Method and composition for treating hypoglycemia using aloe polysaccharides

• US 4,801,582 A
• Filed: 06/27/1986
• Issued: 01/31/1989
• Est. Priority Date: 04/05/1984
• Status: Expired due to Fees

• Current Assignee: TOYO Yakushoku Kogyo Company Limited Araki-Cho 5-5 Shinjyuku-Ku Tokyo Japan
• Original Assignee: TOYO Yakushoku Kogyo Company Limited
• Inventors: Hayashi, Teruaki, Hikino, Hiroshi
• Primary Examiner(s): Griffin, Ronald W.
• Assistant Examiner(s): Carson, Nancy S.
• Application Number:
  US06/879,603
• Time in Patent Office: 949 Days
• Field of Search: 514/54, 514/866, 536/1.1, 536/123, 536/128
• US Class Current: 514/54
• CPC Class Codes: A61K 31/715 Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
• CPC Class Codes: A61K 36/886 Aloeaceae (Aloe family), e.g. aloe vera

Abstract

A hypoglycemic composition which contains as an effective component a polysaccharide named as Arboran A or B which has hypoglycemic activity and water solubility, and a method of treating diabetes comprising administering to a patient afflicted with diabetes a theraputically effective amount of the above composition.

Claims

1. A hypoglycemic composition comprising a pharmaceutically acceptable carrier and an active component selected from the group consisting of polysaccharides Arboran A, Arboran B and mixtures thereof, the polysaccharides having hypoglycemic activity and the following properties:

Arboran A

(a) solubility;

soluble in water and insoluble in benzene, ether, chloroform, alcohols and acetone;

(b) color reaction;

developing a pale yellowish red color on contact with a mixture of 2% aqueous solution of phenol and concentrated sulfuric acid, and being positive for silver mirror reaction and Fehling'"'"'s solution;

(c) specific rotation;

[α

]_D -37.7°

C 0.13, water);

(d) elemental analysis;

C, 41.11;

H, 5.87;

N, 0.43%;

(e) infrared absorption spectrum (KBr method) ν

max cm^-1 ;

3370, 1726, 1231, 1049, 1027;

(f) NMR spectrum;

¹ H-NMR δ

;

2.04, 2.12, 3.72, 4.96, 5.10, 5.43;

¹³ C-NMR δ

;

20.2, 20.4, 60.5, 70.0, 76.6, 172.8, 173.4;

(g) molecular weight (standard material;

dextran, Sephacryl S-200 gel filtration);

1.2×

10⁴ ;

(h) glass-fiber paper electrophoresis (borate buffer, pH 9.3);

moving distance;

17.1 cm (glucose, 10.9 cm);

(i) polyacrylamide-gel electrophoresis (10% polyacrylamide gel;

borate buffer, pH 9.3);

moving distance;

1.7 cm (bromophenol blue, 8.0 cm);

(j) neutral sugar content;

4. A method of treating diabetes which comprises administering to a patient afflicted with diabetes a theraputically effective amount of the composition according to claim 1.

2. 5% (Phenol --H₂ SO₄ method)

35.2% (Anthrone --H₂ SO₄ method)

50.5% (Cromotropic acid --H₂ SO₄ method);

(k) uronic acid content (Carbazol --H₂ SO₄ method);

5.0%;

(l) peptide content (Lowry method);

2.5%;

(m) O-acetyl content;

16.7%;

(n) neutral sugar components;

rhamnose-fructose-arabinose-xylose-mannose-galactose-glucose (0.3;

0.2;

0.1;

0.1;

0.2;

1.0;

0.3); and

Arboran B

(a) solubility;

soluble in water and insoluble in benzene, ether, chloroform, alcohols and acetone;

(b) color reaction;

developing a pale yellowish red color on contact with a mixture of 2% aqueous solution of phenol and concentrated sulfuric acid, and being positive for silver reaction and Fehling'"'"'s solution;

(c) specific rotation;

[α

]_D +162°

(C 0.12, water);

(d) elemental analysis;

C, 42.35;

H, 5.73;

N, 0.26%;

(e) infrared absorption spectrum (KBr method) ν

max cm^-1 ;

3350, 1052;

(f) NMR spectrum;

¹ H-NMR δ

;

1.21, 3.71, 5.03, 5.20;

¹³ C-NMR δ

;

61.4, 68.7, 70.0, 76.6, 81.0, 81.4, 84.1, 107.5;

(g) molecular weight;

(standard material;

dextran, Sephacryl S-200 gel filtration);

5.2×

10⁴ ;

(h) glass-fiber paper electrophoresis (borate buffer, pH 9.3);

moving distance;

15.6 cm (glucose 10.9 cm);

(i) polyacrylamide-gel electrophoresis (10% polyacrylamide-gel;

borate buffer, pH 9.3);

moving distance;

2.7 cm (bromophenol blue, 8.0 cm);

(j) neutral sugar content;

3. 4% (Phenol --H₂ SO₄ method)

82.0% (Anthrone --H₂ SO₄ method)

80.0% (Chromotropic acid --H₂ SO₄ method);

(k) uronic acid content (Carbazol --H₂ SO₄ method);

11.5%;

(l) peptide content (Lowry method);

10.4%;

(m) O-acetyl content;

5.3%;

(n) neutral sugar components;

Mannose-glucose (0.3;

1.0).