Hematocrit measuring apparatus

• US 4,745,279 A
• Filed: 01/02/1986
• Issued: 05/17/1988
• Est. Priority Date: 01/02/1986
• Status: Expired due to Fees

• Current Assignee: Baxter International Inc.
• Original Assignee: American Hospital Supply Corp. (Baxter International Inc.)
• Inventors: Heinemann, Stan O., Karkar, Maurice N., Long, Warren M.
• Primary Examiner(s): Howell, Janice A.
• Assistant Examiner(s): Hanig, Richard E.
• Application Number:
  US06/815,576
• Time in Patent Office: 866 Days
• Field of Search: 250/343, 356/39, 356/41, 356/40, 356/446, 356/448, 356/411, 422/44, 422/45, 128/633, 128/634, 128/632
• US Class Current: 250/343
• CPC Class Codes: G01N 2015/055 for hematocrite determination
• CPC Class Codes: G01N 2021/0367 Supports of cells, e.g. pivotable
• CPC Class Codes: G01N 21/05 Flow-through cuvettes G01N21/09 takes precedence; handling fluid samples G01N1/10
• CPC Class Codes: G01N 21/35 using infrared light G01N21/39 takes precedence
• CPC Class Codes: G01N 21/53 within a flowing fluid, e.g. smoke
• CPC Class Codes: G01N 21/85 Investigating moving fluids or granular solids
• CPC Class Codes: G01N 2201/0621 Supply

Abstract

The hematocrit of a volume of blood can be determined in real time without volume or flow rate limitations by sensing the diffusion of infrared light by the bloodstream. For this purpose, an infrared light beam is directed at a transparent cuvette through which the blood is conveyed, and a photodetector is mounted beside the cuvette at an angle such that the intersection of the light beam and of the principal sensitivity lobe of the detector is wholly spaced from the multiple scatter zone adjacent to the light source. The hematocrit signal produced by the photodetector can be corrected for the effect of oxygen saturation by providing an oxygen saturation signal, multiplying the hematocrit signal by a function of the oxygen saturation signal and using a look-up table to derive a linear true hematocrit signal. Means are also provided to compensate the detected hematocrit signal for the effects of temperature, drive current, and aging.

Claims

1. Apparatus for measuring the hematocrit of a flowing bloodstream in real time, comprising:

(a) transparent cuvette means for containing said flowing bloodstream;

(b) infrared light source means adjacent said cuvette means for illuminating said bloodstream;

(c) detector means adjacent said cuvette means for detecting said infrared light after diffusion thereof by said bloodstream; and

(d) signal-producing means for producing a hematocrit signal which is a predetermined function of said detected diffused light;

(e) second detector means for compensating said first-named detector means; and

(f) second light source means for directly illuminating the said second detector means, said second light source and said first-named light source being connected in series;

(g) said signal-producing means being arranged to produce a hematocrit signal which is a function of the ratio of the light values senses by said first-named and second detector means, respectively.

2. The apparatus of claim 1, further comprising means for maintaining said first and second detector means at substantially the same temperature.

3. Apparatus for measuring the hematocrit of a flowing bloodstream in real time, comprising:

(a) first means for producing a first hematocrit signal which is a function of the diffusion of an infrared light beam by said bloodstream;

(b) second means for producing a signal representative of the oxygen saturation of said bloodstream;

(c) third means for multiplying said first hematocrit signal by a function of said oxygen saturation signal to produce an adjusted hematocrit signal; and

(d) look-up table means for producing a linear hematocrit signal from predetermine combinations of said first hematocrit and oxygen saturation signal.

4. The apparatus of claim 3, in which said first means include a first light source and a first photodetector positioned to see only light diffused by said bloodstream;

and said second means include a second light source and a second photodetector positioned to see light multiplescattered by said bloodstream.

5. The apparatus of claim 4, in which said first light source and first photodetector are active at different times than said second light source and photodetector.

6. The apparatus of claim 4, further comprising:

(e) a third light source and a third photodetector positioned to see only said third light source;

(f) said first and third light sources being connected in series, and said first hematocrit signal being a function of the ratio of the light values detected by said first and third photodetectors.

7. Apparatus for measuring the hematocrit of a flowing bloodstream in real time, comprising:

(a) a cuvette adapted to have a bloodstream flowing therethrough, said cuvette having a longitudinal axis;

(b) a holder for receiving said cuvette;

(c) first light source and photodetector means mounted, respectively, in said holder so as to detect only diffused light;

(d) second light source and photodetector means mounted in said holder so as to detect multiple-scattered light;

(e) third light source and photodetector means mounted in said holder in a position to only see each other; and

(f) resilient, transparent, thermally conductive encapsulation means for providing a seat for positioning said cuvette in said holder, said encapsulation means enclosing all of said light source and photodetector means and maintaining them at substantially the same temperature.

8. The apparatus of claim 7, in which said cuvette has a substantially flat side surface, said encapsulation means have an inclined side surface adapted to mate with said flat surface, and said first photodetector means are positioned within said inclined surface.

9. The apparatus of claim 8, in which said cuvette further has a substantially flat bottom surface, said encapsulation means have a substantially horizontal surface adapted to mate with said flat bottom surface, and said second light source means photodetector means are positioned within said horizontal surface.

10. The apparatus of claim 9, in which said first light source means are also positioned within said horizontal surface.

11. The apparatus of claim 7, in which said holder has a wall including slots, said cuvette includes matching tabs, and said slots and tabs cooperate to hold said cuvette against rotation in said holder.

12. The apparatus of claim 7, in which said encapsulation means have a horizontal surface including an elevated portion for contacting said cuvette immediately above said first and second light source and said second photodetector means.

13. A cuvette for use in determining the hematocrit of a flowing bloodstream as a function of oxygen saturation, comprising:

(a) a substantially cylindrical body having a central portion with a plurality of longitudinally extending flat sides forming an acute angle with each other, and

(b) an opaque cover fixed with respect to said body on the side opposite said flat sides.

14. The cuvette of claim 13, in which said cover is generally U-shaped and carries positioning tabs on the legs of said U for longitudinal positioning of said cuvette within a probe.

15. The cuvette of claim 14, in which one leg of said U carries a different number of said positioning tabs than the other leg.

16. Apparatus for measuring the hematocrit of a flowing bloodstram in real time, comprising:

(a) transparent cuvette means for containing said flowing bloodstream;

(b) infrared light source means adjacent said cuvette means for illuminating said bloodstream;

(c) detector means adjacent said cuvette means for detecting said infrared light after diffusion thereof by said bloodstream;

(d) signal-producing means for producing a hematocrit signal which is a predetermined function of said detected diffused light; and

(e) said infrared light source means being arranged to emit a beam of light, said detector means having a restricted field of vision, and the intersection of said beam and field being sufficiently deep within said bloodstream to cause said detector means to see substantially only light diffused by said bloodstream.

17. The apparatus of claim 16, in which said intersection is substantially spaced from the multiple scatter zone of said bloodstream.

18. A method for measuring the hematocrit of a flowing bloodstream in real time, comprising the steps of:

(a) illuminating said bloodstream with light;

(b) detecting a first portion of said light diffused by said bloodstream;

(c) detecting a second portion of said light multiple-scattered by said bloodstream;

(d) producing an oxygen saturation signal which is a function of said detected multiple-scattered light portion;

(e) producing a first hematocrit signal which is a function of said detected diffused light portion; and

(f) using said oxygen saturation signal to adjust said first hematocrit signal so as to produce a second hematocrit signal which is independent of oxygen saturation.

19. The method of claim 18, in which said adjustment of said first hematocrit signal is a multiplication, and said second hematocrit signal is linearized by the use of a predetermined look-up table.