Technique for drug and chemical delivery

• US 4,801,459 A
• Filed: 08/05/1986
• Issued: 01/31/1989
• Est. Priority Date: 08/05/1986
• Status: Expired due to Fees

• Current Assignee: Robert P. Liburdy
• Original Assignee: Robert P. Liburdy
• Inventors: Liburdy, Robert P.
• Primary Examiner(s): Lovering, Richard D.
• Application Number:
  US06/893,436
• Time in Patent Office: 910 Days
• Field of Search: 264/4.3, 424/450, 436/829, 128/804
• US Class Current: 604/20
• CPC Class Codes: A61K 41/0028 Disruption, e.g. by heat or ultrasounds, sonophysical or sonochemical activation, e.g. thermosensitive or heat-sensitive liposomes, disruption of calculi with a medicinal preparation and ultrasounds
• CPC Class Codes: A61K 9/127 Liposomes
• CPC Class Codes: A61N 1/40 Applying electric fields by inductive or capacitive coupling microwave apparatus A61N5/00; Applying radio-frequency signals
• CPC Class Codes: A61N 5/02 using microwaves
• CPC Class Codes: Y10S 436/829 Liposomes, e.g. encapsulation
• CPC Class Codes: Y10T 428/2984 Microcapsule with fluid core [includes liposome]

Abstract

A technique for rapidly delivering drugs and/or chemicals to a specific area of the body by subjecting a liposome encapsulated drug and/or chemical to nonionizing electromagnetic fields. By subjecting the liposome to such fields in the range of 26-2,450 MHz drug/chemical release occurs in a rapid fashion and at temperatures that the liposomes are normally not leaky. This is a non-thermal effect of the field on the liposome that is not related to heating. Consequently, localized release of drug/chemicals can be effected without subjecting the diseased tissue to adverse heating. In addition, drug/chemical release due to electromagnetic-field treatment of liposomes is enhanced by serum and/or oxygen.

Claims

1. A method of delivering a chemical agent to a preselected area of a body, comprising the steps of:

(a) encapsulating said chemical agent within a liposome, said liposome having a predetermined phase transition temperature at which said liposome is capable of releasing said chemical agent;

(b) injecting said liposome into the blood stream of said body; and

(c) subjecting said preselected area of said body to nonionizing electromagnetic fields without heating said preselected area of said body to said predetermined phase transition temperature in order to release said chemical agent from said liposome by nonthermal means at a temperature below said predetermined phase transition temperature.

2. A method of delivering a chemical agent to a preselected area of a body as defined in claim 1 further comprising the step of ensuring that said liposome is impermeable prior to subjecting said liposome to said electromagnetic fields.

3. A method of delivering a chemical agent to a preselected area of a body as defined in claim 2 wherein said electromagnetic fields are in the range of 26-2450 MHz.

4. A method of delivering a chemical agent to a preselected area of a body as defined in claim 3 wherein said preselected area of said body is subjected to said electromagnetic fields for periods of time as short as one second and at a power of approximately 60 mW/gram of tissue at said preselected area of said body.

5. A method of delivering a chemical agent to a preselected area of a body as defined in claim 4 wherein said liposome is a unilamellar vesicle.

6. A method of delivering a chemical agent to a preselected area of a body as defined in claim 5 wherein said unilamellar vesicle is made up of 4:

1 weight combination of dipalmitoylphosphatidylcholine and dipalmitoylphosphatidylglycerol prepared in a physiological buffer.

7. A method of delivering a chemical agent to a preselected area of a body as defined in claim 1 wherein said release of said chemical agent is enhanced by injecting serum into said preselected area of said body.

8. A method of delivering a chemical agent to a preselected area of a body as defined in claim 1 wherein said release of said chemical agent is enhanced by introducing oxygen into said preselected area of said body.

9. A method of delivering a chemical agent to a preselected area of said body as defined in claim 8 wherein said release of said chemical agent is enhanced by injecting serum into said preselected area of said body.

10. A method of delivering a chemical agent to a preselected area of a body, comprising the steps of:

(a) encapsulating said chemical agent within a liposome, said liposome having a predetermined phase transition temperature at which said liposome is capable of releasing said chemical agent;

(b) injecting said liposome into a preselected tissue of said body; and

(c) subjecting said preselected tissue of said body to nonionizing electromagnetic fields without heating said tissue to said predetermined phase transition temperature in order to release said chemical agent from said liposome by nonthermal means at a temperature below said predetermined phase transition temperature.

11. A method of delivering a chemical agent to a preselected area of said body as defined in claim 10 further comprising the step of ensuring that said liposome is impermeable prior to subjecting said liposome to said electromagnetic fields.

12. A method of delivering a chemical agent to a preselected area of a body as defined in claim 11 wherein said electromagnetic fields are in the range of 26≧

2450 MHz.

13. A method of delivering a chemical agent to a preselected area of a body as defined in claim 12 wherein said preselected area of said body is subjected to said electromagnetic fields for periods of time as short as one second and at a power of approximately 60 mW/gram of tissue at said preselected area of said body.

14. A method of delivering a chemical agent to a preselected area of said body as defined in claim 13 wherein said liposome is a unilamellar vesicle.

15. A method of delivering a chemical agent to a preselected area of a body as defined in claim 14 wherein said unilamellar vesicle is made up of 4:

1 weight combination of dipalmitoylphosphatidylcholine and dipalmitoylphosphatidylglycerol prepared in a physiological buffer.

16. A method of delivering a chemical agent to a preselected area of said body as defined in claim 10 wherein said release of said chemical agent is enhanced by injecting serum into said preselected area of said body.

17. A method of delivering a chemical agent to a preselected area of said body as defined in claim 10 wherein said release of said chemical agent is enhanced by introducing oxygen into said preselected area of said body.

18. A method of delivering a chemical agent to a preselected area of said body as defined in claim 17 wherein said release of said chemical agent is enhanced by injecting serum into said preselected area of said body.