Method for the production of sorbitol and gluconate
First Claim
1. A method for the production of sorbitol and gluconolactone comprising reacting glucose and fructose in the presence of a strain of Zymomonas mobilis which contains the enzyme complex glucose/fructose transhydrogenase including the tightly bound co-factor(s), or a cell-free extract of such a strain of Zymomonas mobilis, containing the said enzyme complex, in which the strain of Zymomonas mobilis, the cell-free extract or the reaction conditions are such that the further reaction of gluconolactone is substantially prevented.
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Abstract
A method for the simultaneous production of sorbitol and either gluconolactone or gluconate from glucose and fructose. To this end to produce sorbitol and gluconolactone, glucose and fructose are reacted in the presence of a cell or cell-free extract of a bacteria, which contains the enzyme complex glucose/fructose transhydrogenase including the tightly bound co-factor(s), under condition whereby the further metabolism of gluconolactone is substantially prevented. In order to produce sorbitol and gluconate glucose and fructose are reacted in the presence of a cell or cell-free extract of a bacteria which contains an enzyme system comprising the enzyme complex glucose/fructose transhydrogenase including the tightly bound co-factor(s) and the enzyme gluconolactonase. This reaction is carried out under conditions whereby the metabolism of gluconate is substantially prevented. Preferably the bacteria used in this process is of the genus Zymomonas and most preferably of the species Zymomonas mobilis. The enzyme complex referred to as glucose/fructose transhydrogenase including the tightly bound co-factor(s) has been isolated in its pure form.
71 Citations
21 Claims
- 1. A method for the production of sorbitol and gluconolactone comprising reacting glucose and fructose in the presence of a strain of Zymomonas mobilis which contains the enzyme complex glucose/fructose transhydrogenase including the tightly bound co-factor(s), or a cell-free extract of such a strain of Zymomonas mobilis, containing the said enzyme complex, in which the strain of Zymomonas mobilis, the cell-free extract or the reaction conditions are such that the further reaction of gluconolactone is substantially prevented.
- 2. A method for the production of sorbitol and gluconate comprising reacting glucose and fructose in the presence of a strain of Zymomonas mobilis which contains an enzyme system comprising the enzyme complex glucose/fructose transhydrogenase including the tightly bound cofactor(s) and the enzyme gluconolactonase, or a cell-free extract of such a strain of Zymomonas mobilis containing the said enzyme system, in which the strain of Zymomonas mobilis, the cell-free extract or the reaction conditions all such that the further reaction of gluconate is substantially prevented.
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14. A method for the production of sorbitol and gluconolactone comprising reacting glucose and fructose in the presence of Zymomonas mobilis cells which have been washed in a phosphate-free buffer and which contain the enzyme complex glucose/fructose transhydrogenase including tightly bound co-factor(s) or in the presence of cell-free extract of such Zymomonas mobilis cells to thereby prevent further reaction of gluconolactone.
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15. A method for the production of sorbitol and gluconate comprising reacting glucose and fructose in the presence of Zymomonas mobilis cells, which have been washed in a phosphate-free buffer and which contain the enzyme complex glucose/fructose transhydrogenase including tightly bound co-factor(s) and the enzyme gluconolactonase or in the presence of cell-free extract of such Zymomonas mobilis cells to thereby prevent further reaction of gluconate.
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16. A method for the production of sorbitol and gluconolactone comprising reacting glucose and fructose in the presence of Zymomonas mobilis cells which contain the enzyme complex glucose/fructose transhydrogenase including tightly bound co-factor(s) and which are phenotypically unable to metabolize gluconolactone.
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17. A method for the production of sorbitol and gluconate comprising reacting glucose and fructose in the presence of Zymomonas mobilis cells which contain the enzyme complex glucose/fructose transhydrogenase including tightly bound co-factor(s) and the enzyme gluconolactonase and which are phenotypically unable to metabolize gluconate.
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20. A method for the production of sorbitol and gluconolactone comprising reacting glucose and fructose in the presence of the enzyme complex glucose/fructose transhydrogenase including the tightly bound co-factor(s) isolated from a strain of Zymomonas mobilis.
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21. A method for the production of sorbitol and gluconate comprising reacting glucose and fructose in the presence of the enzyme complex glucose/fructose transhydrogenase including the tightly bound co-factor(s) and the enzyme gluconolactonase, both the enzyme complex and the gluconolactonase being isolated from a strain of Zymomonas mobilis.
Specification