Process for the microbiological preparation of aldose-1-epimerase

• US 4,800,161 A
• Filed: 09/05/1985
• Issued: 01/24/1989
• Est. Priority Date: 09/05/1984
• Status: Expired due to Term

• Current Assignee: Merck Patent GmbH (Merck & Co., Inc.)
• Original Assignee: Merck Patent GmbH (Merck & Co., Inc.)
• Inventors: Metz, Harald, Schmid, Gunter, Behrendt, Ulrich, Brummer, Wolfgang, Ebeling, Wolfgang
• Primary Examiner(s): Warden, Robert J.
• Assistant Examiner(s): Spiegel, Jack
• Application Number:
  US06/772,979
• Time in Patent Office: 1,237 Days
• Field of Search: 435/4, 435/14, 435/25, 435/26, 435/253, 435/822, 435/814
• US Class Current: 435/233
• CPC Class Codes: C12N 9/90 Isomerases (5.)
• CPC Class Codes: C12Q 1/54 involving glucose or galactose
• CPC Class Codes: Y10S 435/814 Enzyme separation or purification
• CPC Class Codes: Y10S 435/822 using bacteria or actinomycetales

Abstract

The invention relates to a process for the microbiological preparation of aldose-1-epimerase by cultivating microorganisms in a nutrient medium and release of the enzyme from the cells, which process is characterized in that microorganisms of the genus Acinetobacter are used.

Claims

1. A process for the microbiological preparation of aldose-1-epimerase comprising extracting and isolating said aldose-1-epimerase from a culture of a microorganism of the genus Acinetobacter.

2. A process for the microbiological preparation of aldose-1-epimerase, comprising cultivating a microorganism of the genus Acinetobacter in a nutrient medium, and subsequently releasing the aldose-1-epimerase from the resulting cells and isolating said aldose-1-epimerase.

3. A process according to claim 2, wherein the microorganism is Acinetobacter calcoaceticus.

4. A process according to claim 3, wherein the microorganism is a strain of Acinetobacter calcoaceticus DSM 30,007, DSM 30,008, DSM 30,010, DSM 30,011, ATCC 15 567, ATCC 23 055, ATCC 23 221 or ATCC 23 237.

5. A process according to claim 4, wherein the nutrient medium contains a carbon source which is lactic acid or citric acid.

6. A process according to claim 4, wherein the nutrient medium contains a carbon source which is glucose, fructose, sucrose, galactose or xylose.

7. A process according to claim 4, wherein the nutrient medium contains a carbon source which is glycerol, 2,3-butanediol or ethanol.