Chemically reactive dipyrrometheneboron difluoride dyes
First Claim
1. Fluorescent dyes with the common structure, ##STR5## wherein R1, R2, R3, R4, R5, and R6 are chosen among the substituents hydrogen, halogen, alkyl, cycloalkyl, aryl, arylalkyl, acyl, and sulfo alone or in combination where at least one of the groups is further modified to yield a chemically reactive group capable of forming a chemical bond with a ligand.
2 Assignments
0 Petitions
Accused Products
Abstract
Novel fluorescent dyes based on the dipyrrometheneboron difluoride structure are provided. The new reagents contain functional groups capable of forming a stable fluorescent product with functional groups typically found in biomolecules or polymers including amines, phenols, thiols, acids, aldehydes and ketones. Reactive groups in the dipyrrometheneboron difluoride dyes include activated esters, isocyanates, amines, hydrazines, sulfonyl halides, acids, aldehydes, alcohols and haloacetamides. The products are detected by their absorbance or fluorescence properties. The spectral properties of the fluorescent dyes are sufficiently similar in wavelengths and intensity to fluorescein as to permit use of the same euqipment. The dyes, however, have narrower spectral bandwidths than fluorescein, do not show appreciable sensitivity to pH, have higher solubility in non-polar solvents and have improved photostability.
337 Citations
13 Claims
- 1. Fluorescent dyes with the common structure, ##STR5## wherein R1, R2, R3, R4, R5, and R6 are chosen among the substituents hydrogen, halogen, alkyl, cycloalkyl, aryl, arylalkyl, acyl, and sulfo alone or in combination where at least one of the groups is further modified to yield a chemically reactive group capable of forming a chemical bond with a ligand.
Specification
-
- Resources
-
Current AssigneeMolecular Probes Incorporated (Thermo Fisher Scientific Incorporated)
-
Original AssigneeMolecular Probes Incorporated (Thermo Fisher Scientific Incorporated)
-
InventorsHaugland, Richard P., Kang, Hee C.
-
Primary Examiner(s)Shaver, Paul F.
-
Application NumberUS07/083,458Time in Patent Office414 DaysField of Search564/11, 564/10, 260/349, 260/549, 548/545, 548/405US Class Current548/405CPC Class CodesC07F 5/022 without C-boron linkagesG01N 33/582 with fluorescent label
