Flow chamber device for flow cytometers
First Claim
1. A flow chamber device to be employed in a flow cytometer facilitating that biological cells or particles, carried by a microscopical laminar fluid flow, are brought one by one across the open surface of a plane glass with a velocity which may be varied from above 30 m/sec. to below 0.01 m/sec. (1 cm/sec.), so that the fluorescence and light scattering of the cells/particles may be measured through optics situated on each side of the plane glass, characterized by the fact that the flow chamber comprises a nozzle (1) situated in contact with the open surface of a plane glass (2) having its axis at an oblique angle to the said glass (2) so that the orifice of the nozzle (4) is situated immediately adjacent to the glass surface and so that the flow from the orifice of the nozzle (4) connects this orifice with the glass surface through a meniscus of fluid maintained independently of the flow velocity.
0 Assignments
0 Petitions
Accused Products
Abstract
The present invention relates to a device which facilitates that biological cells and other microscopical particles, carried by a microscopial laminar fluid flow, are brought one by one across the open surface of a plane glass with a velocity which may be varied from above 30 m/sec. to below 1 cm/sec., so that the fluorescence and light scattering of the cells/particles may be measured through optics situated on each side of the plane glass. The device comprises a nozzle (1) with hydrodynamic focusing having its axis at an oblique angle to said glass surface. Furthermore, at the same time the electrical volume of the cells/particles may be determined by means of an electrode, made of inert metal, situated in the contact plane between the nozzle (1) and said glass (2) and an electrode situated in the fluid supply (8) of the nozzle (1). By retaining a constant electrolytical current between the electrode (6) and the electrode (7) the electrical volume of the cells/particles may be measured by the voltage pulses produced between the said electrodes when the cells/particles pass through the orifice.
56 Citations
8 Claims
- 1. A flow chamber device to be employed in a flow cytometer facilitating that biological cells or particles, carried by a microscopical laminar fluid flow, are brought one by one across the open surface of a plane glass with a velocity which may be varied from above 30 m/sec. to below 0.01 m/sec. (1 cm/sec.), so that the fluorescence and light scattering of the cells/particles may be measured through optics situated on each side of the plane glass, characterized by the fact that the flow chamber comprises a nozzle (1) situated in contact with the open surface of a plane glass (2) having its axis at an oblique angle to the said glass (2) so that the orifice of the nozzle (4) is situated immediately adjacent to the glass surface and so that the flow from the orifice of the nozzle (4) connects this orifice with the glass surface through a meniscus of fluid maintained independently of the flow velocity.
-
8. A flow chamber device to be employed in a flow cytometer facilitating that biological cells or particles, carried by a microscopical laminar fluid flow are brought one by one across the open surface of a plane glass with a velocity which may be varied from above 30 m/sec. to below 0.01 m/sec. (1 cm/sec.), so that the fluorescence and light scattering of the cells/particles may be measured through optics situated on each side of the plane glass while at the same time the electrical volume of the cells/particles can be determined, characterized by the fact that the flow chamber comprises a nozzle (1) situated in contact with the open surface of a plane glass (2) having its axis at an oblique angle to the said glass (2) so that the orifice of the nozzle (4) is situated immediately adjacent to the glass surface and so that the fluid flow from the nozzle orifice (4) connects the orifice with the glass surface through a meniscus of fluid which is maintained independently of the flow velocity, and that the electrical volume of the cells/particles is determined by means of an electrode (6) of inert metal situated in the plane of contact between said glass (2) and said nozzle (1), while an electrode (7) is situated in the fluid supply (8) of the nozzle (1) so that a constant electrolytical current between said electrodes (6) and (7) is maintained through the nozzle orifice (4) in order to facilitate measurement of the electrical volume of the particles passing through the orifice (4) of said nozzle (1).
Specification