Method and apparatus for obtaining and delivering a predetermined quantity of plasma from a blood sample for analysis purposes

• US 4,788,154 A
• Filed: 12/22/1986
• Issued: 11/29/1988
• Est. Priority Date: 12/20/1985
• Status: Expired due to Fees

• Current Assignee: Jean Guigan
• Original Assignee: Jean Guigan
• Inventors: Guigan, Jean
• Primary Examiner(s): Castel, Benoit
• Assistant Examiner(s): HILL JR, ROBERT J
• Application Number:
  US06/944,406
• Time in Patent Office: 708 Days
• Field of Search: 356/246, 422/61, 422/72, 422/64, 422/102, 436/45, 436/180, 494/16, 210/514, 210/518, 210/787
• US Class Current: 436/180
• CPC Class Codes: B01L 2200/0621 Control of the sequence of chambers filled or emptied
• CPC Class Codes: B01L 2300/0809 rectangular shaped
• CPC Class Codes: B01L 2300/0864 comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
• CPC Class Codes: B01L 2300/0867 Multiple inlets and one sample wells, e.g. mixing, dilution
• CPC Class Codes: B01L 2300/087 Multiple sequential chambers
• CPC Class Codes: B01L 2400/0409 centrifugal forces
• CPC Class Codes: B01L 2400/0683 mechanically breaking a wall or membrane within a channel or chamber
• CPC Class Codes: B01L 3/502753 characterised by bulk separation arrangements on lab-on-a-chip devices, e.g. for filtration or centrifugation separation in general B01D; microapparatus for analysis using electrophoresis G01N27/44791; sample preparation G01N1/28
• CPC Class Codes: G01N 21/07 Centrifugal type cuvettes G01N21/09 takes precedence
• CPC Class Codes: G01N 33/48 Biological material, e.g. blood, urine G01N33/02, G01N33/26, G01N33/44, G01N33/46 take precedence; Haemocytometers counting blood corpuscules distributed over a surface by scanning the surface G06M11/02
• CPC Class Codes: Y10T 436/111666 Utilizing a centrifuge or compartmented rotor
• CPC Class Codes: Y10T 436/2575 Volumetric liquid transfer

Abstract

A method and a device for obtaining plasma from a few microliters of blood for analysis purposes. The method makes use a transfer strip (10) and a turntable for performing centrifuging operations.

Claims

1. A method of delivering at least one predetermined quantity of plasma from a blood sample for analysis purposes, the method making use of the flowing items:

a longitudinal transfer strip made of transparent plastic material and comprising a housing enclosed by a lid, said housing comprising a bottom, first and second opposing end faces, first and second opposing longitudinal side faces, and being compartmented so as to present;

along said first longitudinal side face and running sequentially from said first end face, a receptacle for receiving a blood sample connected to an outlet channel, a plasma storage cell and a red corpuscle storage cell opening into said outlet channel; and

parallel to said second longitudinal side face and starting from said first end face, a measurement cell connected by a capillary tube to a central plasma removal cell, having an outlet orifice through the bottom of said housing, and then to an overflow channel which opens into said plasma storage cell and then into an overflow tank, an end of the measurement cell opposite to said capillary tube being connected by a capillary duct to overflow cells;

and a turntable rotatable about its center and on which said transfer strip may be disposed radially in two positions A and B, the position A being one where said first end face is closer to the center of the turntable than said second end face, and the position B being one where the strip is symmetrical to that of position A and where the second end face is closer to the center of the turntable than the first end face;

said method comprising the steps in sequence of;

inserting a sample of a few millimeters of blood through an orifice provided in said lid of said transfer strip so that said blood drops into said receptacle;

disposing said transfer strip in said A position on said turntable and rotating said turntable to cause said sample of blood to pass into said plasma storage cell, said corpuscle storage cell, and said overflow tank, the rotating causing plasma to appear in said plasma storage cell and red corpuscles to appear in said corpuscle storage cell;

placing said transfer strip in the B position on said turntable, and rotating said turntable thereby causing said measurement cell to be filled with plasma from said plasma storage cell; and

placing said transfer strip in said A position on said turntable, and further rotating said turntable thereby emptying said measurement cell and causing a calibrated quantity of plasma to pass into said removal cell and thence out from said housing via said outlet orifice.

2. The method according to claim 1, wherein said housing further includes a longitudinal channel running along said second longitudinal side face between outlets of said overflow cells opposite to their connection with the measurement cell via the capillary duct and a plasma storage tank situated adjacent to said second end face, and wherein said method further comprises, after causing the calibrated quantity of plasma to pass into said removal cell and thence out from said housing via said outlet orifice:

placing said transfer strip in the B position and rotating said turntable in order to refill said measurement cell from said storage tank; and

again placing said transfer strip in said A position and again rotating said turntable in order to empty said measurement cell into said removal cell.

3. A transfer strip for supplying at least one predetermined quantity of plasma from a blood sample for analysis purposes by disposing said transfer strip radially on a turntable rotatable about its center and periodically rotating said transfer strip by 180°

into two positions A and B, the position A corresponding to a position where one end face is closer to the center of the turntable than the other end face and the position B being one where the transfer strip is symmetrical to that at position A with the other end face closer to the center of the turntable and said one end face further therefrom; and

rotating said turntable sequentially after changing the transfer strip alternately from position A to position B, said transfer strip comprising;

a housing, a lid closing said housing, both said lid and said housing being made of transparent plastic material, said housing comprising first and second longitudinal end faces, first and second longitudinal side faces and a bottom, and including compartmented means defining;

along said first longitudinal side face and running sequentially from said first end face, a receptacle for receiving a blood sample connected to an outlet channel, a plasma storage cell and a red corpuscle storage cell opening to said outlet channel; and

parallel to said second longitudinal side face and starting from said first end face, a measurement cell connected by a capillary tube to a central plasma removal cell, having an outlet orifice extending through said bottom of said housing, and then to an overflow channel which opens into said plasma storage cell and then into an overflow tank, and wherein an end of the measurement cell opposite to said capillary tube is connected by a capillary duct to overflow cells.

4. The transfer strip according to claim 3, wherein said housing further includes a longitudinal channel running along said second longitudinal side face between outlets of said overflow cells opposite to their connection with the measurement cell via the capillary duct and a plasma storage tank situated adjacent to said second end face.