Rapid assay processor

• US 4,753,775 A
• Filed: 04/12/1985
• Issued: 06/28/1988
• Est. Priority Date: 04/12/1985
• Status: Expired due to Fees

• Current Assignee: Dade Behring, Inc. (Siemens AG)
• Original Assignee: E. I. du Pont de Nemours and Company (Corteva, Inc.)
• Inventors: Ebersole, Richard C., Gelormini, Frank T., Forsythe, Jesse G. Jr.
• Primary Examiner(s): Marcus, Michael S.
• Application Number:
  US06/722,373
• Time in Patent Office: 1,173 Days
• Field of Search: 141/9, 141/100, 141/237, 141/241, 141/244, 222/330, 422/63, 422/65, 422/73, 422/100, 422/102, 422/99, 422/81, 422/69, 436/809, 436/810, 436/185
• US Class Current: 422/81
• CPC Class Codes: B01L 2300/0636 Integrated biosensor, microarrays
• CPC Class Codes: B01L 3/021 Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
• CPC Class Codes: B01L 3/508 rigid containers not provided for above
• CPC Class Codes: G01N 2035/1055 for immobilising reagents, e.g. dried reagents
• CPC Class Codes: G01N 33/48 Biological material, e.g. blood, urine G01N33/02, G01N33/26, G01N33/44, G01N33/46 take precedence; Haemocytometers counting blood corpuscules distributed over a surface by scanning the surface G06M11/02
• CPC Class Codes: Y10S 436/808 Automated or kit
• CPC Class Codes: Y10S 436/809 Multifield plates or multicontainer arrays
• CPC Class Codes: Y10T 436/2575 Volumetric liquid transfer

Abstract

A system and method is disclosed for the rapid detection and (or) purification of analytes in a sample. The system makes use of a fluid receptacle containing a capture reagent and a manifold having two ports. A sample containing an analyte disposed within the fluid receptacle is subjected to a capture reagent and processed by inserting the open end of the receptacle in one port. Vacuum, gas, reagents, and wash fluids are then applied to the second port to effect rapid interaction between the capture reagent and analyte, efficient washing of the capture reagent and detection and/or collection of the analyte.

The system and method provide a rapid safe, uncomplicated means of automating processes based on affinity complexation principles. Encompassed within this category of processes are immunoassays, nucleic acid hybridization tests and affinity separations.

Claims

1. A system for processing a sample, containing an analyte to be determined, in a fluid receptacle, the receptacle containing a capture reagent for the analyte, the system comprising:

a manifold, defining a closed chamber, having a first port on an upper portion of the manifold and a second port, both ports communicating with the chamber.

a fluid receptacle having a solid support for the capture reagent, having a single open end and an enlarged bulbous portion opposite the open end, and being adapted to receive the sample, the capture reagent being disposed on the solid support,

means adapted to detachably mount the fluid receptacle above the manifold with the open end below the remainder of the receptacle and in fluid tight communication with the first port, and

means for selectively introducing to and removing from the chamber and hence the receptacle through the second port vacuum, gas, reagents and wash fluids, thereby to alternatively effect rapid interaction between the capture reagent and analyte, efficient washing of the capture reagent, collection of the analyte, and cleaning of the closed chamber.

2. The system of claim 1 in which the introducing means applies coordinated pulses of vacuum, fluids, and air to the chamber to control both the direction of fluid movement within the receptacle and to effect fluid communication between the chamber and the receptacle, and includes means for maintaining the chamber at subatmospheric pressure.

3. The system of claim 2 which includes a plurality of fluid receptacles and wherein the manifold defines plural first ports communicating with the closed chamber, each adapted to detachably receive and to place a different fluid, with the open end below the remainder of the receptacle, in fluid tight communication with the chamber, thereby to simultaneously permit the plural fluid receptacles to be subjected to the vacuum, gas, reagents and/or wash fluids.

4. The system of claim 3 in which the plural first ports are located at equal distances from the second port.

5. The system of claim 1 which includes a plurality of fluid receptacles and wherein the manifold defines plural first ports communicating with the closed chamber, each adapted to detachably receive and to place a different fluid receptacle, with the open end below the remainder of the receptacle, in fluid tight communication with the chamber, thereby to simultaneously permit the plural fluid receptacles to be subjected to the vacuum, gas, reagents and/or wash fluids.

6. The system of claim 5 in which the plural first ports are located at equal distances from the second port.

7. The system of claim 2 which includes means to collect waste fluids disposed between the second port and the vacuum means.

8. The system of claim 2 wherein the fluid receptacle bulbous portion is closed defining a compartment and has a porous retainer in the open end, the capture reagent being disposed in the compartment.

9. The system of claim 8 wherein the solid support is in the form of particulates that cannot pass through the retainer.

10. The system of claim 8 wherein the solid support is the porous retainer.

11. The system of claim 1 wherein the fluid receptacle bulbous portion is closed defining a compartment, and has a porous retainer and the solid support positioned in the open end.

12. The system of claim 11 wherein the fluid receptacle compartment is collapsible.

13. The system of claim 11 wherein the closed chamber is in the form of an elongated passageway with the second port disposed at one end of the passageway and which includes a third port disposed at the other end of the passageway.

14. A test apparatus for processing samples, the test apparatus comprising:

a fluid receptacle having a single access port and a solid support, a capture reagent disposed on the solid support, an enlarged bulbous portion opposite the access port, and adapted to contain a sample having an analyte to be processed,

a closed manifold having a first port on an upper portion of the manifold adapted to detachably receive the access port of the receptacle and place it in fluid tight communication with the manifold and a second port, the receptacle being positioned above the manifold, with the access port being positioned in the first port, the second port being located below the first port,

means to selectively subject the manifold through the second port to vacuum, gas, reagents, and wash fluids,

the receptable forming with the manifold a closed system, whereby the sample can be selectively subjected to the vacuum, gas, reagents and wash fluids.

15. The apparatus set forth in claim 14 which includes a plurality of the receptacles and in which the manifold has a plurality of first ports each adapted to detachably a different receptacle and place it in fluid tight communication with the manifold.

16. The apparatus set forth in claim 15 in which the first manifold ports are located at equal distances from the second port.

17. The apparatus of claim 16 wherein each fluid receptacle bulbous portion is closed defining a compartment, and has a porous retainer and the solid support positioned in the open end.

18. The apparatus of claim 17 wherein the selective subjecting means includes a separate valve for coupling sources of each of the vacuum, gas, reagents and wash fluids with the manifold, and a controller for selectively actuating the valves in concert to introduce and withdraw the air, vacuum, wash fluids and reagents into and from the receptacles.

19. The apparatus of claim 15 wherein each fluid receptacle bulbous portion is closed defining a compartment, and has a porous retainer positioned between the access port and the bulbous portion.

20. The apparatus of claim 19 wherein the selective subjecting means includes a separate valve for coupling sources of each of the vacuum, gas, reagents and wash fluids with the manifold, and a controller for selectively actuating the valves in concert to introduce and withdraw the vacuum, air, wash fluids and reagents into and from the receptacles.