L-glutamine sensor

• US 4,780,191 A
• Filed: 06/26/1987
• Issued: 10/25/1988
• Est. Priority Date: 06/26/1987
• Status: Expired due to Fees

• Current Assignee: Institute of Technology (Select Education Group LLC)
• Original Assignee: Massachusetts Institute of Technology
• Inventors: Romette, Jean L., Cooney, Charles L.
• Primary Examiner(s): Kaplan, G. L.
• Application Number:
  US07/067,641
• Time in Patent Office: 487 Days
• Field of Search: 204/1 E, 204/403, 204/415, 435/291, 435/4, 435/16, 435/25, 435/817
• US Class Current: 204/403.1
• CPC Class Codes: C12Q 1/005 involving specific analytes or enzymes including groups of enzymes, e.g. oxydases; C12Q1/004 takes precedence
• CPC Class Codes: Y10S 435/817 Enzyme or microbe electrode

Abstract

Apparatus for measuring L-glutamine in a liquid sample includes a membrane on which are immobilized the enzymes glutaminase and glutamate oxidase, whereby any said L-glutamine in the sample is acted upon by the glutaminase to form glutamic acid, the glutamic acid being acted upon by the glutamate oxidase to form an enzymatic reaction product, the membrane being operatively associated with a sensor capable of sensing the enzymatic reaction product or a compound or element consumed or liberated in the formation or degradation thereof.

Claims

1. Apparatus for measuring L-glutamine in a liquid sample, comprising a membrane on which are immobilized the enzymes glutaminase and glutamate oxidase, whereby any said L-glutamine in said sample is acted upon by said glutaminase to form glutamic acid,. said glutamic acid being acted upon by said glutamate oxidase to form an enzymatic reaction product, said membrane being operatively associated with a sensor capable of sensing said enzymatic reaction product or a compound or element consumed or liberated in the formation or degradation thereof.

2. The apparatus of claim 1 wherein said glutaminase and said glutamine oxidase are immobilized in an insolubilized protein containing matrix.

3. The apparatus of claim 1 wherein said sensor measures dissolved oxygen and said membrane is oxygen permeable.

4. The sensor of claim 2 wherein said insolubilized protein matrix comprises gelatin.