Process for encapsulation and encapsulated active material system

• US 4,744,933 A
• Filed: 08/16/1985
• Issued: 05/17/1988
• Est. Priority Date: 02/15/1984
• Status: Expired due to Term

• Current Assignee: Massachusetts Institute of Technology
• Original Assignee: Massachusetts Institute of Technology
• Inventors: Rodriguez-Sanchez, Dolores, Rha, ChoKyun
• Primary Examiner(s): Lovering, Richard D.
• Application Number:
  US06/766,214
• Time in Patent Office: 1,005 Days
• Field of Search: 264/4.1, 264/4.3, 428/402.2, 424/33, 424/455, 424/460, 424/461
• US Class Current: 264/4.3
• CPC Class Codes: A61K 9/1652 Polysaccharides, e.g. alginate, cellulose derivatives; Cyclodextrin homeopathic globules A61K9/1623
• CPC Class Codes: A61K 9/5036 Polysaccharides, e.g. gums, alginate; Cyclodextrin
• CPC Class Codes: A61K 9/5089 Processes
• CPC Class Codes: Y10T 428/2984 Microcapsule with fluid core [includes liposome]

Abstract

Capsules are formed herein a liquified core while avoiding capsule core gelation by adding drops of a solution of either an anionic polymer composition or a cationic polymer composition to a solution of an ionic polymer of opposite charge. The drops contain an active ingredient such as a cell or microorganism capable of producing a biologically active product or can contain a biological or chemical composition. The interface of the ionic polymers form a permeable membrane surrounding the liquid drops.

Claims

1. A process for producing a capsule having an aqueous liquid core from ionic polymers while avoiding gelation of the core comprising the steps of:

forming an aqueous non-gel liquid droplet from an aqueous solution of a first ionic polymer selected from the group consisting of at least one anionic polymer and at least one cationic polymer, said liquid droplet containing an active material suspended in said droplet; and

contacting said aqueous non-gel droplet with an aqueous solution of at least one second ionic polymer, said second ionic polymer having an ionic charge opposite from said first ionic polymer whereby said first and second polymers interact with each other and wherein said first ionic polymer and said second ionic polymer are free to react with each other along the entire chain lengths of said polymers where said polymers come into reactive contact with each other to form a membrane encapsulating said non gel droplets and said active material or aqueous liquid core containing said active material, and said encapsulated non gel droplets being suspended in said aqueous solution of said second ionic polymer.

2. The process of claim 1 wherein said active material is a lymphocyte.

3. The process of claim 1 wherein said active material is a bacteria.

4. The process of claim 1 wherein said active material is a biologically active compound.

5. The process of claim 4 wherein said compound is an enzyme.

6. The process of claim 4 wherein said compound is a hormone.

7. The process of claim 1 wherein said active material is at least one living cell.

8. The process of claim 7 wherein said cell is a hybridoma.

9. The process of claim 1 wherein the said active material is a microorganism.

10. The process for producing a product for a living cell which comprises encapsulating said cell by the process of claim 1 and controlling the permeability of said membrane to prevent said product from permeating said membrane, collecting said encapsulated cell, rupturing said membrane and recovering said product.

11. The process of claim 10 wherein said cell is a hybridomia.

12. The process of claim 10 wherein said cell is a lymphocyte cell.

13. The process of any one of claims 1, 10, 11 or 12 wherein said anionic polymer is selected from the group consisting of alginate and carragenan.

14. The process of anyone of claims 1, 10, 11 or 12 wherein said cations polymer is selected from the group consisting of chitosan and polylysine.