Human immune interferon

• US 4,727,138 A
• Filed: 09/11/1985
• Issued: 02/23/1988
• Est. Priority Date: 10/19/1981
• Status: Expired due to Term

• Current Assignee: Genentech, Inc. (Roche Holding AG)
• Original Assignee: Genentech, Inc. (Roche Holding AG)
• Inventors: Gray, Patrick, Goeddel, David V.
• Primary Examiner(s): Martinell, James
• Application Number:
  US06/774,838
• Time in Patent Office: 895 Days
• Field of Search: 435/68, 435/70, 435/172.3, 435/91, 435/317, 435/253, 435/255, 435/256, 435/243, 435/240, 435/235, 435/241, 435/811, 435/172.1, 435/317.1, 435/240.2, 935/11, 935/27, 935/28, 935/29, 935/68-75, 935/60, 536/27, 530/320, 530/351
• US Class Current: 536/23.52
• CPC Class Codes: A61K 38/00 Medicinal preparations containing peptides peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00
• CPC Class Codes: A61P 31/12 Antivirals
• CPC Class Codes: A61P 35/00 Antineoplastic agents
• CPC Class Codes: A61P 43/00 Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
• CPC Class Codes: C07K 14/57 IFN-gamma
• CPC Class Codes: C12N 15/71 Expression systems using regulatory sequences derived from the trp-operon
• CPC Class Codes: C12N 15/81 for yeasts
• CPC Class Codes: C12N 15/85 for animal cells
• CPC Class Codes: C12P 21/02 having a known sequence of two or more amino acids, e.g. glutathione
• CPC Class Codes: Y10S 435/811 Interferon
• CPC Class Codes: Y10S 930/142 Interferon
• CPC Class Codes: Y10S 930/30 Signal or leader sequence

Abstract

Disclosed is a complete description of the preparation of novel, substantially pure polypeptide via recombinant DNA techniques utilizing any of an assortment of expression vectors and host cultures. The polypeptide, human immune (gamma) interferon (IFN-γ), is isolated and characterized in terms of DNA and amino acid sequences, physical attributes and biological activity.

Claims

1. A DNA molecule comprising a recombinant DNA molecule or a cDNA molecule encoding a polypeptide comprising the amino acid sequence:

##STR3##

3. A DNA molecule according to claim 1 or claim 2 operably linked with a DNA sequence capable of effecting expression of the DNA encoding said polypeptide.

2. A DNA molecule comprising a recombinant DNA molecule or a cDNA molecule encoding a polypeptide comprising the amino acid sequence:

##STR4##

4. A molecule consisting essentially of a DNA molecule encoding the amino acid sequence of recombinant human immune interferon.

5. A molecule consisting essentially of a DNA molecule encoding the amino acid sequence of des-CYS-TYR-CYS recombinant human immune interferon.