Fluorescent labels having a polysaccharide bound to polymeric particles

• US 4,801,504 A
• Filed: 09/24/1987
• Issued: 01/31/1989
• Est. Priority Date: 03/18/1985
• Status: Expired due to Term

• Current Assignee: Clinical Diagnostic Systems Inc.
• Original Assignee: Eastman Kodak Company
• Inventors: Danielson, Susan J., Burdick, Brent A.
• Primary Examiner(s): NOT, DEFINED
• Assistant Examiner(s): NOT, DEFINED
• Application Number:
  US07/100,513
• Time in Patent Office: 495 Days
• Field of Search: 428/403, 428/690, 436/529, 436/533, 436/530, 436/534, 436/546
• US Class Current: 428/403
• CPC Class Codes: G01N 33/525 Multi-layer analytical elements
• CPC Class Codes: G01N 33/533 with fluorescent label
• CPC Class Codes: G01N 33/54386 Analytical elements
• CPC Class Codes: G01N 33/582 with fluorescent label
• CPC Class Codes: G01N 33/585 with a particulate label, e.g. coloured latex
• CPC Class Codes: Y10S 436/80 Fluorescent dyes, e.g. rhodamine
• CPC Class Codes: Y10S 436/805 Optical property
• CPC Class Codes: Y10S 436/808 Automated or kit
• CPC Class Codes: Y10T 428/2991 Coated

Abstract

Fluorescent labels comprise a polysaccharide bound to a polymeric particle which contains a fluorescent rare earth chelate. These labels can be attached to any of a variety of physiologically reactive species to provide labeled species which have improved stability in aqueous solutions. The labeled species are particularly useful in specific binding assays to determine an immunologically reactive ligand, e.g. a hapten, in either solution or dry analytical techniques.

Claims

1. A fluorescent label comprising a polysaccharide bound to a polymeric particle which is derived from a loadable latex having a discontinuous phase which consists essentially of a polymer prepared from one or more ethylenically unsaturated polymerizable monomers, and an aqueous phase, said polymeric partilce having incorporated therein a fluorescent rare earth chelate.

2. The label of claim 1 wherein said polysaccharide is a dextran or lipopolysaccharide and has a molecular weight of from about 10⁴ to about 10⁷ daltons.

3. The label of claim 1 wherein the chelate is a chelate of europium or terbium and a 1,3-diketone, phthalate, naphthoate, dipyridine, terpyridine or phenanthroline chelating agent.

4. The label of claim 1 wherein said polymer comprises

(a) from 0 to 100 weight percent of units derived from a styrene or vinyl naphthyl monomer,

(b) from 0 to about 90 weight percent of units derived from an ethylenically unsaturated monomer of the structure ##STR5## wherein R is hydrogen or alkyl, R₁ is hydrogen or methyl, R₂ is hydrogen, halogen, alkyl, cyano, ##STR6## wherein R₃ is hydrogen or R₄, and R₄ is an aliphatic group, and

(c) from 0 to about 10 weight percent of units derived from an ethylenically unsaturated monomer containing one or more sulfonic acid or carboxy groups, or an ammonium or alkali metal salt thereof.