Method for direct saccharification of raw starch using enzyme produced by a basidiomycete belonging to the genus Corticium

• US 4,727,026 A
• Filed: 05/23/1986
• Issued: 02/23/1988
• Est. Priority Date: 11/26/1985
• Status: Expired due to Fees

• Current Assignee: Godo Shusei Co., Ltd. (Oenon Holdings Incorporated)
• Original Assignee: Godo Shusei Co., Ltd. (Oenon Holdings Incorporated)
• Inventors: Sasaki, Hiroshi, Kurosawa, Kazuhiko, Sawada, Masahiko, Takao, Shoichi
• Primary Examiner(s): Shapiro, Lionel M.
• Application Number:
  US06/866,296
• Time in Patent Office: 641 Days
• Field of Search: 435/96, 435/99, 435/203, 435/205
• US Class Current: 435/96
• CPC Class Codes: C12N 9/2428 Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
• CPC Class Codes: C12P 19/20 produced by the action of an exo-1,4 alpha-glucosidase, e.g. dextrose
• CPC Class Codes: Y10S 435/911 using fungi

Abstract

A method for the cooking-free saccharification of starch using an amylase produced by Corticium rolfsii AHU 9627 or its variants. According to the method, even a high viscous suspension of 10% (w/v) or more raw-corn starch is almost completely hydrolyzed within 8 hours. The saccharification is proceeded at a higher temperature and a lower pH compared with those in known methods utilizing other amylases which are able to hydrolyze uncooked starch, so that the propagation of the infectious bacteria which would affect the saccharifying efficiency can be avoided.

Claims

1. A method for the cooking-free saccharification of starch which comprises using an amylase produced by Corticium rolfsii AHU 9627 (International Deposit No. FERM BP-1033) or its variants.

2. A method according to claim 1, wherein a culture filtrate of the Corticium rolfsii AHU 9627 (International Deposit No. FERM BP-1033) or its variants is provided as the amylase.