Method for detection of periodontal disease
First Claim
1. In a method for detection of aspartate aminotransferase in an oral fluid sample through (1 ) incubation of the sample with aspartate aminotransferase substrates in a first reaction mixture to form a first reaction product and (2) detection of reaction product therein by incubation with an indicator substance in a second reaction mixture wherein a second, colored reaction product is formed, the improvement comprising:
- (a) utilizing a diazonium compound as the indicator substance in the second reaction mixture; and
(b) modifying reaction conditions within the first and second reaction mixtures so that no visually detectable colored reaction product will be formed in the second reaction mixture when a selected subthreshold level of aspartate aminotransferase is present in said first reaction mixture but a visually detectable colored reaction product will be formed in the second reaction mixture when a selected nonzero suprathreshold concentration of aspartate aminotransferase is present in the first reaction mixture.
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Accused Products
Abstract
Disclosed are improvements in methods for determining the presence of suprathreshold amounts of aspartate aminotransferase in an oral fluid sample by (1) incubation of aspartate aminotransferase substrate materials in a first reaction mixture with the sample to form a first reaction product and (2) detection of reaction product therein by incubation with an indicator substance in a second reaction mixture wherein a second colored reaction product is formed. The improvement comprises (a) utilizing a diazonium dyestuff as the indicator substance in the second reaction and (b) modifying reaction conditions within the first and second reaction mixtures such that no visually detectable colored reaction product will be formed in the second reaction mixture by subthreshold level of aspartate aminotransferase but wherein a visually detectable color reaction product will be formed in the reaction mixture when a selected suprathreshold concentration of aspartate aminotransferase is present in the first reaction mixture.
17 Citations
15 Claims
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1. In a method for detection of aspartate aminotransferase in an oral fluid sample through (1 ) incubation of the sample with aspartate aminotransferase substrates in a first reaction mixture to form a first reaction product and (2) detection of reaction product therein by incubation with an indicator substance in a second reaction mixture wherein a second, colored reaction product is formed, the improvement comprising:
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(a) utilizing a diazonium compound as the indicator substance in the second reaction mixture; and (b) modifying reaction conditions within the first and second reaction mixtures so that no visually detectable colored reaction product will be formed in the second reaction mixture when a selected subthreshold level of aspartate aminotransferase is present in said first reaction mixture but a visually detectable colored reaction product will be formed in the second reaction mixture when a selected nonzero suprathreshold concentration of aspartate aminotransferase is present in the first reaction mixture. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15)
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Specification
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- Resources
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Current AssigneeColgate-Palmolive Company
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Original AssigneeXytronyx, Inc. (Eco-Shift Power Corp.)
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InventorsBaram, Peter, Babler, Scott D., Rudolph, Paul H., Waxman, Frank J.
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Primary Examiner(s)Nucker, Christine M.
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Application NumberUS06/840,890Time in Patent Office1,050 DaysField of Search435/15, 435/16, 435/24, 435/184, 435/810US Class Current435/16CPC Class CodesC12Q 1/52 involving transaminaseY10S 435/81 Packaged device or kit
