Solubilization of proteins for pharmaceutical compositions using polymer conjugation

• US 4,766,106 A
• Filed: 01/25/1988
• Issued: 08/23/1988
• Est. Priority Date: 06/26/1985
• Status: Expired due to Fees

• Current Assignee: Cetus Oncology Corporation (Novartis Ag)
• Original Assignee: Cetus Corporation (Bayer AG)
• Inventors: Katre, Nandini, Knauf, Michael J.
• Primary Examiner(s): DRAPER, GARNETTE D
• Application Number:
  US07/148,145
• Time in Patent Office: 211 Days
• Field of Search: 424/85, 424/83, 514/12, 514/2, 530/351
• US Class Current: 424/85.2
• CPC Class Codes: A61K 38/2013 IL-2
• CPC Class Codes: A61K 38/215 IFN-beta
• CPC Class Codes: A61K 47/60 the organic macromolecular compound being a polyoxyalkylene oligomer, polymer or dendrimer, e.g. PEG, PPG, PEO or polyglycerol
• CPC Class Codes: A61K 47/6827 Ricin A
• CPC Class Codes: C07K 1/1077 by covalent attachment of residues other than amino acids or peptide residues, e.g. sugars, polyols, fatty acids

Abstract

A pharmaceutical composition is prepared wherein a biologically active conjugated protein which is β-interferon, interleukin-2, or an immunotoxin is dissolved in an aqueous carrier medium without the presence of a solubilizing agent. The unconjugated protein, which is not water-soluble or not readily soluble in water at pH 6-8 without such solubilizing agent, is selectively conjugated to a water-soluble polymer selected from polyethylene glycol homopolymers or polyoxyethylated polyols.

Claims

1. A pharmaceutical preparation comprising a non-toxic, inert, pharmaceutically acceptable aqueous carrier medium in which is dissolved an immunotherapeutic effective amount of biologically active, selectively conjugated, recombinant interleukin-2 protein, wherein the protein is covalently conjugated via up to ten amino acid residues on the protein to a water-soluble polymer selected from the group consisting of polyethylene glycol homopolymers and polyoxyethylated polyols, wherein said homopolymer is unsubstituted or substituted at one end with an alkyl group and said polyol is unsubstituted and wherein said protein in its unconjugated form is normally hydrophobic.

2. The preparation of claim 1 wherein said polymer has a molecular weight of about 300 to 100,000.

3. The preparation of claim 1 wherein said polymer has a molecular weight of 350 to 40,000.

4. The preparation of claim 1 wherein said polymer is conjugated to the protein via the 4-hydroxy-3-nitrobenzene sulfonate ester or the N-hydroxysuccinimide ester of a carboxylic acid of said polymer.

5. The preparation of claim 4 wherein said polymer is an unsubstituted polyethylene glycol homopolymer, a monomethyl polyethylene glycol homopolymer or a polyoxyethylated glycerol.

6. The preparation of claim 1 wherein the medium is at a pH of about 5-8 and contains a buffer.

7. The preparation of claim 1 wherein the medium is at a pH of about 6.5-7.8 and contains a buffer.

8. The preparation of claim 1 wherein the protein is from a human source.

9. The preparation of claim 1 wherein said protein is selectively conjugated via one or two lysine residues on the protein.

10. The preparation of claim 1 wherein said protein is selectively conjugated via three to ten lysine residues on the protein.

11. The preparation of claim 8 wherein the protein is an interleukin-2 mutein.

12. The preparation of claim 11 wherein said mutein has the amino acid(s) which are at position(s) 104 and/or 125 in the native protein substituted with a neutral amino acid.

13. The preparation of claim 12 wherein said mutein is ser₁₂₅ IL-2, des-ala₁ IL-2, des-ala₁ ser₁₂₅ IL-2, des-ala₁ ala₁₀₄ IL-2, or des-ala₁ ala₁₀₄ ser₁₂₅ IL-2.

14. The preparation of claim 1 wherein said protein is selectively conjugated via up to ten lysine residues on the protein.

15. A composition comprising biologically active, normally hydrophobic recombinant interleukin-2 protein that is covalently conjugated via up to ten amino acid residues on the protein to a water-soluble polymer selected from the group consisting of polyethylene glycol homopolymers and polyoxyethylated polyols, wherein said homopolymer is unsubstituted or substituted at one end with an alkyl group and said polyol is unsubstituted.