Analytical element for the detection hydrogen peroxide

• US 4,732,736 A
• Filed: 11/15/1985
• Issued: 03/22/1988
• Est. Priority Date: 11/20/1984
• Status: Expired due to Term

• Current Assignee: Konica Corporation (Konica Minolta Inc.)
• Original Assignee: Konishiroku Photo Industry Co., Ltd. (Konica Minolta Inc.), Chugai Seiyaku Kabushiki Kaisha (Roche Holding AG)
• Inventors: Okaniwa, Kenichiro, Zanma, Soichi, Koyama, Mikio, Kobayashi, Morio, Furukawa, Kunihiro
• Primary Examiner(s): Richman, Barry S.
• Assistant Examiner(s): Johnston, J.
• Application Number:
  US06/798,562
• Time in Patent Office: 858 Days
• Field of Search: 436/135, 436/170, 436/904, 422/56, 422/57, 422/58, 435/28, 435/805
• US Class Current: 422/428
• CPC Class Codes: C12Q 1/28 involving peroxidase
• CPC Class Codes: G01N 33/526 the element being adapted for a specific analyte
• CPC Class Codes: Y10S 435/805 Test papers
• CPC Class Codes: Y10S 436/904 Oxidation - reduction indicators
• CPC Class Codes: Y10T 436/206664 Ozone or peroxide

Abstract

An analytical element for the detection of hydrogen peroxide comprising a peroxidase-functional material, a hydrogen donor and a coupler, wherein the peroxidase-functional material and hydrogen donor are arranged so as to be separate from each other, for example in separate layers, but are made interactive by the application of a fluid sample.

Claims

1. In a multilayered analytical element for detecting hydrogen peroxide which comprises peroxidase-functional material, a hydrogen donor and a coupler, the improvement comprising arranging said peroxidase-functional material and said hydrogen donor in different layers in a manner so that they are separated from each other but are capable of interacting when a liquid sample is applied to the analytical element.

2. The analytical element of claim 1, wherein said hydrogen donor is selected from the group consisting of 4-substituted antipyrine compounds, 2-hydrazonobenzothiazoline compounds, p-halogenophenol compounds, and o- and p-phenylene diamine compounds.

3. The analytical element of claim 1, wherein said coupler is selected from the group consisting of acylacetoamide compounds, pyrazolone compounds, phenol compounds, naphthol compounds and N,N-di-substituted aniline compounds.

4. The analytical element of claim 1, wherein said analytical element comprises a support, and wherein one of said layers is a spreading layer.

5. The analytical element of claim 4, wherein one of said peroxidase-functional material and said hydrogen donor is contained in said spreading layer.

6. The analytical element of claim 1, wherein the content of said peroxidase-functional material is in the range of 100 U/m² to 1,000,000 U/m².

7. The analytical element of claim 6, wherein the content of said peroxidase-functional material is in the range of 1,000 U/m² to 100,000 U/m².

8. The analytical element of claim 1, wherein the content of said hydrogen donor is in the range of 0.01 m mol/m² to 100 m mol/m².

9. The analytical element of claim 8, wherein the content of said hydrogen donor is in the range of 0.05 m mol/m² to 50 m mol/m².

10. The analytical element of claim 1, wherein the content of said coupler is in the range of 0.1 m mol/m² to 100 m mol/m².

11. The analytical element of claim 10, wherein the content of said coupler is in the range of 0.5 m mol/m² to 50 m mol/m².