Pharmaceutical administration systems containing a mixture of immunomodulators

• US 4,774,085 A
• Filed: 07/09/1985
• Issued: 09/27/1988
• Est. Priority Date: 07/09/1985
• Status: Expired due to Term

• Current Assignee: Board of Regents of the University of Texas System (University of Texas System)
• Original Assignee: Board of Regents of the University of Texas System (University of Texas System)
• Inventors: Fidler, Isaiah J.
• Primary Examiner(s): HAZEL, BLONDEL
• Application Number:
  US06/753,192
• Time in Patent Office: 1,176 Days
• Field of Search: 424/85
• US Class Current: 424/85.5
• CPC Class Codes: A61K 2300/00 Mixtures or combinations of active ingredients, wherein at least one active ingredient is fully defined in groups A61K31/00 - A61K41/00
• CPC Class Codes: A61K 38/05 Dipeptides
• CPC Class Codes: A61K 38/21 Interferons [IFN]
• CPC Class Codes: A61K 38/217 IFN-gamma
• CPC Class Codes: A61K 9/127 Liposomes
• CPC Class Codes: Y10S 930/142 Interferon

Abstract

The present invention relates to pharmaceutical administration systems containing phosphatidylserine and phosphatidylcholine or phosphatidylethanolamine derivatives in the form of liposomes which encapsulate water soluble muramyldipeptide derivatives in combination with gamma-interferon. The liposomes are prepared by conventional dispersion methods. The pharmaceutical administration systems when applied in the form of liposomes are especially useful in the cancer chemotherapy for combating metastatic tumor cells.

Claims

1. A pharmaceutical administration system consisting of

(a) a water soluble muramylpeptide,

(b) gamma-interferon,

(c) a phospholipid of the formula ##STR7## wherein n is one, two or three, R₁ and R₂ independently of each other represent alkyl, alkenyl or acyl each having 10-20 carbon atoms, and Y.sup.⊕

is the cation of a pharmaceutically acceptable base,

(d) a phospholipid of the formula ##STR8## wherein n is two, three or four, R₁ and R₂ are defined as above and R₃, R₄ and R₅ represent hydrogen or C₁ -C₄ -alkyl, and, optionally, a pharmaceutically acceptable carrier solution buffered to pH 7.0-7.8, and, optionally a pharmaceutically acceptable carrier in solid form.

2. A pharmaceutical administration system according to claim 1 consisting of

(a) a muramylpeptide of the formula ##STR9## wherein X represents the groups --C(═

O)-- or --C(═

O)--O--;

R₁ represents C₁ -C₄ -alkyl;

R₂, R₃ and R₄ indpendently of each other represent or C₁ -C₄ -alkyl;

R₅ and R₆ independently of each other represent carbamoyl, carboxy or esterified carboxy;

when R₃ is other than hydrogen, the configuration about the carbon atom designated (I*) is D;

when R₄ is other than hydrogen the configuration about the carbon atom designated (2*) is L; and

when R₅ is other than hydrogen the configuration about the carbon atom designated (3*) is D;

or a pharmaceutically acceptable salt thereof,

(b) purified, natural or recombinant human gamma interferon,

(c) a phospholipid of the formula I wherein n, R₁, R₂ and Y.sup.⊕

are defined as above,

(d) a phospholipid of the formula II wherein n and R₁ to R₅ are defined as above, and, optionally, a pharmaceutically acceptable carrier solution buffered from pH 7.2 to 7.4.

3. A pharmaceutical administration system according to claim 1 consisting of

(a) sodium-N-acetyl-D-muramyl-L-alanyl-D-isoglutamine or sodium-N-acetyldesmethylmuramyl-L-alanyl-D-isoglutamine,

(b) purified, natural or recombinant human gamma interferon,

(c) a phospholipid of the formula I wherein n is one, R₁ and R₂ are acyl each having 10 to 20 carbon atoms and Y.sup.⊕

is the sodium ion,

(d) a phospholipid of the formula II wherein n is two, R₁ and R₂ are acyl each having 10 to 20 carbon atoms, and R₃ to R₅ represent hydrogen or methyl, and, optionally, a pharmaceutically acceptable carrier solution buffered from pH 7.2 to 7.4.

4. A pharmaceutical administration system according to claim 1 consisting of

(a) sodium-N-acetyl-D-muramyl-L-alanyl-D-isoglutamine or sodium-N-acetyldesmethylmuramyl-L-alanyl-D-isoglutamine,

(b) purified, natural or recombinant human gamma interferon,

(c) a synthetic phospholipid of the formula I wherein n is one, R₁ and R₂ are identical C₁₀ -C₂₀ -alkenoyl groups and Y.sup.⊕

is the sodium ion,

(d) a synthetic phospholipid of the formula II, wherein R₁ and R₂ are identical C₁₀ -C₂₀ -alkanoyl groups and R₃ to R₅ represent methyl, and optionally, a pharmaceutically acceptable carrioer solution buffered from pH 7.2 to 7.4.

5. A pharmaceutical administration system according to claim 1 consisting of

(a) sodium-N-acetyl-D-muramyl-L-alanyl-D-isoglutamine or sodium-N-acetyldesmethylmuramyl-L-alanyl-D-isoglutamine,

(b) purified, natural or recombinant, human gamma-interferon,

(c) synthetic, essentially pure sodium-1,2-di-(9-cis-octadecenoyl)-3-sn-phosphatidyl-(S)-serine (I),

(d) synthetic, essentially pure 1-n-hexadecanoyl-2-(9-cis-octadecenoyl)-3-sn-phosphatidylcholine (II), and, optionally, a pharmaceutically acceptable carrier solution.

6. A pharmaceutical administration system according to claim 1 consisting of

(a) 50 to 200 microgramm of sodium-N-acetyl-D-muramyl-L-alanyl-D-isoglutamine or sodium-N-acetyldesmethylmuramyl-L-alanyl-D-isoglutamine,

(b) about 1,000 to 100,000 units of recombinant gamma-interferon having the amino acid sequences:

##STR10## and approximately 200 mg to 1,000 mg of (c) synthetic, essentially pure sodium-1,2-di-(9-cis-octadecenoyl)-3-sn-phosphatidyl-(S)-serine(I) and

(d) synthetic, essentially pure 1-n-hexadecanoyl-2-(9-cis-octadecenoyl)-3-sn-phosphatidylcholine (II), wherein the molar ratio of the phospholipid component (c) to the phospholipid component (d) is about 30 to 70 mole percent, and, optionally, a pharmaceutically acceptable carrier solution.

7. A method of stimulating the immune system in humans or animals which comprises administering to a human or animal in need of such administration an effective amount of a pharmaceutical administration system as claimed in claim 1.

8. A method of activating macrophages or monocytes in humans which comprises administering to a human in need of such administration an effective amount of a pharmaceutical administration system as claimed in claim 1.