Reagent handling

• US 4,764,342 A
• Filed: 02/27/1985
• Issued: 08/16/1988
• Est. Priority Date: 02/27/1985
• Status: Expired due to Fees

• Current Assignee: Il Holding SpA (Werfen SA)
• Original Assignee: Fisher Scientific Co. (Thermo Fisher Scientific Incorporated)
• Inventors: Kelln, Norman G., Tiffany, Thomas O.
• Primary Examiner(s): Marcus, Michael S.
• Application Number:
  US06/706,074
• Time in Patent Office: 1,266 Days
• Field of Search: 422/63, 422/64, 422/65, 422/72, 422/100, 422/102, 436/45, 436/47, 436/166, 436/180, 141/130, 141/5, 141/325, 141/326, 356/246, 356/428, 206/438, 206/508, 206/509, 222/585, 222/588, 220/DIG. 13, 220/368
• US Class Current: 422/72
• CPC Class Codes: B01L 3/508 rigid containers not provided for above
• CPC Class Codes: G01N 2035/00326 Analysers with modular structure
• CPC Class Codes: G01N 2035/00673 of reagents
• CPC Class Codes: G01N 2035/00752 bar codes
• CPC Class Codes: G01N 2035/041 lifting items out of a rack for access
• CPC Class Codes: G01N 2035/0443 for reagents
• CPC Class Codes: G01N 2035/0446 Combinations of the above
• CPC Class Codes: G01N 2035/0448 composed of interchangeable ring elements
• CPC Class Codes: G01N 2035/0451 composed of interchangeable sectors
• CPC Class Codes: G01N 2035/0453 Multiple carousels working in parallel
• CPC Class Codes: G01N 21/07 Centrifugal type cuvettes G01N21/09 takes precedence
• CPC Class Codes: G01N 35/00663 of consumables
• CPC Class Codes: G01N 35/025 having a carousel or turntable for reaction cells or cuvettes
• CPC Class Codes: G01N 35/1002 Reagent dispensers
• CPC Class Codes: G01N 35/1079 with means for piercing stoppers or septums
• CPC Class Codes: Y10T 436/111666 Utilizing a centrifuge or compartmented rotor
• CPC Class Codes: Y10T 436/2575 Volumetric liquid transfer

Abstract

A sealed reagent container structure that contains reagent material, has a body portion with length and width dimensions each greater than its height dimension and port defining neck structure upstanding from the upper wall of the body portion and asymmetrically located so that it is close to one endwall of the body portion. Coupling structure on the bottom wall of the body portion is provided for interengagement with a transport mechanism so that a series of reagent containers may be disposed on the transport mechanism for movement past a reagent station where the reagent material is transferred from the reagent container to the analysis cuvettes for mixing with sample material and analysis.

Claims

1. Sealed reagent container formed of stretched oriented polymeric material comprising a body portion that has length and width dimensions each greater than its height dimension, said body portion having generally vertical sidewall portions extending between a top wall and a bottom wall, said sidewall portions being disposed in converging relation and joining at a nose portion and an end wall disposed at an end of said container opposite said nose portion, said end wall extending between said sidewall portions and said top and bottom walls, reagent material contained in said body portion, a port-defining neck portion formed in said top wall of said body portion and upstanding from the top wall of said body portion, said port-defining neck portion being asymmetrically located so that it is close to the endwall of said body portion remote from said nose portion, coupling structure on the bottom wall of said body portion positioned and arranged for interengagement with a transport mechanism so that a series of said reagent containers may be disposed on such a transport mechanism for movement past a reagent station where the reagent material is transferred from the reagent container to analysis cuvettes for mixing with sample material an analysis;

structure defining a through channel spaced from but adjacent to said port defining neck portion, said through-channel defining structure being open at it top and at its bottom and being sealed to and extending between the top and bottom walls of said body portion; and

means for sealing said port.

2. The container of claim 1 wherein said reagent container coupling structure includes a projection that depends from the bottom wall and said projection carries a machine readable code that is correlated with said reagent material.

3. The container of claim 1 wherein said means for sealing said port comprises a probe puncturable membrane positioned and arranged across said neck portion, and further including removable closure cap structure releasably engaged with said neck portion.

4. The container of claim 1 wherein said top wall includes a portion defining an air chamber structure extending above a junction between said body and neck portions located adjacent said nose portion, the volume of said air chamber being a least five percent of the total volume of the sealed reagent container.

5. The container of claim 1 wherein the quantity of reagent material in said container is sufficient for at least one hundred analyses, the body portion of said container has a sump portion formed in the bottom wall aligned with said neck portion for maximizing transfer efficiency of reagent from said container, and a bottom external surface of said sump portion carries a machine readable code that is correlated with said reagent material.

6. The container of claim 5 wherein said means for sealing said port comprises a probe puncturable membrane, and further including removable closure cap structure releasably engaged with said neck portion.

7. A reagent handling system for use with a clinical analysis system comprising:

a cuvette loading station and sample and reagent supply stations disposed along a straight line path;

transfer structure that includes two spaced apart liquid handling probes fixedly mounted thereon and aligned with said straight line path, means for moving said transfer structure along said straight line path between a first transfer location in said cuvette loading station, a second transfer location in said sample supply station and a third transfer location in said reagent supply station;

a centrifugal analyzer rotor positioned and arranged in said cuvette loading station and having a plurality of analysis cuvettes arranged in annular array therein, each said analysis cuvette having two loading ports spaced a distance corresponding to the spacing of the two probes of said transfer structure, and indexing mechanism at said cuvette loading station positioned and arranged for rotating the analyzer rotor to sequentially position said cuvettes at said first transfer location in the cuvette loading station for loading sample and reagent material from said sample and reagent supply stations, respectively, into said cuvettes under instructions from a system controller;

said sample station including sample container turntable means for selectively positioning a sequence of sample containers in said second transfer location;

said reagent station including reagent container turntable means coaxial with said sample container turntable means for selectively positioning a sequence of reagent containers in said third transfer location;

means sfor separately driving said sample container turntable means and said reagent container turntable means for independently positioning different sample and reagent containers in said second transfer location and said third transfer location, respectively, said reagent container turntable means including a support surface with container coupling means, and

a plurality of reagent containers on said reagent container turntable mechanism,

each said reagent container having a body portion with length and width dimensions each greater than the height dimension of the container, the body portion of each said container having generally vertical sidewall portions extending between a top wall and a bottom wall, said sidewall portions being disposed in converging relation and joining at a nose portion, an end wall being disposed at an end of said container opposite said nose portion and extending between said sidewall portions and said top and bottom walls, a port-defining neck portion formed in said top wall of said body portion and upstanding from the top wall of each said reagent container, said port-defining neck portion being asymmetrically located adjacent that reagent container sidewall which is remote from said nose portion and the bottom wall of each said reagent container including a coupling structure that interengages said container coupling means of said support surface of said reagent container turntable means for secure positioning on said reagent container turntable means, the body portion of each said container having structure defining a through-channel spaced from said port-defining neck portion a distance corresponding to the spacing of said probes, said through-channel defining structure being open at its top and at it bottom and being sealed to and extending between the top and bottom walls of said body portion,

said plurality of reagent containers being compactly arranged in a circumferential array on said support surface of reagent container turntable means for indexing relative to said third transfer location of said reagent loading station, said sample container turntable means holding a series of sample containers in a ring coaxial with said reagent container turntable means,

said transfer structure including a drive means for concurrently inserting tips of said two liquid handling probes into sample and reagent containers located at said second and third transfer locations, respectively, and concurrently withdrawing sample and reagent material therefrom for transfer to an analysis cuvette at said first transfer location in said cuvette loading station.

8. The system of claim 7 wherein said reagent container turntable means includes a series of apertures formed therein and said system further includes a code reader aligned with said apertures, and each said reagent container has a projection that depends from its bottom wall for disposal in a said aperture and carries a machine readable code that is correlated with contents of the container and positioned and arranged to be read by said code reader.

9. The system of claim 8 wherein each said reagent container is formed of stretched oriented polymeric material and the quantity of reagent material in said reagent container is sufficient for at least one hundred analyses.

10. The system of claim 9 wherein the top wall of said reagent container includes a portion defining an air chamber structure extending above the junction between said body and neck portions, a volume of said air chamber being at least five percent of the total volume of the reagent container.