Apparatus for supplying a medium to a reaction chamber

• US 4,783,413 A
• Filed: 05/14/1987
• Issued: 11/08/1988
• Est. Priority Date: 07/30/1986
• Status: Expired due to Fees

• Current Assignee: Oerlikon Contraves AG
• Original Assignee: Contraves AG
• Inventors: Suter, Robert N.
• Primary Examiner(s): Jones, Larry
• Application Number:
  US07/049,436
• Time in Patent Office: 544 Days
• Field of Search: 435/313, 435/284, 435/285, 435/286, 435/289, 435/288, 604/892
• US Class Current: 435/286.5
• CPC Class Codes: C12M 29/00 Means for introduction, extraction or recirculation of materials, e.g. pumps pumps per se F04B
• CPC Class Codes: C12M 41/00 Means for regulation, monitoring, measurement or control, e.g. flow regulation controlling or regulating chemical, physical or physicochemical processes B01J19/0006; heating or cooling apparatus for laboratory use B01L7/00; electro optical investigation of individual particles, flow cytometers G01N15/14; automatic analysis G01N35/00; controlling or regulating in general G06N
• CPC Class Codes: C12M 41/40 of pressure

Abstract

An osmotic pump for supplying a fluid medium to a reaction chamber defining a first chamber, for example, for supplying a fluid nutrient to a cell culture located in the reaction chamber, is connected to the reaction chamber by a plurality of channels forming a channel system. The osmotic pump provides a continual supply of fluid medium even under zero gravity conditions. The removal and return of expended reaction medium takes place by means of a channel into a second chamber. This second chamber encloses an operating medium for the osmotic pump such that the chambers form a closed system with the corresponding channels.

Claims

1. An apparatus for supplying a fluid medium to a reaction chamber, comprising:

at least two chambers defining a first chamber and a second chamber;

said first chamber comprising a reaction chamber for receiving a reaction medium;

an osmotic pump containing the reaction medium and for supplying the reaction medium to said reaction chamber;

at least one other chamber of said at least two chambers defining said second chamber located adjacent to said osmotic pump and containing an operating medium for operating said osmotic pump;

a channel system containing a plurality of channels rheologically interconnecting said at least two chambers for supplying the reaction medium from said osmotic pump to said reaction chamber and removing expended reaction medium from said reaction chamber;

said second chamber, said osmotic pump, said reaction chamber and at least a portion of said channels of said channel system forming a closed system;

a control sleeve having a longitudinal axis and being mounted for rotation about its longitudinal axis;

said control sleeve being arranged in flow communication with said osmotic pump;

said plurality of channels including a channel leading from said control sleeve to said reaction chamber and a bypass channel bypassing said reaction chamber and leading from said control sleeve to said second chamber; and

said control sleeve, depending upon its rotational position, selectively providing flow communication between said control sleeve and either one of (i) said channel leading to said reaction chamber or (ii) said bypass channel.

2. The apparatus as defined in claim 1, further including:

a housing provided for said osmotic pump and said reaction chamber;

said control sleeve having a base portion and being rotatably mounted in said housing for rotation about said longitudinal axis;

said osmotic pump being mounted in said control sleeve;

said control sleeve comprising at least one opening for operatively connecting said second chamber containing said operating medium to said osmotic pump;

said plurality of channels including an angled discharge channel means located in said base portion of said control sleeve;

said base portion containing two discharge ports;

one of said two discharge ports communicating with said channel leading to said reaction chamber and an other one of said two discharge ports communicating with said bypass channel; and

said angled discharge channel means located in said base portion of said control sleeve, depending upon the rotational position of said control sleeve, selectively providing flow communication between said angled discharge channel means and a desired one of said channel leading to said reaction chamber and said bypass channel.

3. The apparatus as defined in claim 1, wherein:

said channel system comprises an elongated connecting channel containing said operating medium for operating said osmotic pump and being connected with said reaction chamber and said second chamber; and

said elongated connecting channel containing means for limiting mixture of said expended reaction medium issuing from said reaction chamber with said operating medium contained within said second chamber.

4. The apparatus as defined in claim 3, wherein:

said elongated connecting channel comprises an upper channel section; and

said mixture limiting means constituting a freely floating piston located in said upper channel section for separating said operating medium contained within said second chamber from the expended reaction medium issuing from the reaction chamber.

5. The apparatus as defined in claim 3, further including:

means for rendering accessible said reaction chamber for withdrawing a sample from said reaction chamber.

6. The apparatus as defined in claim 3, further including:

a closure plug closing an end of said elongated connecting channel and which end is remote from said second chamber; and

said elongated connecting channel being accessible through said closure plug for withdrawing a sample of the expended reaction medium contained in said elongated connecting channel.

7. The apparatus as defined in claim 3, wherein:

said reaction chamber comprises an exit means leading to said elongated connecting channel; and

a filter element positioned upstream of said exit means of said reaction chamber.

8. The apparatus as defined in claim 1, wherein:

said reaction chamber is provided with at least one glass disc for monitoring the contents of the reaction chamber.