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Reducing interference in ligand-receptor binding assays

  • US 4,737,456 A
  • Filed: 05/09/1985
  • Issued: 04/12/1988
  • Est. Priority Date: 05/09/1985
  • Status: Expired due to Term
First Claim
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1. A method for assaying for a ligand which is a member of a specific binding pair ("sbp member ") consisting of said ligand and its complementary receptor wherein said ligand has a binding site in common with an interfering substance present in a sample suspected of containing said ligand, said interfering substance having at least two binding sites, which method comprisescombining without intervening separation (1) said sample with (2) a blocking receptor, which does not bind to said ligand and does bind to said interfering substance and thereby blocks binding of a common receptor to said interfering substance, and (3) a common receptor which binds to said common binding site, to provide an assay medium, anddetermining the extent of binding of said common receptor, the extent of binding being related to the amount of said analyte in said sample.

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