Encapsulated active material system

• US 4,749,620 A
• Filed: 09/18/1985
• Issued: 06/07/1988
• Est. Priority Date: 02/15/1984
• Status: Expired due to Term

• Current Assignee: Massachusetts Institute of Technology
• Original Assignee: Massachusetts Institute of Technology
• Inventors: Rha, ChoKyun, Rodriguez-Sanchez, Dolores
• Primary Examiner(s): Lovering, Richard D.
• Application Number:
  US06/748,364
• Time in Patent Office: 993 Days
• Field of Search: 264/4.1, 264/4.3, 428/402.2, 424/455, 424/460, 424/461, 424/491, 424/493, 424/93, 514/963
• US Class Current: 428/402.2
• CPC Class Codes: A61K 9/1652 Polysaccharides, e.g. alginate, cellulose derivatives; Cyclodextrin homeopathic globules A61K9/1623
• CPC Class Codes: A61K 9/5036 Polysaccharides, e.g. gums, alginate; Cyclodextrin
• CPC Class Codes: A61K 9/5089 Processes
• CPC Class Codes: Y10T 428/2984 Microcapsule with fluid core [includes liposome]

Abstract

Capsules are formed herein a liquified core while avoiding capsule core gelation by adding drops of a solution of either an anionic polymer composition or a cationic polymer composition to a solution of an ionic polymer of opposite charge. The drops contain an active ingredient such as a cell or microorganism capable of producing a biologically active product or can contain a biological or chemical composition. The interface of the ionic polymers form a permeable membrane surrounding the liquid drops.

Claims

1. A capsule comprising a polymeric membrane surrounding a liquid core wherein said membrane is formed by the interaction of at least one anionic polymer with at least one cationic polymer wherein molecular chains comprising said anionic polymer and said cationic polymer are oriented substantially within said membrane so that said polymers are free to react with each other along the entire chain lengths of said polymers where said polymers come into reactive contact with each other and wherein said cationic polymer forms the inner surface of said membrane adjacent liquid core.

2. The capsule of claim 1 wherein said liquid core contains a living cell.

3. The capsule of claim 2 wherein said cell is hybridoma.

4. The capsule of claim 2 wherein said cell is a lymphocyte cell.

5. The capsule of any one of claims 1, 2, 3 or 4 wherein said anionic polymer is selected from the group consisting of alginate and carragenan.

6. The capsule of any one of claims 1, 2, 3 or 4 wherein said cationic polymer is selected from the group consisting of chitosan and polylysive.

7. The capsule of any one of claims 2, 3 or 4 which includes a second membrane formed by cross-linking the anionic polymer to the anionic polymer portion of said polymeric membrane with a divalent metal ion.

8. The capsule of any one of claims 2, 3 or 4 which includes a second alginate membrane formed by cross-linking the anionic polymer to the anionic polymer portion of said polymeric membrane with a divalent metal ion selected from the group consisting of calcium, barium and mixtures thereof.