Implantable prosthetic device with lectin linked endothelial cells

• US 4,795,459 A
• Filed: 05/18/1987
• Issued: 01/03/1989
• Est. Priority Date: 05/18/1987
• Status: Expired due to Term

• Current Assignee: Rhode Island Hospital
• Original Assignee: Rhode Island Hospital
• Inventors: Jauregui, Hugo O.
• Primary Examiner(s): Apley, Richard J.
• Assistant Examiner(s): Prebilic, Paul B.
• Application Number:
  US07/051,500
• Time in Patent Office: 596 Days
• Field of Search: 128/DIG. 3, 435/1, 435/240, 435/180, 435/182, 436/827, 623/1, 623/2, 623/11, 623/12, 623/66
• US Class Current: 623/1.41
• CPC Class Codes: A61F 2/06 Blood vessels
• CPC Class Codes: A61F 2/062 Apparatus for the production of blood vessels made from natural tissue or with layers of living cells prostheses made from natural tissue or living cells A61L27/507
• CPC Class Codes: A61L 27/36 containing ingredients of undetermined constitution or reaction products thereof , e.g. transplant tissue, natural bone, extracellular matrix isolated natural compounds, e.g. collagen A61L27/24
• CPC Class Codes: A61L 27/507 for artificial blood vessels apparatus for applying cells on a blood vessel prosthesis A61F2/062
• CPC Class Codes: C12N 11/082 obtained by reactions only involving carbon-to-carbon unsaturated bonds
• CPC Class Codes: C12N 11/093 Polyurethanes
• CPC Class Codes: C12N 11/096 Polyesters; Polyamides
• CPC Class Codes: Y10S 436/827 Lectins

Abstract

An implantable prosthetic device made of biocompatible polymer and having a substantially continuous layer of autologus living cells attached via oligosaccharidelectin recognition linkages.

Claims

1. An implantable prosthetic device comprising

a member that has a shape to perform a cardiovascular prosthetic function when implanted, is made of biocompatible polymer, and has a surface to which a substantially continuous layer of autologous living endothelial cells has been attached via oligosaccharide-lectin recognition linkages.

7. The device of claim 1 wherein said member is a tubular vessel that is sufficiently small to function as a coronary artery.

8. The device of claim 1 wherein said polymer is PTFE.

9. The device of claim 1 wherein said polymer is polyurethane.

10. The device of claim 1 wherein said polymer is Dacron.

11. The device of claim 1 wherein said polymer is a bioresorbable polymer of a compound of the Krebs cycle.

2. The device of claim 2 wherein said lectin is covalently bound to said polymer, and said oligosaccharide is part of the cell surface of said living cells.

3. The device of claim 2 wherein said lectin is a lectin that recognizes α

-L-fucose.

4. The device of claim 2 wherein said lectin is one of the group consisting of Ulex Europaeus agglutinin I, Lens culinaris agglutinin, Pisum sativum agglutinin, wheat germ agglutinin, Lotus tetragonolobus agglutinin, Ricinus communis agglutinin, Concanavalin A, and Cytisus sessilifolius agglutinin.

5. The device of claim 2 wherein said lectin is Ulex Europaeus agglutinin I.

6. The device of claim 2 wherein said lectin is wheat germ agglutinin.