Method for filtering Bacillus subtilis

• US 4,751,007 A
• Filed: 09/08/1987
• Issued: 06/14/1988
• Est. Priority Date: 03/27/1987
• Status: Expired due to Term

• Current Assignee: Shinji Iijima, Takeshi Kobayashi, Toshiba Carrier Company Limited (Toshiba Corporation)
• Original Assignee: Shinji Iijima, Takeshi Kobayashi, Toshiba Carrier Company Limited (Toshiba Corporation)
• Inventors: Kobayashi, Takeshi, Iijima, Shinji, Minami, Toshiro, Katoh, Yoshihisa, Yasuda, Shunji
• Primary Examiner(s): Cintins, Ivars
• Application Number:
  US07/093,649
• Time in Patent Office: 280 Days
• Field of Search: 210/767, 210/779, 210/791, 210/797, 210/798, 210/805, 210/490, 210/496, 210/497.01, 210/510.1, 435/261, 435/803, 435/839
• US Class Current: 210/791
• CPC Class Codes: B01D 2311/16 Flow or flux control
• CPC Class Codes: B01D 2315/10 Cross-flow filtration
• CPC Class Codes: B01D 2321/04 Backflushing
• CPC Class Codes: B01D 61/147 Microfiltration
• CPC Class Codes: B01D 65/02 Membrane cleaning or sterilisation ; Membrane regeneration
• CPC Class Codes: B01D 71/024 Oxides
• CPC Class Codes: Y10S 435/803 Physical recovery methods, e.g. chromatography, grinding

Abstract

A method comprising passing a culture solution including Bacillus subtilis or gene-recombinated Bacillus thereof through a pipe-like ceramic filter at a flow rate of 2 m/sec or less, the ceramic filter having a filter layer at an inner cylindrical surface thereof, the filter layer being made of spherical particles, and a surface roughness (Rmax) of the inner surface of the ceramic filter being 10 microns or less.

Claims

1. A method comprising passing a culture solution including Bacillus subtilis or gene-recombinated Bacillus thereof through a pipe-like ceramic filter at a flow rate of 2 m/sec or less, the ceramic filter having a filter layer at an inner cylindrical surface thereof, the filter layer being made of spherical particles, and a surface roughness (Rmax) of the inner surface of the ceramic filter being 10 microns or less.

2. The method of claim 1, wherein the filter layer consists of plural coatings made of alumina powder.

3. The method of claim 1, wherein the culture solution is contained in a container connected to a filter casing in which the ceramic filter is set in such a manner that the culture solution can be repeatedly circulated through the container and the ceramic filter.

4. The method of claim 1, wherein the culture solution is filtered by the ceramic filter in a cross flow manner.

5. The method of claim 4, wherein the ceramic filter is back washed while the culture solution is filtered.