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Apparatus and method for liquid chromatography

  • US 4,769,153 A
  • Filed: 09/02/1986
  • Issued: 09/06/1988
  • Est. Priority Date: 09/09/1981
  • Status: Expired due to Fees
First Claim
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1. A method of rapidly bringing a chromatographic system to equilibrium pressure after start-up comprising the steps of:

  • setting a pumping speed for pumping fluid from a pumping system into a chromatographic column at a predetermined rate of flow during a chromatographic run;

    starting the chromatographic run, whereby the chromatographic system passes through an unstable period between start and reaching equilibrium pressure;

    measuring, at a time of a low value of pressure near the time of start up, a characteristic of fluid being transferred from the pumping system to the chromatographic column related to the pressure of the fluid and establishing a range of said characteristic between two boundary conditions;

    increasing the rate of flow from the pumping system to a multiple of the predetermined rate of flow starting with a first of said two boundary conditions; and

    reducing said rate of flow from the pumping system to said predetermined rate of flow when said characteristic is between the first boundary condition and the last boundary condition at a point which is a reciprocal of said multiple of the distance between the first and the last boundary condition.

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