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Method for measuring antigens or antibodies in biological fluids using ligand labeled antigens or ligand labeled antibodies

  • US 4,778,751 A
  • Filed: 05/12/1986
  • Issued: 10/18/1988
  • Est. Priority Date: 05/12/1986
  • Status: Expired due to Term
First Claim
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1. A method for measuring the level of an antigen (Ag1), antibody (Ab1) or hapten (H) in a liquid sample, which sequentially comprises:

  • (a) forming in a liquid phase reaction a soluble complex wherein said antigen (Ag1), antibody (Ab1) or hapten (H) is linked through, respectively, a specific antibody (Ab), antigen (Ag) or antihapten (Anti-H), to a matrix which is soluble in the liquid phase and carries a ligand (X), said matrix capable of being chemically attached to more than one specific antibody (Ab), antigen (Ag) or antihapten (Anti-H);

    (b) forming an insolubilized complex comprising a solid support linked to the ligand (X) of said soluble complex through an anti-ligand (Y), the insolubilized complex carrying a label (Z) linked to the antigen (Ag1) through an anti-antigen (Anti-Ag1), to the antibody (Ab1) through an anti-antibody (Anti-Ab1) or to the hapten (H);

    (c) washing the insolubilized complex; and

    (d) observing the washed insolubilized complex for the presence of the label (Z) wherein the presence of the label (Z) is an indication of the level of said antigen (Ag1), antibody (Ab1) or hapten (H) in said sample.

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