Process for producing L-arginine

• US 4,775,623 A
• Filed: 08/31/1984
• Issued: 10/04/1988
• Est. Priority Date: 09/24/1983
• Status: Expired due to Term

• Current Assignee: Kyowa Hakko Kogyo Company Limited (Kirin Holdings Company Limited)
• Original Assignee: Kyowa Hakko Kogyo Company Limited (Kirin Holdings Company Limited)
• Inventors: Oka, Tetsuo, Yokoi, Haruhiko, Katsumata, Ryoichi
• Primary Examiner(s): Wiseman, Thomas G.
• Assistant Examiner(s): NOT, DEFINED
• Application Number:
  US06/646,512
• Time in Patent Office: 1,495 Days
• Field of Search: 435/114, 435/172.3, 435/253, 435/317, 435/840, 435/843, 935/29, 935/60, 935/72
• US Class Current: 435/114
• CPC Class Codes: C12P 13/04 Alpha- or beta- amino acids other amino acids C12P13/005
• CPC Class Codes: C12P 13/06 Alanine; Leucine; Isoleucine; Serine; Homoserine
• CPC Class Codes: C12P 13/10 Citrulline; Arginine; Ornithine
• CPC Class Codes: C12P 13/22 Tryptophan; Tyrosine; Phenylalanine; 3,4-Dihydroxyphenylalanine
• CPC Class Codes: C12P 13/24 Proline; Hydroxyproline; Histidine
• CPC Class Codes: Y10S 435/84 Brevibacterium
• CPC Class Codes: Y10S 435/843 Corynebacterium

Abstract

Disclosed is a process for producing L-arginine by transforming a host microorganism belonging to the genus Corynebacterium or Brevibacterium with a recombinant DNA of a DNA fragment containing a gene involved in the biosynthesis of L-arginine and a vector DNA, culturing the transformant in a nutrient medium, accumulating L-arginine in the culture medium and recovering L-arginine therefrom.

Claims

1. A process for producing L-arginine which comprises the steps of transforming a host microorganism belonging to the genus Corynebacterium or Brevibacterium with a vector containing a DNA fragment comprising an Escherichia coli gene coding for N-acetylglutamokinase, culturing the transformant in a nutrient medium, accumulating L-arginine in the culture medium an recovering L-arginine therefrom.

2. A process for producing L-arginine which comprises the steps of culturing a Corynebacterium or Brevibacterium host microorganism harboring a vector containing a DNA fragment comprising an Escherichia coli gene coding for N-acetylglutamokinase in a nutrient medium, accumulating L-arginine in the culture medium and recovering L-arginine therefrom.

3. A microorganism belonging to the genus Corynebacterium or Brevibacterium which harbors a vector containing a DNA fragment comprising an Escherichia coli gene coding for N-acetyl-glutamokinase.

4. Corynebacterium glutamicum K-46 (FERM BP-356).

5. Corynebacterium herculis K-47 (FERM BP-367).

6. Brevibacterium flavum K-48 (FERM BP-357).