Element for electrophoresis

• US 4,737,259 A
• Filed: 12/29/1986
• Issued: 04/12/1988
• Est. Priority Date: 03/15/1984
• Status: Expired due to Fees

• Current Assignee: Fuji Photo Film Co Limited (Fujifilm Holdings Corporation)
• Original Assignee: Fuji Photo Film Co Limited (Fujifilm Holdings Corporation)
• Inventors: Mizobuchi, Yuzo, Ogawa, Masashi, Hashiue, Masakazu
• Primary Examiner(s): NOT, DEFINED
• Application Number:
  US06/948,401
• Time in Patent Office: 470 Days
• Field of Search: 204/299 R, 204/182.8, 428/477.7, 428/448
• US Class Current: 204/606
• CPC Class Codes: G01N 27/44747 Composition of gel or of carrier mixture
• CPC Class Codes: Y10T 428/31765 Inorganic-containing or next to inorganic-containing

Abstract

An element for electrophoresis suitably employable for electrophoresis of biopolymers such as proteins, as well as for the determination of the base sequence of DNA, RNA, their fragments, and their derivatives, which comprises a plastic support, a nonconductive metal oxide layer, and an electrophoresis medium layer comprising an aqueous polyacrylamide gel formed by crosslinking polymerization of an acrylamide compound and a crosslinking agent in the presence of water, which are superposed in this order. The electrophoresis medium layer may contain a water-soluble polymer and agarose. The medium layer may contain a modifier such as an anionic surfactant, formamide or urea.

Claims

1. An element for electrophoresis comprising in the following order:

(I) a plastic material support layer;

(II) a nonconductive oxide layer; and

(III) a gel membrane for electrophoresis comprising an aqueous polyacrylamide gel formed by crosslinking polymerization of an acrylamide compound and a crosslinking agent in the presence of water.

2. The element for electrophoresis as claimed in claim 1, in which said nonconductive oxide layer is selected from the group consisting of SiO₂, TiO₂, MgO, Al₂ O₃, BeO, ZrO₂, WO₂, and Ta₂ O₃.

3. The element for electrophoresis as claimed in claim 2, in which said nonconductive oxide is selected from the group consisting of SiO₂ and TiO₂.

4. The element for electrophoresis as claimed in claim 1, in which said nonconductive oxide layer is treated with a vinyl silane on its surface.

5. The element for electrophoresis as claimed in any one of claims 1 to 4, in which the plastic material support layer is a polyethylene terephthalate sheet.

6. The element for electrophoresis as claimed in any one of claims 1 to 4, in which said gel membrane contains a water-soluble polymer and agarose.

7. The element for electrophoresis as claimed in any one of claims 1 to 4, in which said gel membrane contains an anionic surfactant.

8. The element for electrophoresis as claimed in claim 7, in which said anionic surfactant is an alkylsulfate.

9. The element for electrophoresis as claimed in claim 8, in which said alkylsulfate is sodium dodecylsulfate.

10. The element for electrophoresis as claimed in any one of claims 1 to 4, in which said gel membrane contains a compound having at least one carbamoyl group.

11. The element for electrophoresis as claimed in claim 10, in which said compound having at least one carbamoyl group is urea or formamide.