Method and apparatus for sorting particles

• US 4,756,427 A
• Filed: 10/14/1987
• Issued: 07/12/1988
• Est. Priority Date: 09/11/1984
• Status: Expired due to Fees

• Current Assignee: Agrogen Foundation, K-Midt Ltd. Liab., Co.
• Original Assignee: Partec AG
• Inventors: Schumann, Johannes, Gohde, Hildegard
• Primary Examiner(s): Reeves, Robert B.
• Assistant Examiner(s): NOT, DEFINED
• Application Number:
  US07/110,032
• Time in Patent Office: 272 Days
• Field of Search: 209/3.1, 209/552, 209/606, 209/643, 209/906, 209/932, 209/644, 356/39, 356/335, 324/71.4, 250/222.2, 406/168, 406/183, 406/181, 406/175, 406/197
• US Class Current: 209/3.1
• CPC Class Codes: B01L 2200/0652 Sorting or classification of particles or molecules
• CPC Class Codes: B01L 2400/0439 ultrasonic vibrations, vibrating piezo elements
• CPC Class Codes: B01L 2400/0478 pistons
• CPC Class Codes: B01L 2400/0487 fluid pressure, pneumatics
• CPC Class Codes: B01L 3/502761 specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules investigating characteristics of particles G01N15/00
• CPC Class Codes: G01N 15/1404 Handling flow, e.g. hydrodynamic focusing
• CPC Class Codes: G01N 15/1484 microstructural devices
• CPC Class Codes: G01N 15/149 specially adapted for sorting particles, e.g. by their size or optical properties
• CPC Class Codes: G01N 2015/1415 Control of particle position
• CPC Class Codes: Y10S 209/906 Pneumatic or liquid stream feeding item

Abstract

A sorting method is disclosed in which particles suspended in a fluid are conducted in a closed duct and pass a measurement location in which particles to be selected trigger a signal by a sensing device. At a downstream fork, a pressure wave generated in response to the signal diverts the stream containing the particles from one branch to the other. An apparatus for carrying out this method is disclosed and includes a supply duct for the particle stream, a measurement duct, a measurement position in the measurement duct, a fork downstream of the measurement duct leading to a sorting branch duct and waste branch duct, and a pressure wave generator which is disposed in one of the ducts leading away from the fork.

Claims

1. A method of sorting small particles including the steps of

providing a closed duct having an inlet and a fork at which the duct separates into two branch ducts;

conducting a stream of liquid into the duct inlet with a stream of particles suspended therein, the particle stream normally flowing through a first one of the branch ducts;

surrounding the stream of liquid with a particle-free enveloping current of liquid to produce substantially laminar flow;

providing a measurement station along the closed duct upstream of the fork for sensing a predetermined property of particles in the stream and for producing a signal when the property is sensed; and

momentarily altering the volume of a portion of one of said branch ducts to create a pressure disturbance downstream of the fork in response to the signal indicating sensing of the predetermined property in a specific particle at the measurement station without introduction of gas so that flow into the first branch duct is momentarily disturbed while the specific particle is at the fork while both branch ducts remain open to liquid flow, thereby interrupting particle stream flow into the first branch duct and causing the specific particle to flow into the second branch duct without eliminating the laminar flow.

2. A method according to claim 1 wherein the disturbance comprises a pressure wave generated in the first branch duct downstream of the fork, the wave causing particle stream flow to be momentarily diverted from the first branch duct to the second branch duct.

3. A method according to claim 1 wherein the disturbance comprises a pressure wave generated in the second branch duct downstream of the fork.

4. An apparatus for sorting particles comprising

a fluid duct having a measurement region with an inlet and a fork at which said duct separates into two branch ducts;

first sensing means along said measurement region for sensing a predetermined characteristic of particles flowing therein and for producing a sensing signal in response to the sensed characteristic;

means for supplying a liquid to said inlet with a stream of particles suspended in said liquid so that said stream of particles normally flows into a first one of said branch ducts;

means for supplying a sheating liquid to said fluid duct to form a particle-free sheath surrounding said stream of particles; and

means along one of said branch ducts downstream of said fork, for momentarily altering the volume of said one of said ducts in response to said sensing signal for generating a pressure wave and for temporarily diverting a segment of said particle stream into the second one of said branch ducts while both of said branch ducts remain open to liquid flow.

5. An apparatus according to claim 4 wherein said means for generating said pressure wave is located along said first one of said branch ducts.

6. An apparatus according to claim 5 and further comprising second sensing means located along said second one of said branch ducts for monitoring the particle sorting.

7. An apparatus according to claim 6 wherein said means for generating a pressure wave comprises a piezoelectric column coupled to said branch duct.

8. An apparatus according to claim 7 wherein said means for generating is located outside of said branch duct.

9. An apparatus according to claim 6 wherein said means for generating a pressure wave comprises a stepping motor attached to said branch duct.

10. An apparatus according to claim 9 wherein said means for generating is located outside of said branch duct.

11. An apparatus according to claim 5 wherein said means for generating a pressure wave comprises a piezoelectric column coupled to said branch duct.

12. An apparatus according to claim 5 wherein said means for generating a pressure wave comprises a stepping motor attached to said branch duct.

13. An apparatus according to claim 4 wherein said means for generating a pressure wave comprises a piezoelectric column coupled to said branch duct.

14. An apparatus according to claim 4 wherein said means for generating a pressure wave comprises a stepping motor attached to said branch duct.