Method and apparatus for nondestructive in vivo measurement of photosynthesis

• US 4,789,436 A
• Filed: 02/22/1988
• Issued: 12/06/1988
• Est. Priority Date: 02/22/1988
• Status: Expired due to Fees

• Current Assignee: The United States of America As Represented By The Secretary of Agriculture
• Original Assignee: United States Department of Energy (Government of the United States of America)
• Inventors: Greenbaum, Elias
• Primary Examiner(s): Kaplan, G. L.
• Application Number:
  US07/158,500
• Time in Patent Office: 288 Days
• Field of Search: 204/415, 204/1 P, 204/403, 435/29
• US Class Current: 205/778
• CPC Class Codes: G01N 27/404 Cells with anode, cathode and cell electrolyte on the same side of a permeable membrane which separates them from the sample fluid , e.g. Clark-type oxygen sensors
• CPC Class Codes: G01N 33/4977 Metabolic gas from microbes, cell cultures or plant tissues

Abstract

A device for in situ, nondestructive measurement of photosynthesis in live plants and photosynthetic microorganisms is disclosed which comprises a Clark-type oxygen electrode having a substantially transparent cathode comprised of an optical fiber having a metallic grid microetched onto its front face and sides, an anode, a substantially transparent electrolyte film, and a substantially transparent oxygen permeable membrane. The device is designed to be placed in direct contact with a photosynthetic portion of a living plant, and nondestructive, noninvasive measurement of photosynthetic oxygen production from the plant can be taken by passing light through the fiber-optic cathode, transparent electroyte and transparent membrane, and onto the plant so that photosynthesis occurs. The oxygen thus produced by the plant is measured polargraphically by the electrode. The present invention allows for rapid, nondestructive measurements of photosynthesis in living plants in a manner heretofore impossible using prior art methods.

Claims

1. A device for nondestructive measurement of photosynthesis in living organisms comprising:

a Clark-type oxygen electrode having a substantially transparent cathode comprised of an optically transparent member having a metallic grid on its sides and front face, an anode, a substantially transparent electrolyte film in contact with the front face of said cathode, and a substantially transparent oxygen permeable membrane in contact with said electrolyte film, so that the oxygen produced by the photosynthetic system of a living photosynthetic organism in response to light directed onto said organism from said cathode will diffuse through said membrane and said electrolyte film so as to be measurable polarographically by said Clark-type oxygen electrode.

2. A device according to claim 1 wherein said optically transparent member is comprised of a fiber-optic material.

3. A device according to claim 2 wherein said optically transparent member is a cylindrical optical fiber.

4. A device according to claim 1 wherein said metallic grid is comprised of platinum.

5. A device according to claim 1 wherein said metallic grid is comprised of gold.

6. A device according to claim 1 further comprising a retaining cup attached to said oxygen permeable membrane to allow measurement of photosynthesis in photosynthetic microorganisms.

7. A method of nondestructively measuring photosynthesis in living organisms which comprises the steps of:

(a) positioning a Clark-type oxygen electrode having a substantially transparent cathode comprised of an optically transparent member with a metallic grid on its sides and front face, a substantially transparent electrolyte film in contact with the cathode, and a substantially transparent oxygen permeable membrane next to a living photosynthetic organism so that oxygen produced by the organism in response to light will diffuse through the oxygen permeable membrane;

(b) causing light to be conducted through the optically transparent member of the cathode so that the light travels through the electrolyte film and oxygen permeable membrane and impinges upon the photosynthetic organism so that oxygen is produced by the organism; and

(c) measuring polargraphically the oxygen produced by the photosynthetic organism.