Method and apparatus for automated processing and aliquoting of whole blood samples for analysis in a centrifugal fast analyzer

• US 4,740,472 A
• Filed: 08/05/1985
• Issued: 04/26/1988
• Est. Priority Date: 08/05/1985
• Status: Expired due to Fees

• Current Assignee: The United States of America As Represented By The Secretary of Agriculture
• Original Assignee: United States Department of Energy
• Inventors: Johnson, Wayne F., Burtis, Carl A., Walker, William A.
• Primary Examiner(s): Richman, Barry S.
• Assistant Examiner(s): Kummert, Lynn M.
• Application Number:
  US06/762,368
• Time in Patent Office: 995 Days
• Field of Search: 436/45, 436/63, 436/177, 422/72, 422/101, 210/512.1, 210/512.3, 210/787, 494/16, 494/17, 494/22
• US Class Current: 436/63
• CPC Class Codes: B04B 5/0414 comprising test tubes
• CPC Class Codes: G01N 21/07 Centrifugal type cuvettes G01N21/09 takes precedence
• CPC Class Codes: Y10T 436/111666 Utilizing a centrifuge or compartmented rotor
• CPC Class Codes: Y10T 436/25375 Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]

Abstract

A rotor and disc assembly for use in a centrifugal fast analyzer. The assembly is designed to process multiple samples of whole blood followed by aliquoting of the resultant serum into precisely measured samples for subsequent chemical analysis. The assembly requires minimal operator involvement with no mechanical pipetting. The system comprises (1) a whole blood sample disc, (2) a serum sample disc, (3) a sample preparation rotor, and (4) an analytical rotor. The blood sample disc and serum sample disc are designed with a plurality of precision bore capillary tubes arranged in a spoked array. Samples of blood are loaded into the blood sample disc in capillary tubes filled by capillary action and centrifugally discharged into cavities of the sample preparation rotor where separation of serum and solids is accomplished. The serum is loaded into the capillaries of the serum sample disc by capillary action and subsequently centrifugally expelled into cuvettes of the analytical rotor for analysis by conventional methods.

Claims

1. A system for preparing blood samples for analysis, comprising:

a whole blood sample disc having a plurality of first radial passageways arranged therein in spoked array, each said first passageway removably receiving a capillary tube for a whole blood sample;

a serum sample disc removably secured to said whole blood sample disc and having a plurality of second radial passageways arranged therein in spoked array, each said second passageway removably receiving a capillary tube;

a sample preparation rotor having a center cavity for receiving and removably holding said whole blood and said serum sample discs, where the serum sample and whole blood sample discs are rotatable with the rotor, said whole blood sample disc being positioned on said rotor below said serum sample disc, said rotor including a plurality of separate cavities at its outer periphery, said first and said second radial passageways of said whole blood and said serum sample discs being oriented such that a separate whole blood sample capillary tube is aligned with a separate serum sample capillary tube to define a pair, each said pair of capillary tubes being in fluid communication with one of said separate cavities; and

means for rotating said sample preparation rotor to separate a plurality of whole blood samples into serum and solids in said separate cavities;

wherein said cavities and said serum capillary tubes are of sufficient size such that said serum migrates by capillary action from said separate cavities into said serum sample capillaries after rotation of the apparatus is halted.

2. A system as claimed in claim 1, wherein each pair of capillary tubes communicates with one of said separate cavities by means of a radial slot in said rotor, each of said radial slots positioned radially inward from, and communicating with, a separate cavity.

3. A system as claimed in claim 1, wherein the serum sample disc is secured on top of the whole blood sample disc by means of a raised circular section in the whole blood sample disc which mates with an opening at the center of the serum sample disc.

4. A system as claimed in claim 1, wherein the sample preparation rotor includes a fixed top plate for enclosing the cavities and passageways.

5. A system as claimed in claim 1, said system further including:

an analytical rotor having a center cavity for removably receiving said serum sample disc and serum sample capillaries, and having a plurality of cuvettes and means for dispensing a measured amount of analytical reagent to each of said cuvettes, each of said serum sample capillaries being in fluid communication with a separate cuvette; and

means for rotating said analytical rotor to effect transfer of the serum from said serum sample capillaries to said separate cuvettes for mixing with said dispensed analytical reagent.

6. A system as claimed in claim 5, wherein the whole blood sample disc and the serum sample disc each include means for atmospherically venting the whole blood and serum sample capillaries.

7. A system as claimed in claim 1, wherein the whole blood and the serum capillary tubes are held in place in their respective discs by means of o-rings.

8. A system as claimed in claim 7, wherein each o-ring is located at the bottom of a circular groove in each disc, each circular groove passing transversely across the respective radial passageways.

9. A system as claimed in claim 5, wherein the means for dispensing analytical reagent comprises a plurality of reagent cavities, each said reagent cavity in fluid communication with one of said separate cuvettes, the reagent being transferred to each said cuvette during rotation of the analytical rotor.

10. A system as claimed in claim 9, wherein the analytical rotor includes a fixed top cover containing a plurality of radial slots for accommodating the serum capillary tubes.

11. A system as claimed in claim 10, wherein the fixed top cover of the analytical rotor further includes a plurality of openings passing through the cover, each opening communicating with one of said plurality of reagent cavities.

12. A method for preparing blood samples for analysis, comprising the steps of:

obtaining whole blood samples in a plurality of capillary tubes;

placing each capillary tube in a separate radial passageway of a whole blood sample disc;

placing the whole blood sample disc in a sample preparation rotor;

disposing a serum sample disc above the whole blood sample disc, the serum sample disc having a plurality of empty serum capillary tubes received within radial passageways;

aligning each empty serum capillary tube with a separate whole blood sample capillary tube to form a pair;

aligning each pair of capillary tubes with a separate cavity along the periphery of the sample preparation rotor;

rotating the sample preparation rotor to effect transfer of the whole blood samples from the capillaries to the cavities by means of the centrifugal force generated by rotation of the rotor;

centrifugally separating the whole blood samples in the cavities into serum and solids by continued rotation of the rotor;

stopping rotation and allowing the serum to migrate by capillary action into the serum capillary tubes;

removing the serum sample disc from the sample preparation rotor and placing it in an analytical rotor, said analytical rotor containing a plurality of cuvettes and a means for dispensing analytical reagent into said cuvettes, each serum capillary tube aligned with a separate cuvette and in fluid communication therewith;

rotating the analytical rotor to effect transfer of the serum samples from the capillaries to the cuvettes by means of the centrifugal force generated by rotation of the rotor;

transferring analytical reagent to each cuvette, the serum samples being mixed with the analytical reagent by continued rotation of the analytical rotor; and

analyzing the serum samples.