Chemically cleavable nucleotides
First Claim
1. A compound having the structure:
- ##STR12## wherein B is selected from purine, 7-deazapurine and pyrimidine provided that when B is purine or 7-deazapurine, it is attached at the N9 -position of purine or deazapurine, and when B is pyrimidine, it is attached at the N1 -position;
wherein A represents biotin or iminobiotin wherein ...D... is selected from the formulae;
##STR13## D is a chemically cleavable disulfide bond and ...D... joins B and A, provided that if B is purine, the linkage is attached to the 8-position of the purine, if B is 7-deazapurine, the linkage is attached to the 7-position of the deazapurine, and if B is pyrimidine, the linkage is attached to the 5-position of the pyrimidine; and
wherein each of x, y and z represents ##STR14##
1 Assignment
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Accused Products
Abstract
Novel biotinylated nucleotides have a chemically cleavable linker arm between a biotin and an organic basic group. They are useful in a method of isolating target macromolecules from crude physiological mixtures. The biotinylated nucleotides are bound via their organic basic groups to macromolecules having an affinity for the target macromolecules and brought into contact with the target macromolecules to form a biotinylated nucleotide-affinity macromolecule - target macromolecule complex. The complex thus obtained is brought into contact with immobilized avidin whereupon the biotin moeity binds to the avidin. The complex and avidin are washed to remove undesired substances and then the chemically cleavable bond in the nucleotide is cleaved to obtain the affinity-macromolecule - target macromolecule complex from which the target macromolecule can be obtained.
187 Citations
7 Claims
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1. A compound having the structure:
- ##STR12## wherein B is selected from purine, 7-deazapurine and pyrimidine provided that when B is purine or 7-deazapurine, it is attached at the N9 -position of purine or deazapurine, and when B is pyrimidine, it is attached at the N1 -position;
wherein A represents biotin or iminobiotin wherein ...D... is selected from the formulae;
##STR13## D is a chemically cleavable disulfide bond and ...D... joins B and A, provided that if B is purine, the linkage is attached to the 8-position of the purine, if B is 7-deazapurine, the linkage is attached to the 7-position of the deazapurine, and if B is pyrimidine, the linkage is attached to the 5-position of the pyrimidine; and
wherein each of x, y and z represents ##STR14## - View Dependent Claims (2, 3, 4)
- ##STR12## wherein B is selected from purine, 7-deazapurine and pyrimidine provided that when B is purine or 7-deazapurine, it is attached at the N9 -position of purine or deazapurine, and when B is pyrimidine, it is attached at the N1 -position;
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5. A compound of the formula:
- ##STR15## in which D is a chemically cleavable disulfide bond and W is a single bond or ##STR16## and n is 2 to 6.
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6. A compound of the formula:
- ##STR17##
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7. A compound of the formula:
- ##STR18##
Specification