with the American Type Culture Collection, Rockville, Md. as deposit number CRL 8912, and wherein the assay-marker has the following characteristics;
(1) molecular weight 70,000-74,000;
(2) reacts with an antibody raised in rabbits directed against the rat analog-marker having a molecular weight of 62,000;
(3) cleaved by thrombin to fragments having an approximate molecular weight of 30,000;
(4) cleaved in the course of whole blood coagulation to fragments having a molecular weight of 30,000;
(5) binds to barium citrate; and
(6) binds to an anion cellulose column completely in 0.01M Na2 HPO4 at pH 6.8 and is eluted with 0.4M NaCl;
(b) reacting the antibody of step (a) with the sample to be tested; and
(c) measuring the level of reacted antibody to detect and/or determine the presence and/or quantity of cancer cells in the patient.
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Abstract
Method to detect and/or determine the presence of cancer cells in mammals, including humans, and to monitor the progress of treatment for cancer. A human protein marker, the assay-marker, has been ascertained of the molecular weight 70,000-74,000, which is secreted by cancer cells at levels 10-fold or greater than that observed with normal cells. An assay is described where the assay-marker or an antigenically analogous protein, the analog-marker, is used to prepare antibodies to the assay-marker. The antibodies are then reacted with blood or serum samples to determine the level of the assay-marker in the sample. Thus, the assay essentially comprises (1) providing an antibody to the protein described above (2) reacting the antibody of step 1 with the sample to be tested and (3) measuring the level of the reacted antibody to detect and/or determine the presence and/or quantity of cancer cells.
15 Citations
3 Claims
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1. A method of assaying the presence of cancer cells in a patient by measuring the level of assay-marker in a sample from the patient, comprising:
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(a) providing an antibody to the human analog of a protein secreted by the cell line ts B77 Rat 1 BIH, which cell line is deposited with the American Type Culture Collection, Rockville, Md. as deposit number CRL 8912, and wherein the assay-marker has the following characteristics; (1) molecular weight 70,000-74,000; (2) reacts with an antibody raised in rabbits directed against the rat analog-marker having a molecular weight of 62,000; (3) cleaved by thrombin to fragments having an approximate molecular weight of 30,000; (4) cleaved in the course of whole blood coagulation to fragments having a molecular weight of 30,000; (5) binds to barium citrate; and (6) binds to an anion cellulose column completely in 0.01M Na2 HPO4 at pH 6.8 and is eluted with 0.4M NaCl; (b) reacting the antibody of step (a) with the sample to be tested; and (c) measuring the level of reacted antibody to detect and/or determine the presence and/or quantity of cancer cells in the patient. - View Dependent Claims (2, 3)
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Specification