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Method of assaying the presence of cancer cells

  • US 4,725,538 A
  • Filed: 10/25/1985
  • Issued: 02/16/1988
  • Est. Priority Date: 10/25/1985
  • Status: Expired due to Fees
First Claim
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1. A method of assaying the presence of cancer cells in a patient by measuring the level of assay-marker in a sample from the patient, comprising:

  • (a) providing an antibody to the human analog of a protein secreted by the cell line ts B77 Rat 1 BIH, which cell line is deposited with the American Type Culture Collection, Rockville, Md. as deposit number CRL 8912, and wherein the assay-marker has the following characteristics;

    (1) molecular weight 70,000-74,000;

    (2) reacts with an antibody raised in rabbits directed against the rat analog-marker having a molecular weight of 62,000;

    (3) cleaved by thrombin to fragments having an approximate molecular weight of 30,000;

    (4) cleaved in the course of whole blood coagulation to fragments having a molecular weight of 30,000;

    (5) binds to barium citrate; and

    (6) binds to an anion cellulose column completely in 0.01M Na2 HPO4 at pH 6.8 and is eluted with 0.4M NaCl;

    (b) reacting the antibody of step (a) with the sample to be tested; and

    (c) measuring the level of reacted antibody to detect and/or determine the presence and/or quantity of cancer cells in the patient.

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