Luminiscence measurement arrangement

• US 4,772,453 A
• Filed: 03/01/1985
• Issued: 09/20/1988
• Est. Priority Date: 03/01/1985
• Status: Expired due to Term

• Current Assignee: Dynex Technologies, Inc.
• Original Assignee: Wayne F. Lisenbee
• Inventors: Lisenbee, Wayne F.
• Primary Examiner(s): NOT, DEFINED
• Application Number:
  US06/707,496
• Time in Patent Office: 1,299 Days
• Field of Search: 422/52, 422/58, 422/63, 422/104, 422/102, 422/65, 422/73, 436/47, 436/165, 436/172, 436/809, 269/63, 269/71, 435/291, 435/808, 414/749, 414/750
• US Class Current: 422/52
• CPC Class Codes: G01N 21/76 Chemiluminescence; Bioluminescence
• CPC Class Codes: Y10S 436/809 Multifield plates or multicontainer arrays

Abstract

A high speed, luminescence detection arrangement for sequentially measuring the luminescence emitted from a plurality of luminescence sources under test. A tray holder contains a plurality of cup-like sample wells, from which the luminescent radiation is emitted, and each of the cup-like sample wells is sequentially positioned in a predetermined location to allow the detection and measurement of the intensity of the luminescence emitted therefrom. Display means are provided to provide a detectible display, having a magnitude proportional to the intensity of the luminescence detected. Fluid dispensing means are operatively interconnected to allow injection of one or more fluids into each of the cup-like sample wells at a predetermined time before measurement of the luminescence emitted therefrom.

Claims

1. A luminescence measurement arrangement comprising, in combination:

a case means, having first walls comprising a top wall portion, opposed side wall portions, a front wall portion, and a back wall portion, said first walls defining a base chamber, and second walls defining an upper chamber, said upper chamber separated from said base chamber by said top wall portion of said first walls;

said top wall portion of said first walls having an upper surface in said upper chamber, and a lower surface in said base chamber, and said top wall portion having edge walls defining an aperture therethrough, providing communication between said base chamber and said upper chamber;

said front wall portion of said first walls having edges defining a tray holder receiving aperture therethrough, providing communication between said base chamber and regions external thereof;

tray holder means movably mounted in said base chamber and having a load position extending through said tray receiving aperture in regions external said base chamber and movable to a plurality of internal positions in said base chamber, said tray holder means for receiving a tray having a plurality of cup-like sample wells in a predetermined spaced array;

motion producing means mounted on said top wall portion and operatively connected to said tray holder means for selectively moving said tray holder means between said load position to said plurality of internal positions, whereby a plurality of cup-like sample wells is positioned in a predetermined sequence in an aligned condition with said aperture in said top wall portion;

luminescence detection means mounted on said top wall portion in said upper chamber for detecting the luminescence emitted from one of a plurality of cup-like sample wells in an aligned condition with said aperture in said top wall portion for said tray holder means in each of said plurality of internal positions thereof, and generating an output signal having a magnitude proportional to the intensity of the detected luminescence;

display means for receiving said output signal from said luminescence detection means and providing a detectible display proportional to said output signal in regions external said case means.

2. The arrangement defined in claim 1, wherein:

said base chamber is substantially free of light emanating from regions external thereof, for the condition of said tray holder means in said plurality of internal positions thereof.

3. The arrangement defined in claim 2, wherein:

said motion producing means further comprises drive means for moving said tray holder means in mutually perpendicular directions in said base chamber.

4. The arrangement defined in claim 3, wherein:

said drive means further comprises a first drive means for moving said tray holder in first directions of said mutually perpendicular directions, and a second drive means for moving said tray holder means in second directions of said mutually perpendicular directions.

5. The arrangement defined in claim 4, wherein:

at least one of said first drive means and said second drive means comprises a Geneva drive means.

6. The arrangement defined in claim 4, wherein:

said first drive means and said second drive means further comprise load position indicating means for detecting said tray holder means in said load position thereof, and generating first position signals in response thereto.

7. The arrangement defined in claim 6, wherein:

at least one of said first and second drive means further comprises internal position detection means for detecting a preselected number of said plurality of internal positions of said tray holder means and generating second position signals in response thereto.

8. The arrangement defined in claim 4, wherein:

said first drive means further comprises;

a first Geneva drive means mounted on said upper surface of said top wall portion in said upper chamber, and having a rotatable first drive shaft means extending through said top wall portion into said base chamber;

a first pulley and a second pulley rotatably mounted on said lower surface of said top wall portion in said base chamber, and in spaced apart relationship, for rotation about a first axis and second axis, respectively, said first pulley mounted on said first drive shaft for rotation therewith;

first belt means around said first pulley and said second pulley, and movable therearound for the condition of rotation of said first pulley;

first guide rod means mounted on, and spaced from, said lower surface of said top wall portion in said base chamber, and aligned substantially parallel to the plane containing said first axis and said second axis;

first slide means, slidably mounted on said first guide rod means and coupled to said first belt means for movement therewith between said first pulley and said second pulley, in said first directions of said mutually perpendicular directions; and

said tray holder means operatively connected to said first slide means for movement therewith.

9. The arrangement defined in claim 8, wherein:

said second drive means further comprises;

a second rotatable drive shaft, extending through said top wall portion from said upper chamber to said base chamber;

a third pulley and a fourth pulley, rotatably mounted on said lower surface of said top wall portion in said beam chamber for rotation about a third axis and a fourth axis, respectively, and said third axis and said fourth axis in a plane substantially perpendicular to the plane containing said first axis and said second axis, and said third pulley mounted on said second drive shaft for rotation therewith;

second belt means around said third pulley and said fourth pulley for motion therearound for the condition of rotation of said third pulley;

second guide rod means and third guide rod means mounted on and spaced from said lower surface of said top wall portion in said base chamber in parallel, aligned, spaced apart relationship and parallel to the plane containing said third axis and said fourth axis;

second slide means, slidably mounted on said second guide rod means and coupled to said second belt means for movement therewith, between said third pulley and said fourth pulley, said second directions of said mutually perpendicular directions; and

said tray holder means operatively connected to said second slide means for movement therewith.

10. The arrangement defined in claim 9, wherein:

said motion producing means further comprises;

third slide means, slidingly mounted on said third guide rod means for sliding movement therealong;

fourth guide rod means coupled to said second slide means and said third slide means, and extending therebetween, and said fourth guide rod means substantially parallel to said first guide rod means for movement with said second and third slide means;

fourth slide means, coupled to said tray holder means and slidingly mounted on said fourth guide rod means for movement therewith;

fifth guide rod means, coupled to said first slide means and extending therefrom in a direction substantially parallel to said third guide rod means and said fourth guide rod means; and

fifth slide means, coupled to said tray holder means and slidably mounted on said fifth guide rod means for movement therealong.

11. The arrangement defined in claim 10, wherein

said second drive means further comprises:

a stepping motor means mounted on said upper surface of said top wall portion in said upper chamber for rotating said second drive shaft.

12. The arrangement defined in claim 10, wherein:

said second drive means further comprises;

a second Geneva drive means, mounted on said upper surface of said top wall portion for rotating said second drive shaft.

13. The arrangement defined in claim 10, wherein:

said fourth slide measns, comprises two spaced apart slide bearing means; and

said fifth slide means, comprises two spaced apart slide bearing means.

14. The arrangement defined in claim 10, wherein:

said motion producing means further comprises drive control means for selectively energizing said first drive means and said second drive means to selectively rotate said first drive shaft and said second drive shaft, whereby said tray holder means is selectively moved in said mutually perpendicular direction.

15. The arrangement defined in claim 4, wherein:

said luminescence detection means further comprises a photo-multiplier tube.

16. The arrangement defined in claim 15, wherein:

said luminescence detection means further comprises;

plug means having walls defining a lens aperture therethrough mounted in said aperture in said top wall portion and having a lens means therein for transmitting said luminescence from the cup-like sample well in aligned relationship to said lens aperture into said upper chamber;

prism means for receiving said luminescence from said lens means and directing said luminescence to said photo-multiplier tube; and

light shield means for shielding said photo-multiplier tube from ambient light external said light shield means.

17. The arrangement defined in claim 16, wherein:

said lens aperture in said plug means has a smaller cross section area than each of the cross section areas of the open end of the cup-like sample wells; and

one of said plurality of said cup-like sample wells aligned with said lens aperture at each of said internal positions of said tray holder means; and

said tray holder means further comprises;

mask means mounted thereon and having a wall defining a plurality of mask apertures therethrough, said plurality of mask apertures corresponding to the number of the plurality of cup-like sample wells in the tray, and one of said apertures aligned with each of the cup-like sample wells and having a cross sectional diameter substantially corresponding to the cross sectional area of the cup-like sample wells.

18. The arrangement defined in claim 17, wherein:

said tray holder means further comprises;

tray support means movable from a load position to a detection position for positioning a tray of cup-like sample wells closely adjacent said mask means for tray holder means in the plurality of internal positions thereof.

19. The arrangement defined in claim 17, and further comprising:

calibration light source means, mounted in said base chamber for selectively emitting a predetermined intensity of light through said lens aperture.

20. The arrangement defined in claim 17, wherein:

said tray holder means further comprises;

alignment means for aligning the tray of cup-like sample wells in a predetermined position therein, for alignment with said mask means.

21. The arrangement defined in claim 8, wherein:

said motion producing means further comprises;

sequencing means for receiving said first position signals and said second position signals and selectively controlling said first drive means and said second drive means in response thereto.

22. The arrangement defined in claim 1, wherein:

said display means further comprises;

printer means for sequentially providing a digitalized printed display, corresponding to the intensity of luminescence detected from each cup-like sample well.

23. The arrangement defined in claim 1, and further comprising:

fluid dispensing means, operatively connected to said case means for selectively dispensing predetermined fluids in a predetermined timed sequence into each cup-like sample well.

24. The arrangement defined in claim 23, wherein:

said fluid dispensing means further comprises;

control means for selectively and sequentially dispensing more than one fluid into each cup-like sample well.

25. The arrangement defined in claim 23, wherein:

said fluid dispensing means dispenses fluid into a cup-like sample well before such cup-like sample well is in an aligned condition with said aperture in said top wall portion of said first walls.

26. A luminescence measurement arranged comprising, in combination:

a case means, having walls defining a cavity;

tray holder means movably mounted to said case means and having a load position extending external said case means and movable to a plurality of internal positions in said case means, said tray holder means for receiving a tray having a plurality of cup-like sample wells in a predetermined spaced array;

motion producing means mounted on said case means and operatively connected to said tray holder means for selectively moving said tray holder means between said load position to said plurality of internal positions, whereby a plurality of cup-like sample wells are positioned in a predetermined sequence in predetermined positions in said case means;

luminescence detection means mounted on said case means in luminescence receiving relationship to the plurality of cup-like sample wells for detecting the luminescence emitted from one of a plurality of cup-like sample wells in each of said plurality of internal positions thereof, and generating an output signal having a magnitude proportional to the intensity of the detected luminescence;

display means for receiving said output signal from said luminescence detection means and providing a detectible display proportional to said output signal in regions external said case means.