Compositions and methods for functionalizing nucleic acids

• US 4,762,779 A
• Filed: 06/13/1985
• Issued: 08/09/1988
• Est. Priority Date: 06/13/1985
• Status: Expired due to Term

• Current Assignee: Raylo Chemicals, Inc. (Gilead Sciences Inc.)
• Original Assignee: Amgen Inc.
• Inventors: Snitman, David L.
• Primary Examiner(s): CRANE, LAWRENCE E
• Assistant Examiner(s): Spiegel, Jack
• Application Number:
  US06/744,508
• Time in Patent Office: 1,153 Days
• Field of Search: 435/6, 435/91, 536/27, 536/28, 536/29
• US Class Current: 435/6.12
• CPC Class Codes: C07H 21/00 Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids

Abstract

A composition and a method for 5'"'"'-labelling polynucleotides undergoing solid phase synthesis wherein a phosphoramidite of an ω-hydroxylamine is condensed to a support-bound polynucleotide.

Claims

1. An end-label for attachment to a nucleic acid during solid phase synthesis comprising a phosphoramidite of a hydroxylamine condensed to a polynucleoside.

2. The label according to claim 1 wherein said hydroxylamine contains from 1 to 10 carbon atoms.

3. The label according to claim 2 wherein said hydroxylamine is selected from the group comprising ethanolamine, propanolamine, butanolamine, pentanolamine, hexanolamine, heptanolamine, octanolamine, nonanolamine, decanolamine, and p-aminophenol.

4. A method of attaching an end-label to a nucleic acid during solid phase synthesis comprising the steps of:

preparing a phosphoramidite of a hydroxylamine; and

condensing the phosphoramidite of a hydroxylamine to a support-bound polynucleoside.