Implantable chemoattractant system

• US 4,732,155 A
• Filed: 08/27/1985
• Issued: 03/22/1988
• Est. Priority Date: 08/27/1985
• Status: Expired due to Fees

• Current Assignee: Children's Medical Center Corporation
• Original Assignee: Children's Medical Center Corporation
• Inventors: Zetter, Bruce R., Langer, Robert S.
• Primary Examiner(s): Rosen, Sam
• Application Number:
  US06/769,946
• Time in Patent Office: 938 Days
• Field of Search: 435/284, 128/632, 128/637, 128/630
• US Class Current: 600/300
• CPC Class Codes: A61K 9/0024 Solid, semi-solid or solidifying implants, which are implanted or injected in body tissue compositions for intravenous administration, normal injectable solutions or dispersions for, e.g. subcutaneous administration A61K9/0019; brain implants A61K9/0085; (coated) prostheses, catheters or stents A61L

Abstract

A system for providing sustained prolonged release of a chemoattractant to a surrounding biological medium which contains cells responsive to the chemoattractant. The system consists of a biocompatible, non-inflammatory body, which sequesters the chemoattractant and allows sustained prolonged release of the chemoattractant from at least one part of the body. A cell trap is positioned adjacent to the releasing portion of the body, so that the released chemoattractant is transmitted through the trap and attracted cells are trapped in it.

Claims

1. A device for providing sustained, prolonged release of a chemoattractant to cells in a surrounding biological medium and for trapping cells attracted by said chemoattractant, said device comprising:

(a) a biocompatible non-inflammatory body comprising said chemoattractant sequestered therein, said body providing sustained prolonged release of said chemoattractant from at least a portion thereof, and

(b) a cell trap comprising a non-inflammatory wettable material attached to said body, chemoattractant released from said body being transmitted through said trap, and cells attracted by said chemoattractant released from said body being trapped by and retained within said trap.

2. The device of claim 1 wherein said body comprises a polymer matrix impermeable to said cells and to said chemoattractant.

3. The device of claim 2 wherein said polymer is an ethylene vinyl acetate copolymer.

4. The device of claim 1 wherein said cell trap comprises a web which allows penetration by cells attracted to said chemical, said web being capable of being fixed and sectioned to examine said cells.

5. The device of claim 1 wherein said cell trap comprises a polymer having openings larger than said cells, said polymer exhibiting a surface attraction for said cells whereby cells entering said openings are retained in said trap.

6. The device of claim 5 wherein said surface attraction is electrostatic.

7. The device of claim 4 or 5 wherein said trap comprises a synthetic polyester fiber.

8. The device of claim 1 wherein said chemoattractant is chosen from:

(a) histamine

(b) tuftsin

(c) Glycyl-Histidyl-Lysine.

(d) Alanyl-Glycyl-Seryl-Glutamine

(e) f-Methionyl-Leucyl-Phenylalanine

(f) Glycyl-Histidyl-Glycine

(g) Valinyl-Glycyl-Seryl-Glutamine

(h) collagen Type I

(i) epidermal growth factor

(j) endotoxin standard

(k) prostaglandin E and

(1) heparin.

9. The device of claim 1 wherein said body is configured to provide directional release.

10. The device of claim 1 wherein only a portion of the surface of said body releases said chemoattractant and said portion comprises less than 1/4 of the surface area of said body to provide directional release of said chemoattractant.

11. The device of claim 1 wherein said cells comprise a heterogenous group of cells, and said trap is adapted to be sectioned to determine the cell species in said cell group that are trapped therein.

12. The device of claim 11 wherein said chemoattractant is a substance to be evaluated for allergenic activity.

13. The device of claim 11 wherein said cell group comprises neutrophiles, monocytes, and macrophages.

14. The device of claim 1 wherein said cell trap comprises polyester.

15. A method of measuring the ability of a chemoattractant to attract cells, send method comprising

providing the device of claim 1 comprising said chemoattractant sequestered in said body;

contacting said device with a medium comprising said cells; and

then

determining the presence of cells trapped in said trap.

16. The method of claim 15 wherein said contacting step comprises implanting said device within a living animal.