Chromatographic devices having modified edges

• US 4,757,004 A
• Filed: 03/16/1984
• Issued: 07/12/1988
• Est. Priority Date: 03/16/1984
• Status: Expired due to Term

• Current Assignee: Dade Behring Marburg GmbH (Siemens AG)
• Original Assignee: Syntec Incorporated (Atos SE)
• Inventors: Ullman, Edwin F., Houts, Thomas M.
• Primary Examiner(s): Nucker, Christine M.
• Application Number:
  US06/591,155
• Time in Patent Office: 1,579 Days
• Field of Search: 435/7, 435/803, 435/805, 436/528, 436/161, 436/162, 436/169, 436/810, 436/824, 422/56, 422/70, 422/71, 210/456, 210/190.2, 162/194, 162/286, 162/260, 427/3, 219/121 LG, 219/121 LN, 219/121 LQ, 219/121 LW
• US Class Current: 435/7.91
• CPC Class Codes: G01N 30/90 Plate chromatography, e.g. thin layer or paper chromatography
• CPC Class Codes: G01N 30/92 Construction of the plate
• CPC Class Codes: Y10S 435/803 Physical recovery methods, e.g. chromatography, grinding
• CPC Class Codes: Y10S 435/805 Test papers
• CPC Class Codes: Y10S 436/81 Tube, bottle, or dipstick

Abstract

A device is disclosed for use in a chromatographic system wherein a component of a mixture is paritioned between a liquid phase and an immobile phase. The device is comprised of a chromatographic material. In the chromatographic system the component traverses at least a portion of the chromatographic material. The device generally has at least one longitudinal edge substantially corresponding to the direction of traverse of the component. The longitudinal edge includes means for controlling the shape of the front of the traversing component.

Claims

1. A device for use in a chromatographic system wherein a component of a mixture is partitioned between a liquid phase and an immobile phase, said device being comprised of a chromatographic material having a longitudinal edge, wherein in said system said component traverses at least a portion of said chromatographic material and said longitudinal edge includes means for controlling the shape of the front of the traversing component.

2. The device of claim 1 wherein said means render the shape of said front substantially flat or convex, when considered perpendicular to the direction of traversal of said component.

5. The device of claim 1 wherein the chromatographic material is a strip of bibulous material.

6. The device of claim 1 wherein a member of a specific binding pair is non-diffusively bound to the chromatographic material.

7. An immunochromatographic device comprising the device of claim 6 wherein the member of the specific binding pair is an antigen or an antibody.

8. The immunochromatographic device of claim 7 wherein a member of a signal producing system is bound to said chromatographic material.

9. The immunochromatographic device of claim 7 wherein the member of a signal producing system is an enzyme.

10. The immunochromatographic device of claim 6 wherein the signal producing system comprises two enzymes which are related by the product of one enzyme being a substrate for the other enzyme.

18. A diagnostic kit, for conducting an immunochromatographic assay, which comprises in packaged combination

(a) the device of claim 1, and

(b) a member of said specific binding pair conjugated to a member of a signal producing system.

3. A device for use in a chromatographic system wherein a component of a mixture is partitioned between a liquid phase and an immobile phase, said device being comprised of a chromatographic material having a longitudinal edge, wherein in said system said component traverses at least a portion of said chromatographic material and said longitudinal edge includes means comprising a plurality of indentations for controlling the shape of the front of the traversing component.

4. A device for use in a chromatographic system wherein a component of a mixture is partitioned between a liquid phase and an immobile phase, said device being comprised of a chromatographic material having a longitudinal edge, wherein in said system said component traverses at least a portion of said chromatographic material and said longitudinal edge includes means comprising a chemical substance capable of reducing the rate of migration of said traversing component along said edge for controlling the shape of the front of the traversing component.

11. A device for use in a chromatographic system wherein a component of a mixture is partitioned between a liquid phase and an immobile phase, said device being comprised of a chromatographic material and having a plurality of indentations along a longitudinal edge, the number, dimensions, and configuration of said indentations being sufficient to render the front of said traversing component substantially flat or convex.

12. The device of claim 11 wherein the indentations have an amplitude which affects the configuration of the front in that the greater the amplitude, the less the migration of a traversing component near a longitudinal edge relative to migration away from the edge.

13. The device of claim 12 wherein the strip has a thickness of from about 0.1 to 2 mm and a width of from about 3 to 30 mm and the amplitude of the indentations is from about 0.3 to 10 mm.

14. The device of claim 11 wherein a member of a specific binding pair is non-diffusively bound to the chromatographic material.

15. The device of claim 14 wherein a member of a signal producing system is bound to the chromatographic material.

16. The device of claim 15 wherein the member of the signal producing system is an enzyme.

17. The device of claim 15 wherein the signal producing system comprises two enzymes which are related by the product of one enzyme being a substrate for the other enzyme.

19. A device for for use in a chromatographic system wherein a component in a liquid medium traverses at least a portion of said device, said device comprising at least one strip of a porous material, said strip having two longitudinal edges, said longitudinal edges having a plurality of indentations, the number, configuration, and dimensions of said indentations being sufficient to render the configuration of the front of said traversing component substantially flat or convex.

20. The device of claim 19 wherein the indentations are triangular or semi-circular in shape and have an amplitude which affects the configuration of the front in that the greater the amplitude, the less the migration of the traversing component near the longitudinal edge relative to migration away from the edge.