Protein-free culture medium
First Claim
1. In a protein-free chemically defined tissue culture medium wherein the improvement comprises a combination of a basal medium and a trace element mixture, said trace element mixture comprising a bromine salt, a fluorine salt, and an iodine salt, and, as metal salt other than positive metal ions selected from Group IA and IIA of the Periodic Table of the Elements, said mixture containing only at least one species selected from each member of the group consisting of copper salts, iron salts, zinc salts, manganese salts, silicon salts, molybdenum salts, vanadium salts, nickel salts, tin salts, aluminum salts, silver salts, barium salts, cadminum salts, cobalt salts, chromium salts, germanium salts, rubidium salts, zirconium salts and selenium salts.
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Accused Products
Abstract
A trace element mixture suitable for use in a protein-free tissue culture medium, which comprises water-soluble compounds selected from acids, bases, and salts containing copper, iron, zinc, manganese, silicon, molybdenum, vanadium, nickel, tin, aluminum, silver, barium, bromine, cadmium, cobalt, chromium, fluorine, germanium, iodine, rubidium, zirconium, or selenium, the compounds being devoid of any additional metals other than those present as positive ions selected from groups IA and IIA of the periodic table of elements, wherein the compounds produce a solution containing specified minimum concentrations of the listed elements when dissolved in an amount of water sufficient to produce a concentration for one of the elements equal to the corresponding minimum concentration of the one element while maintaining each remaining element at a concentration equal to or greater than the minimum concentration for the remaining element is disclosed along with cell culture media containing these trace elements and methods of culturing cells using these media.
492 Citations
23 Claims
- 1. In a protein-free chemically defined tissue culture medium wherein the improvement comprises a combination of a basal medium and a trace element mixture, said trace element mixture comprising a bromine salt, a fluorine salt, and an iodine salt, and, as metal salt other than positive metal ions selected from Group IA and IIA of the Periodic Table of the Elements, said mixture containing only at least one species selected from each member of the group consisting of copper salts, iron salts, zinc salts, manganese salts, silicon salts, molybdenum salts, vanadium salts, nickel salts, tin salts, aluminum salts, silver salts, barium salts, cadminum salts, cobalt salts, chromium salts, germanium salts, rubidium salts, zirconium salts and selenium salts.
- 17. In a method of cultivating hybridoma cells in a protein free basal medium, the improvement comprising adding to the basal medium a trace element mixture consisting essentially of copper, iron, zinc, manganese, silicon, molybdenum, banadium, nickel, tin, aluminum, silver, barium, bromine, cadmium, cobalt, chromium, fluorine, germanium, iodine, rubidium, ziroconium, and selenium.
Specification