Fluorescence imaging system

• US 4,786,813 A
• Filed: 06/23/1986
• Issued: 11/22/1988
• Est. Priority Date: 10/22/1984
• Status: Expired due to Term

• Current Assignee: Spectraphos AB OLE Romers VAG
• Original Assignee: Hightech Network AB
• Inventors: Svanberg, Sune, Montan, Sune
• Primary Examiner(s): Howell, Janice A.
• Assistant Examiner(s): Rauchholz, William F.
• Application Number:
  US06/882,975
• Time in Patent Office: 883 Days
• Field of Search: 250/461.2, 250/461.1, 250/459.1, 250/458.1, 250/578, 350/613, 350/616
• US Class Current: 250/461.1
• CPC Class Codes: G01J 2003/1239 and separate detectors
• CPC Class Codes: G01J 3/02 Details
• CPC Class Codes: G01J 3/021 using plane or convex mirrors, parallel phase plates, or particular reflectors
• CPC Class Codes: G01J 3/0237 Adjustable, e.g. focussing
• CPC Class Codes: G01J 3/36 Investigating two or more bands of a spectrum by separate detectors
• CPC Class Codes: G01N 2021/6421 Measuring at two or more wavelengths
• CPC Class Codes: G01N 21/6456 Spatial resolved fluorescence measurements; Imaging

Abstract

A fluorescence imaging system. A light source irradiates an object to be viewed. The irradiated object is imaged through a beam-splitting system to form individual displaced images of the object. The displaced images are further filtered in a filter having a frequency passband different from the other filters. The filtered images are imaged on a detector. The same image point of each image is detected and converted into an electrical signal. A circuit means is provided to receive the signals of each common image point, and weight the same. The weighted signal represents an image point pixel. The weighted pixels are displayed as an image of the object having improved contrast.

Claims

1. A fluorescence imaging system comprising:

a light source for irradiating an object to be viewed;

a beam-splitting system for forming individual displaced images of said irradiated object;

a plurality of wavelength filters disposed in the path of said individual images, said filter selected to have different passband frequencies, such that said images are filtered differently;

a detector for receiving said individual displaced images which pass through said filters, said detector providing a plurality of signals representing the same image point of each of said displaced images; and

,

circuit means connected to receive said signals representing the same image point for each of said displaced images, said circuit means generating a weighted signal value of said individual signals, such that a single image signal is produced for each image point of an image of said object.

2. A fluorescence imaging system according to claim 1, wherein the beam-splitting system comprises a mirror, which is incorporated in the optical system and divided into several parts, said parts being mutually angled at a position to image the object on different parts of the plane of said detector.

3. A fluorescence imaging system according to claim 2, wherein the divided mirror is a concave mirror, with each part thereof also being concave.

4. A fluorescence imaging system according to claim 1, wherein the filters are interference filters.

5. A fluorescence imaging system according to claim 1, wherein the weighted signal value is dimensionless and unrelated to absolute values of signal intensity.

6. A fluorescence imaging system according to claim 1, wherein an optical imaging system which images the object in an object plane, is arranged, in the light path, between the beam-splitting system and said object.

7. A fluorescence imaging system according to claim 6, wherein the light source includes a second beam-splitting system, to irradiate the object via the optical imaging system.

8. A fluorescence imaging system according to claim 1, characterized in that the light source is pulsatable laser;

and in that means are provided for activating the detector means at each laser pulse.