Method for selection of antigens suitable as in vivo targets for antibodies
First Claim
1. A process for the selection of antigens suitable as targets for antibodies which localize in tumors in animals in vivo comprising:
- (a) preparing antibodies distinguishable from those present in the animal in which tumor targeting is to occur and that bind to antigens present in the tumor in said animal;
(b) injecting the prepared antibodies into a non-tumor-bearing animal and into a tumor-bearing animal to permit biofiltration of the antibodies;
(c) recovering the biofiltered antibodies from both the non-tumor-bearing animal and the tumor-bearing animal;
(d) identifying antigens whose antibodies are not retained in vivo in the non-tumor-bearing animal, but are retained in vivo in the tumor-bearing animal using the recovered biofiltered antibodies; and
(e) screening the antibodies that are not retained in vivo by the non-tumor-bearing animal and the antibodies that are not retained in vivo by the tumor-bearing animal to determine those antibodies that are actually retained in vivo in the tumor-bearing animal and to identify the antigens corresponding to those antibodies retained in the tumor-bearing animal.
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Accused Products
Abstract
The present invention is a process for the selection of antigens which are suitable targets for in vivo antibody localization in tumors or other altered (or diseased) tissue. The process provides a simplified and rapid technique for discovering useful in vivo targets for antibodies and is useful in cancer detection and therapy in humans or animals, whether or not the antigens are specific to tumors.
More specifically, the invention relates to a process for the selection of antigens suitable as targets for antibodies which localize in tumors in animals in vivo comprising preparing antibodies distinguishable from those present in the animal in which tumor targeting is to occur and that bind to antigens present in the tumor in said animal; injecting the prepared antibodies into a non-tumor-bearing animal to permit biofiltration of the antibodies; recovering the biofiltered antibodies from the non-tumor-bearing animal; employing the recovered biofiltered antibodies to identify antigens whose antibodies are not retained in vivo in the animal; and screening the antibodies that are not retained in vivo by the non-tumor bearing animal both to determine those antibodies that are actually retained in vivo in the tumor-bearing animal and to identify the antigens corresponding to those antibodies retained in the tumor-bearing animal.
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Citations
7 Claims
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1. A process for the selection of antigens suitable as targets for antibodies which localize in tumors in animals in vivo comprising:
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(a) preparing antibodies distinguishable from those present in the animal in which tumor targeting is to occur and that bind to antigens present in the tumor in said animal; (b) injecting the prepared antibodies into a non-tumor-bearing animal and into a tumor-bearing animal to permit biofiltration of the antibodies; (c) recovering the biofiltered antibodies from both the non-tumor-bearing animal and the tumor-bearing animal; (d) identifying antigens whose antibodies are not retained in vivo in the non-tumor-bearing animal, but are retained in vivo in the tumor-bearing animal using the recovered biofiltered antibodies; and (e) screening the antibodies that are not retained in vivo by the non-tumor-bearing animal and the antibodies that are not retained in vivo by the tumor-bearing animal to determine those antibodies that are actually retained in vivo in the tumor-bearing animal and to identify the antigens corresponding to those antibodies retained in the tumor-bearing animal. - View Dependent Claims (2, 3, 4, 5, 6, 7)
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Specification