Method and composition for treating hypoglycemia using aloe polysaccharides
First Claim
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1. A hypoglycemic composition comprising a pharmaceutically acceptable carrier and an active component selected from the group consisting of polysaccharides Arboran A, Arboran B and mixtures thereof, the polysaccharides having hypoglycemic activity and the following properties:
- Arboran A(a) solubility;
soluble in water and insoluble in benzene, ether, chloroform, alcohols and acetone;
(b) color reaction;
developing a pale yellowish red color on contact with a mixture of 2% aqueous solution of phenol and concentrated sulfuric acid, and being positive for silver mirror reaction and Fehling'"'"'s solution;
(c) specific rotation;
[α
]D -37.7°
C 0.13, water);
(d) elemental analysis;
C, 41.11;
H, 5.87;
N, 0.43%;
(e) infrared absorption spectrum (KBr method) ν
max cm-1 ;
3370, 1726, 1231, 1049, 1027;
(f) NMR spectrum;
1 H-NMR δ
;
2.04, 2.12, 3.72, 4.96, 5.10, 5.43;
13 C-NMR δ
;
20.2, 20.4, 60.5, 70.0, 76.6, 172.8, 173.4;
(g) molecular weight (standard material;
dextran, Sephacryl S-200 gel filtration);
1.2×
104 ;
(h) glass-fiber paper electrophoresis (borate buffer, pH 9.3);
moving distance;
17.1 cm (glucose, 10.9 cm);
(i) polyacrylamide-gel electrophoresis (10% polyacrylamide gel;
borate buffer, pH 9.3);
moving distance;
1.7 cm (bromophenol blue, 8.0 cm);
(j) neutral sugar content;
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Abstract
A hypoglycemic composition which contains as an effective component a polysaccharide named as Arboran A or B which has hypoglycemic activity and water solubility, and a method of treating diabetes comprising administering to a patient afflicted with diabetes a theraputically effective amount of the above composition.
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Citations
4 Claims
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1. A hypoglycemic composition comprising a pharmaceutically acceptable carrier and an active component selected from the group consisting of polysaccharides Arboran A, Arboran B and mixtures thereof, the polysaccharides having hypoglycemic activity and the following properties:
Arboran A (a) solubility;
soluble in water and insoluble in benzene, ether, chloroform, alcohols and acetone;(b) color reaction;
developing a pale yellowish red color on contact with a mixture of 2% aqueous solution of phenol and concentrated sulfuric acid, and being positive for silver mirror reaction and Fehling'"'"'s solution;(c) specific rotation;
[α
]D -37.7°
C 0.13, water);(d) elemental analysis;
C, 41.11;
H, 5.87;
N, 0.43%;(e) infrared absorption spectrum (KBr method) ν
max cm-1 ;
3370, 1726, 1231, 1049, 1027;(f) NMR spectrum;
1 H-NMR δ
;
2.04, 2.12, 3.72, 4.96, 5.10, 5.43;
13 C-NMR δ
;
20.2, 20.4, 60.5, 70.0, 76.6, 172.8, 173.4;(g) molecular weight (standard material;
dextran, Sephacryl S-200 gel filtration);
1.2×
104 ;(h) glass-fiber paper electrophoresis (borate buffer, pH 9.3);
moving distance;
17.1 cm (glucose, 10.9 cm);(i) polyacrylamide-gel electrophoresis (10% polyacrylamide gel;
borate buffer, pH 9.3);
moving distance;
1.7 cm (bromophenol blue, 8.0 cm);(j) neutral sugar content; - View Dependent Claims (4)
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2. 5% (Phenol --H2 SO4 method)
35.2% (Anthrone --H2 SO4 method) 50.5% (Cromotropic acid --H2 SO4 method); -
(k) uronic acid content (Carbazol --H2 SO4 method);
5.0%;(l) peptide content (Lowry method);
2.5%;(m) O-acetyl content;
16.7%;(n) neutral sugar components;
rhamnose-fructose-arabinose-xylose-mannose-galactose-glucose (0.3;
0.2;
0.1;
0.1;
0.2;
1.0;
0.3); andArboran B (a) solubility;
soluble in water and insoluble in benzene, ether, chloroform, alcohols and acetone;(b) color reaction;
developing a pale yellowish red color on contact with a mixture of 2% aqueous solution of phenol and concentrated sulfuric acid, and being positive for silver reaction and Fehling'"'"'s solution;(c) specific rotation;
[α
]D +162°
(C 0.12, water);(d) elemental analysis;
C, 42.35;
H, 5.73;
N, 0.26%;(e) infrared absorption spectrum (KBr method) ν
max cm-1 ;
3350, 1052;(f) NMR spectrum;
1 H-NMR δ
;
1.21, 3.71, 5.03, 5.20;
13 C-NMR δ
;
61.4, 68.7, 70.0, 76.6, 81.0, 81.4, 84.1, 107.5;(g) molecular weight;
(standard material;
dextran, Sephacryl S-200 gel filtration);
5.2×
104 ;(h) glass-fiber paper electrophoresis (borate buffer, pH 9.3);
moving distance;
15.6 cm (glucose 10.9 cm);(i) polyacrylamide-gel electrophoresis (10% polyacrylamide-gel;
borate buffer, pH 9.3);
moving distance;
2.7 cm (bromophenol blue, 8.0 cm);(j) neutral sugar content;
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3. 4% (Phenol --H2 SO4 method)
82.0% (Anthrone --H2 SO4 method) 80.0% (Chromotropic acid --H2 SO4 method); -
(k) uronic acid content (Carbazol --H2 SO4 method);
11.5%;(l) peptide content (Lowry method);
10.4%;(m) O-acetyl content;
5.3%;(n) neutral sugar components;
Mannose-glucose (0.3;
1.0).
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Specification