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Methods and kits for performing nucleic acid hybridization assays

  • US 4,751,177 A
  • Filed: 06/13/1985
  • Issued: 06/14/1988
  • Est. Priority Date: 06/13/1985
  • Status: Expired due to Term
First Claim
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1. A method for searching a sample solution for selected target polynucleotide suspended in the solution comprising the steps of:

  • (a) exposing the sample to a mediator polynucleotide having a first portion with a nucleotide sequence complementary to and hybridizable with an immobilized polynucleotide and having a second portion, separate and distinct from the first portion, with a nucleotide sequence complementary to and hybridizable with a first portion of the target polynucleotide;

    (b) introducing to the sample a probe polynucleotide having an attached reporter group and having a portion with a nuceotide sequence complementary to and hybridizable with a second portion of the target polynucleotide, said second portion being separate and distinct from said first portion of the target polynucleotide;

    (c) hybridizing the first portion of the target with the second portion ofthe mediator polynucleotide and the second portion of the target polynucleotide with the portion of the probe polynucleotide; and

    (d) immobilizing the mediator polynucleotide by hybridization of the first portion of the mediator polynucleotide with the immobilized polynucleotide.

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