Well-type diagnostic plate device

• US 4,797,259 A
• Filed: 12/15/1986
• Issued: 01/10/1989
• Est. Priority Date: 12/15/1986
• Status: Expired due to Term

• Current Assignee: Pall Corporation (Danaher Corporation)
• Original Assignee: Pall Corporation (Danaher Corporation)
• Inventors: Martin, Jerold, Matkovich, Vlado I., Degen, Peter J.
• Primary Examiner(s): NOT, DEFINED
• Assistant Examiner(s): NOT, DEFINED
• Application Number:
  US06/941,386
• Time in Patent Office: 757 Days
• Field of Search: 422/101, 422/102, 435/7, 435/301, 356/246
• US Class Current: 422/534
• CPC Class Codes: B01D 61/18 Apparatus therefor
• CPC Class Codes: B01L 3/50255 Multi-well filtration
• CPC Class Codes: G01N 33/54366 Apparatus specially adapted for solid-phase testing

Abstract

A diagnostic test device is provided including a plate having at least one well, preferably a plurality of wells, each with an open bottom across which a composite membrane comprising three layers is placed, with a hydrophobic, liquid-tight seal provided at the periphery of each well. The composite membrane from the top of upstream side to the bottom or downstream side, in sequence, includes a first reaction or filtration layer formed from a thin, liquophilic, microporous membrane, a second or sealing layer, preferably a hydrophobic material in sheet or fiber form, such as nonwoven polypropylene fibers, and a liquophobic, preferably hydrophobic, barrier layer having one or more apertures which allows liquid to exit the well while eliminating lateral migration of a pendant liquid drop. The liquophobic seal provided by the liquophobic sealing layer eliminates "cross-talk" by lateral diffusion or wicking.

Claims

1. A diagnostic test device comprising:

a plate,

at least one well formed in said plate, said at least one well having an open bottom, and

a composite membrane having at least three layers including, sequentially, a microporous liquophilic reaction layer, a sealing layer, and a liquophobic barrier layer having at least one aperture therein for each said well and located substantially at the center of the well, said composite membrane coextensive with the bottom of said at least one well and forming a liquophobic, liquid-tight seal at the periphery of the well.

2. The diagnostic test device of claim 1 wherein said reaction layer comprises a hydrophilic material.

3. The diagnostic test device of claim 1 wherein said sealing layer comprises a synthetic, thermoplastic web of microfibers.

4. The diagnostic test device of claim 1 wherein said liquophobic, liquid-tight seal is a hydrophobic liquid-tight seal.

5. The diagnostic test device of claim 1 wherein said reaction layer includes a hole located at substantially the center of said well.

6. The diagnostic test device of claim 1 wherein said microporous reaction layer comprises a biologically inert polymeric material.

7. The diagnostic test device of claim 1 wherein said microporous reaction layer is formed from a polyamide or polyvinylidene difluoride.

8. The diagnostic test device of claim 1 further including a test reagent immobilized on said reaction layer.

9. The diagnostic test device of claim 1 wherein said barrier layer is formed from a material having a greater liquophobicity than that of either the reaction layer or the sealing layer.

10. The diagnostic test device of claim 1 wherein said at least one aperture consists of one aperture.

11. The diagnostic test device of claim 1 wherein said three layers are in intimate contact with one another.

12. The diagnostic test device of claim 11 wherein said three layers are bonded to one another.

13. The diagnostic test device of claim 1 wherein said at least one well comprises a plurality of wells.

14. The diagnostic test device of claim 13 wherein said composite membrane covers and is substantially coextensive with the bottoms of all of said wells.

15. The diagnostic test device of claim 1 wherein said device is adapted to be operatively associated with a differential pressure.

16. The diagnostic test device of claim 15 wherein said differential pressure is a reduced pressure.

17. The diagnostic test device of claim 1 wherein said microporous reaction layer comprises a hydrophilic, surface-modified polyamide.

18. The diagnostic test device of claim 17 wherein said surface-modified polyamide is a surface-modified nylon 66.

19. The diagnostic test device of claim 1 wherein said sealing layer comprises a hydrophobic material.

20. The diagnostic test device of claim 19 wherein said hydrophibic sealing layer is formed from a polyamide, a linear polyester, a polyolefin, a copolymer of two or more olefins, or mixtures of the aforementioned polymers.

21. The diagnostic test device of claim 20 wherein said hydrophobic sealing layer comprises polypropylene.

22. A diagnostic test system comprising:

(1) a diagnostic test device including;

(a) a plate,

(b) at least one well formed in said plate, said at least one well having an open bottom, and

(c) a composite membrane having at least three layers including, sequentially, a microporous liquophilic reaction layer, a sealing layer, and a liquophobic barrier layer having at least one aperture therein for each said well and located substantially at the center of the well, said composite membrane coextensive with the bottom of said at least one well and forming a hydrophobic, liquid-tight seal at the periphery of the well; and

(2) means for generating a differential pressure operatively associated with said diagnostic test device.

23. The diagnostic test system of claim 22 wherein said at least one aperture consists of one aperture.

24. The diagnostic test system of claim 22 wherein said differential pressure generating means comprises a vacuum manifold adapted to be operatively associated with a means for generating a reduced pressure.

25. The diagnostic test system of claim 24 wherein said vacuum manifold is in air-tight sealing relationship with said diagnostic test device.

26. The diagnostic test system of claim 24 wherein said vacuum manifold is formed integral with said diagnostic test device.

27. A diagnostic test device comprising:

(a) a first plate,

at least one well formed in said first plate, said at least one well having an open bottom, and

a composite membrane having at least three layers including, sequentially, a microporous, liquophilic membrane reaction layer, a sealing layer, and a liquophobic barrier layer having at least one aperture therein for each said well and located substantially at the center of the well, said composite membrane coextensive with the bottom of said at least one well of said first plate and forming a liquophobic liquid-tight seal at the periphery of the well; and

(b) a second plate in liquid receiving relationship with said first plate,

at least one well having an impermeable bottom in said second plate to receive liquid from said at least one well in said first plate.

28. The diagnostic test device of claim 27 wherein said at least one aperture consists of one aperture.

29. A diagnostic test system comprising:

(1) a diagnostic test device including;

(a) a first plate,

(b) at least one well formed in said first plate, said at least one well having an open bottom, and

(c) a composite membrane having at least three layers including, sequentially, a microporous, liquophilic membrane reaction layer, a sealing layer, and a liquophobic barrier layer having at least one aperture therein for each said well and located substantially at the center of the well, said composite membrane coextensive with the bottom of said at least one well of said first plate and forming a liquophobic liquid-tight seal at the periphery of the well;

(2) means for generating a differential pressure operatively associated with said diagnostic test device; and

(3) a second plate located intermediate and in operative relationship with said diagnostic test device and said differential pressure generating means, said second plate in liquid receiving relationship with said first plate,

at least one well having an impermeable bottom located in said second plate to receive liquid from said at least one well in said first plate.

30. The diagnostic test system of claim 29 wherein said at least one aperture consists of one aperture.

31. The diagnostic test system of claim 29 wherein said differential pressure generating means comprises a vacuum manifold adapted to be operatively associated with a means for generating a reduced pressure.

32. The diagnostic test system of claim 31 wherein said vacuum manifold is in air-tight sealing relationship with said diagnostic test device.

33. The diagnostic test system of claim 31 wherein said second plate is located within said vacuum manifold.

34. The diagnostic test system of claim 31 wherein said vacuum manifold is formed integral with said diagnostic test device.

35. The diagnostic test system of claim 34 wherein said second plate is located within said vacuum manifold.