Cuvette handling

• US 4,738,825 A
• Filed: 02/27/1985
• Issued: 04/19/1988
• Est. Priority Date: 02/27/1985
• Status: Expired due to Fees

• Current Assignee: Il Holding SpA (Werfen SA)
• Original Assignee: Fisher Scientific Co. (Thermo Fisher Scientific Incorporated)
• Inventors: Nelson, Larry A., Kelln, Norman G., Tiffany, Thomas O.
• Primary Examiner(s): Marcus, Michael S.
• Application Number:
  US06/706,073
• Time in Patent Office: 1,147 Days
• Field of Search: 422/64-67, 422/72, 422/73, 422/102
• US Class Current: 422/72
• CPC Class Codes: G01N 2035/00306 Housings, cabinets, control panels (details)
• CPC Class Codes: G01N 2035/00316 Detecting door closure
• CPC Class Codes: G01N 2035/00495 Centrifuges
• CPC Class Codes: G01N 2035/00752 bar codes
• CPC Class Codes: G01N 2035/0448 composed of interchangeable ring elements
• CPC Class Codes: G01N 35/02 using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations G01N35/0098 and G01N35/0099 take precedence

Abstract

An analysis system of the concurrent type has a multicuvette unit supply station, a loading station, an analysis station, and transport mechanism for transporting cuvette units in correlated orientation between the several stations. Each cuvette unit includes mechanical interlock structure that cooperates with orientation structure at each of the several stations and with orientation structure carried by the transport mechanism so that continuity of cuvette orientation information is maintained throughout loading and concurrent analysis of the several samples in the cuvette unit.

Claims

1. A chemical analysis system comprising a supply station for furnishing a series of multicuvette centrifugal analyzer rotor units, each rotor unit including interlock structure,

first rotor orienting means at said supply station for engaging interlock structure of a rotor unit for maintaining a rotor unit in predetermined orientation,

a rotor unit loading station,

second rotor orienting means at said loading station for engaging interlock structure of each rotor unit for positioning a rotor unit in predetermined orientation relative to loading apparatus as said loading station;

means at said loading station for loading a rotor unit with sample and reagent materials,

an analysis station for performing analyses on the contents of a plurality of cuvettes of a rotor unit concurrently,

third rotor orienting means at said analysis station for engaging interlock structure of a rotor unit for positioning a rotor unit in predetermined orientation relative to said analysis station,

means at said analysis station for concurrently analyzing the contents of a rotor unit at said station,

and transport mechanism movable along a closed loop path between said supply, loading and analysis stations for transporting rotor units in correlated orientation between said stations, said transport mechanism including rotor unit carrier means for picking up a rotor unit at any of said stations and transporting the picked up rotor unit to another station for deposit in engagement with rotor unit orienting means at that station, said carrier means having fourth rotor orienting means for engaging interlock structure of a rotor unit for providing rotor unit orientation correlated to rotor unit orientations at said supply, loading and analysis stations.

2. The analyser system of claim 1 and further including at least one centrifugal analyzer rotor unit, each said rotor unit having centrally located interlock structure and peripherally located interlock structure, said fourth rotor orienting means being adapted to engage said peripherally located interlock structure to maintain orientation of the rotor unit between stations.

3. The system of claim 2 wherein each said rotor defines a circumferential array of elongated radially extending cuvettes, each said elongated cuvette including structure defining a first chamber for receiving a first constituent and a loading port through which said first constituent is introduced into said first chamber, structure defining a second chamber region for receiving a second constituent, a second loading port through which said second constituent is introduced into said second chamber region, divider structure between said first and said chamber regions that defines a transfer passage between said first and second chamber regions through which said first constituent may be flowed into said second chamber region for forming a reaction product with said second constituent, and structure defining an analysis region adjacent the radially outer wall of said cuvette where said reaction product is subjected to analysis.

4. The system of claim 3 further including drive means at said analysis station for spinning a rotor at at least one thousand rpm for mixing sample and reagent materials in separate compartments of the several cuvettes.

5. The system of claim 4 further including rotor support means with centrifugally actuated latch means at said analysis station for securing a rotor on said support means while it is being spun by said drive means.

6. The system of claim 3 wherein said analysis station includes a table for receiving a cuvette rotor, said third rotor orienting means being adapted to engage said interlock structure to maintain orientation of the rotor at said analysis station, and sensor means for providing output signals indicative of the angular position of said rotor at said analysis station.

7. The system of claim 1 further including feeder means for supporting rotor units in stacked relation at said supply station, and release means responsive to said transport mechanism for releasing said rotor units sequentially from feeder means.

8. The system of claim 7 wherein said first rotor orienting means includes rail structure that extends along the length of said feeder means for engagement with interlock structure of rotor units to maintain the rotor units in the stack at said supply station in oriented relation one to another.

9. The system of claim 1 and further including a discard station that includes means for storing used rotor units in stacked relation and said transport mechanism is movable between said loading and analysis stations and said discard station for disposing of rotor units after analysis of their contents.

10. The system of claim 9 wherein said used rotor unit receiving means includes an elongated shaft portion having orientation structure corresponding to the interlock structure of a rotor unit so that a plurality of rotor units may be stored on said shaft in angularly aligned relation, latch structure at one end of said shaft, and latch release structure for releasing the stack of rotor units on said shaft.

11. A chemical analysis system comprising

a supply station for furnishing a series of multicuvette centrifugal analyzer rotor units, each rotor unit including centrally located interlock structure and peripherally located interlock structure,

feeder means for supporting rotor units in stacked relation at said supply station,

first rotor orienting means at said supply station for maintaining rotor units in predetermined orientation, said first rotor orienting means including rail structure that extends along a length of said feeder means, said rail structure being adapted to engage a peripherally located interlock structure of rotor units to maintain rotor units in the stack at said supply station in oriented relation one to another,

a rotor unit loading station,

second rotor orienting means at said loading station for engaging centrally located interlock structure of a rotor unit for positioning a rotor unit in predetermined orientation relative to loading apparatus at said loading station;

means at said loading station for loading cuvettes of a rotor unit with sample and reagent materials,

an analysis station for performing analyses on the contents of a plurality of cuvettes of a rotor unit concurrently,

third rotor orienting means at said analysis station for engaging centrally located interlock structure of a rotor unit for positioning a rotor unit in predetermined orientation relative to said analysis station,

means at said analysis station for concurrently analyzing the contents of a rotor unit at said analysis station,

and transport mechanism movable along a closed loop path between said supply, loading and analysis stations for transporting rotor units in correlated orientation between said stations, said transport mechanism including rotor unit carrier means for picking up a rotor unit at any of said stations and transporting the picked up rotor unit to another station for deposit in engagement with the rotor orienting means at that station, said carrier means having fourth rotor orienting means for engaging peripherally located interlock structure of a rotor unit for providing rotor unit orientation correlated to the rotor unit orientations at said supply, loading and analysis stations, and release means responsive to said transport mechanism for and releasing rotor units sequentially from said feeder means.

12. The system of claim 11 further including drive means at said loading station for indexing cuvettes of a rotor unit past said loading means for sequential loading of sample and reagent materials into cuvettes of said a rotor unit.

13. The system of claim 12 wherein said loading station includes a table for receiving a rotor unit, and sensor means for providing output signals indicative of the angular position of a rotor unit at said loading station.

14. A chemical analysis system comprising a plurality of multicuvette centrifugal analyzer rotor units,

a supply station for furnishing said multicuvette centrifugal analyzer rotor units in series, each said rotor unit having a body portion with at least one orientation element at a periphery of said body portion and interlock structure,

first rotor orienting means at said supply station for engaging interlock structure of said rotor units for maintaining said rotor units in predetermined orientation,

a rotor unit loading station,

second rotor orienting means at said loading station for engaging interlock structure of said rotor units for positioning a rotor unit in predetermined orientation relative to loading apparatus at said loading station;

means at said loading station for loading a rotor unit with sample and reagent materials,

an analysis station for performing analyses on the contents of a plurality of cuvettes of a rotor unit concurrently,

third rotor orienting means at said analysis station for engaging interlock structure of said rotor unit for positioning a rotor unit is predetermined orientation relative to said analysis station,

means at said analysis station for concurrently analyzing the contents of the rotor unit at said analysis station,

a discard station including means for storing used rotor units in stacked relation, said used rotor storing means including an elongated shaft portion having orientation structure corresponding to the interlock structure of said rotors so that a plurality of rotor units may be stored on said shaft portion in angularly aligned relation, latch structure at one end of said shaft, and latch release structure for releasing the stack of rotors on said shaft portion,

and transport mechanism movable along a closed loop path between said supply, loading analysis and discard stations for transporting rotor units in correlated orientation between said stations, said transport mechanism including rotor support means and fourth rotor orienting means including an orientation member carried by said support means for mechanically engaging said orientation element to maintan the rotor in desired orientation during transport of said rotor by said support means between said stations.

15. The system of claim 14 wherein said rotor support mechanism includes a pair of support arms, and said system includes acutator mechanism at each of a plurality of said station for operating said support arms to grasp and release rotors.

16. An analysis system comprising structure defining an analysis compartment, means for maintaining said analysis compartment at a controlled and stabilized temperature at which analyses are to be performed,

supply, loading, holding and analysis stations in said compartment,

means for storing a plurality of multicuvette centrifugal analyzer rotor units of long thermal time constant material in said compartment adjacent said supply station,

each said rotor unit having a body portion that defines a circumferential array of elongated cuvettes, and interlock structure,

each of said loading, holding and analysis stations including a rotor unit support table that has a radially extending support surface, and an orienting member on said support surface for mechanical engagement with a rotor unit interlock structure to establish orientation of a rotor unit on said table,

a transfer mechanism adjacent said loading station for loading predetermined quantities of sample and reagent into a plurality of cuvettes in a rotor unit on said support table of said loading station, and

a transport mechanism in said compartment adapted to transport loaded rotor units selectively between said loading station and said holding and analysis stations,

said transport mechanism including orientation means for mechanically engaging a rotor unit to maintain a rotor unit in desired orientation during transport.

17. The system of claim 16 further including drive means at said loading station for indexing the cuvettes of a rotor unit on said support table past said transfer mechanism for sequential loading of sample and reagent materials into cuvettes of said rotor unit.

18. The system of claim 16 wherein said storing means includes feeder means for supporting said rotor units in stacked spaced relation at said supply station, and release means responsive to said transport mechanism for releasing said rotor units sequentially from said feeder means.

19. The system of claim 18 and further including a discard station, said transport mechanism being movable between said loading and analysis stations and said discard station for disposing of rotor units after analysis of their contents.

20. An analysis system comprising structure defining an analysis compartment, means for maintaining said analysis compartment at a controlled and stabilized temperature at which analyses are to be performed,

supply, loading, holding, discard and analysis stations in said compartment,

means for storing a plurality of multicuvette centrifugal analyzer rotor units of long thermal time constant material in said compartment adjacent said supply station, said storing means includes feeder means for supporting rotor units in stacked spaced relation at said supply station,

each said rotor unit having a body portion that defines a circumferential array of elongated cuvettes,

a transfer mechanism adjacent said loading station for loading predetermined quantities of sample and reagent into a plurality of cuvettes in a rotor unit at said loading station, and

a transport mechanism in said compartment adapted to transport loaded rotor units selectively between said loading station and said holding and analysis stations,

said transport mechanism being movable between said analysis station and said discard station for disposing of rotor units after analysis of their contents,

a rotor unit manipulating tool that includes post structure for storing a stack of rotor units, and

support structure at said discard station for fixedly supporting said manipulating tool so that said transport mechanism may load used rotor units onto said post structure for disposal.

21. An analysis system comprising structure defining an analysis compartment, means for maintaining said analysis compartment at a controlled and stabilized temperature at which analyses are to be performed,

supply, loading, holding, discard and analysis stations disposed in a circle in said compartment,

means for storing a plurality of multicuvette centrifugal analyzer rotor units of long thermal time constant material in said compartment adjacent said supply station, said storing means includes feeder means for supporting rotor units in stacked spaced relation at said supply station,

each said rotor unit having a body portion that defines a circumferential array of elongated cuvettes,

a transfer mechanism adjacent said loading station for loading predetermined quantities of sample and reagent into a plurality of cuvettes in a rotor unit, and

a transport mechanism in said compartment, said transport mechanism being mounted for rotation about the center of said circle and adapted to transport loaded rotor units selectively between said loading station and said holding and analysis stations and to transport rotor units from said analysis station to said discard station for disposing of rotor units after analysis of their contents.

22. The system of claim 21 further including drive means at said analysis station for spinning a rotor unit at at least one thousand rpm for mixing sample and reagent materials in the several cuvettes of said rotor unit, and sensor means for providing output signals indicative of the angular position of said rotor unit at said analysis station.

23. The system of claim 22 further including rotor unit support means with means at said analysis station for securing a rotor unit on said support means while it is being spun by said drive means.

24. The system of claim 23 further including drive means at said loading station for indexing the cuvettes of a rotor unit on said support table past said transfer mechanism for sequential loading of sample and reagent materials into cuvettes of said rotor unit, and sensor means for providing output signals indicative of the angular position of said rotor unit at said loading station.

25. The system of claim 24 wherein said transport mechanism includes a pair of support arms, and said system includes actuator mechanism at each of a plurality of said stations for operating said support arms to grasp and release rotor units.

26. The system of claim 25 and wherein said discard station is in alignment with said holding station, said discard station includes means for storing used rotor units in stacked relation, and said transport mechanism is movable between said analysis station and said discard station for inserting rotor units into said used rotor storing means after analysis of their contents.

27. A centrifugal analyzer system comprising:

structure defining an analysis compartment, means for maintaining said analysis compartment at a controlled and stabilized temperature at which analyses are to be performed,

supply loading, holding and analysis stations disposed along a circular path in said compartment,

a plurality of centrifugal analyzer rotors of long thermal time constant material,

means for storing said plurality of rotors in said compartment adjacent said supply station, each said rotor having interlock structure and a body portion that defines a circumferential array of elongated cuvettes, each said cuvette having an analysis region adjacent the radial periphery of said rotor,

each of said loading, holding and analysis stations including a rotor support table that has a radially extending rotor support surface and an orienting member on said support surface for mechanical engagement with said rotor interlock structure to establish rotor orientation of said rotor on said support table;

means at said loading station for loading individual cuvettes of a centrifugal analyzer rotor with sample and reagent material, and drive means at said loading station for indexing the cuvettes of a rotor for sequential loading of sample and reagent materials into cuvettes of said rotor;

drive means at said analysis station for spinning the rotor support table at said analysis station at at least one thousand rpm for mixing sample and reagent materials in the cuvettes of the rotor on the rotor support table and disposing the resulting mixtures of sample and reagent material in the respective analysis regions of the cuvettes at the periphery of said rotor, and sensor means at said analysis station for performing analyses on the sample-reagent mixtures in the analysis regions of the rotor concurrently as said rotor is being spun by said drive means;

transport mechanism in said compartment including rotor carrier structure, means to move said rotor carrier structure, means to move said rotor carrier structure along said circular path for transporting centrifugal analyzer rotors sequentially from said supply station to said loading station and for transporting loaded centrifugal analyzer rotors selectively between said loading station and said holding and analysis stations.

28. The system of claim 27 wherein said transport mechanism includes means to move said rotor carrier structure along said circular path in a first plane, said supply station including rotor feeder mechanism located above said first plane for storing a stack of rotors, and the rotor support tables at said loading, holding, and analysis stations being located in a second plane below said first plane,

means carried by said transport mechanism for releasing a rotor from said feeder mechanism as said rotor carrier is raised up towards said stack at said supply station, and

actuator means operative when said rotor carrier structure is at said loading, holding, and analysis stations in a position below said first plane for operating said carrier mechanism to release a rotor and deposit on the support table with the interlock structure in engagement with said orienting member.

29. The system of claim 28 further including rotor support means with means at said analysis station for securing a rotor on said support means while it is being spun by said drive means.

30. The system of claim 29 further including drive means at said loading station for indexing the cuvettes of a rotor on said support table past said transfer mechanism for sequential loading of sample and reagent materials into cuvettes of said rotor, and sensor means for providing output signals indicative of the angular position of said rotor at said loading station.