Solid phase assay
First Claim
Patent Images
1. A process for assaying for an analyte, comprising:
- contacting a binder supported on a test area of a solid support with analyte and a tracer, said binder being a specific binder for at least the analyte, said tracer being comprised of a ligand labeled with a liposome, said liposome including a detectable marker which is not visible, said ligand being bound to one of the binder on the support or the analyte bound to the binder on the support, said test area being formed of a material having a surface area for supporting the binder and said binder being supported in the test area in a concentration of at least 1 ug/cm and at which tracer may be determined without lysis of the liposome; and
determining the tracer bound in said test area as a measure of analyte in a sample.
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Accused Products
Abstract
Solid phase assay for an analyte wherein binder is supported on a solid support, such as nitrocellulose, and the tracer is comprised of ligand labeled with a particulate label, such as a liposome, including a detectable marker which is not visible.
151 Citations
34 Claims
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1. A process for assaying for an analyte, comprising:
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contacting a binder supported on a test area of a solid support with analyte and a tracer, said binder being a specific binder for at least the analyte, said tracer being comprised of a ligand labeled with a liposome, said liposome including a detectable marker which is not visible, said ligand being bound to one of the binder on the support or the analyte bound to the binder on the support, said test area being formed of a material having a surface area for supporting the binder and said binder being supported in the test area in a concentration of at least 1 ug/cm and at which tracer may be determined without lysis of the liposome; and determining the tracer bound in said test area as a measure of analyte in a sample. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14)
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15. A process for assaying for an analyte, comprising:
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contacting a binder supported on a test area of a solid support with analyte and a tracer, said binder being a binder specific for at least the analyte, said tracer being comprised of a ligand labled with a liposome including a detectable marker which is not visible, said ligand being bound to one of the binder on the support or the analyte bound to the binder on the support, said test area being formed of nitrocellulose, said binder being supported in the test area in a concentration of at least 1 ug/cm and at which tracer may be determined without lysis of the liposome; and determining the tracer bound in said test area as a measure of analyte in a sample. - View Dependent Claims (16, 17, 18, 19, 20, 21, 22, 23, 24)
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25. A reagent kit for determining an analyte comprising:
a solid support including a binder on a test area of a solid support; and
a tracer, said binder being a binder specific for at least the analyte, said tracer being comprised of a ligand labeled with a liposome containing a detectable marker which is not visible, said ligand being bound to one of the binder on the support or the analyte bound to the binder on the support during an assay, said test area being formed of a material having a surface area supporting the binder and the binder being supported in a concentration of at least 1 ug/cm and at which tracer may be determined without lysis of the liposome.- View Dependent Claims (26, 27, 28, 29, 30, 31)
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32. A process for assaying for an analyte, comprising:
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contacting a binder supported on a test area of a solid support with analyte and a tracer, said binder being a specific binder for at least the analyte, said tracer being comprised of a ligand labeled with a lipsome, said liposome including a detectable marker which is not visible, said test area being formed of a material having a surface area for supporting the binder and the binder being supported in the test area in a concentration of at least 1 ug/cm and at which tracer may be determined without lysis of the liposome; and determining the tracer bound in said test area as a measure of analyte in a sample. - View Dependent Claims (33, 34)
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Specification