Process for producing bovine lactoferrin in high purity

• US 4,791,193 A
• Filed: 07/16/1987
• Issued: 12/13/1988
• Est. Priority Date: 07/17/1986
• Status: Expired due to Term

• Current Assignee: Morinaga Milk Industry Company Limited
• Original Assignee: Morinaga Milk Industry Company Limited
• Inventors: Tamura, Yoshitaka, Tomita, Mamoru, Mizota, Teruhiko, Okonogi, Shigeo, Tomimura, Toshio
• Primary Examiner(s): Schain, Howard E.
• Application Number:
  US07/074,034
• Time in Patent Office: 516 Days
• Field of Search: 530/366, 530/365, 530/837, 530/833, 424/85
• US Class Current: 530/416
• CPC Class Codes: A23J 1/20 from milk, e.g. casein curds or cheese A23C; from whey
• CPC Class Codes: Y10S 530/832 Milk; colostrum
• CPC Class Codes: Y10S 530/833 Whey; cheese

Abstract

A process for producing bovine lactoferrin in high purity form raw milk-materials containing skim milk or whey originated form cow'"'"'s milk which comprises: adsoption step wherein said raw materials are contacted, at a temperature between 0°-60° C., with weakly acidic cation-exchanger which includes carboxymethyl groups as ion exchanging groups and has haemoglobin adsorption property more than 3.5 g/100 ml; rinsing step wherein said exchanger is washed with water to remove substances other than those adsorbed to said exchanger; and desorption step wherein the substances adsorbed to said exchanger are desorbed therefrom with a solution of one or more of salts to thereby yield highly purified bovine lactoferrin without deterirating raw milk materials. Said desorption step can be carried out in a single stage to produce lactoferrin more than 80% purity to total proteins recovered, and in two stage to attain more than 98% purity.

Claims

1. A process for producing bovine lactoferrin in high purity from raw milk materials containing skim milk or whey originating from cow'"'"'s milk, which comprises:

3. The process as claimed in claim 1, wherein said cation exchange resin has a volume variation ratio equal to or less than 1.5, which is the value that is given by the bed volume of Na form of said exchange resin swelled with water in a column divided by the bed volume of the same resin equilibrated with sodium chloride solution having 0.5 ionic strength.

4. The process as claimed in claim 1, which further comprises a step before said desorbing step, wherein said cation exchange resin is washed with a first 0.4-2.5 wt.% solution of one or more salts selected from the group consisting of sodium chloride, potassium chloride, calcium chloride and magnesium chloride, to thereby remove contaminants, and said desorbing step is carried out with a second 1.5-12 wt.% solution of one or more of said salts, and wherein the concentration of said salt is higher in said second solution than said first solution.

5. The process as claimed in claim 1 wherein the purity of the yielded bovine lactoferring is equal to or more than 80% of the total proteins desorbed from said exchanger.

6. The process as claimed in claim 3 wherein the purity of the yielded lactoferrin is equal to or more than 98% of the total proteins desorbed from said exchanger.

2. (a) contacting said raw materials with a weakly acidic cation exchange resin which contains as cation exchange groups carboxymethyl groups, and has a hemoglobin adsorbing property more than 3.5 g/100 ml, at a temperature of between 0°

and 60°

C. to thereby adsorb said lactoferrin on said resin;

(b) washing said ion exchange resin with water to remove substances other than those adsorbed on said cation exchange resin; and

(c) desorbing said bovine lactoferrin from said cation exchange resin by washing said resin with a solution of at least one salt, to thereby yield purified bovine lactoferrin.