Analytical element and method for determination of total lacate dehydrogenase
First Claim
1. A dry analytical element for the determination of total lactate dehydrogenase (LDH) comprising a support having thereona porous spreading layer composed of a particulate structure comprising a plurality of particles being bonded to each other on surface areas of said particles which are adjacent, where said adjacent particles form a coherent, three dimensional lattice which is essentially non-swellable to an aqueous liquid,said element further comprising a saturated or unsaturated fluorinated surfactant containing one or more fluorocarbon moieties each having from 4 to 16 carbon atoms and from 8 to 33 fluorine atoms, and each of said moieties having a ratio of fluorine atoms to carbon atoms of about 2:
- 1, provided that when said surfactant contains more than one such moiety, said moieties have up to two fluorinated or unfluorinated methyl branches.
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Accused Products
Abstract
A multilayer analytical element exhibits improved precision in the assay of total lactate dehydrogenase (LDH). The element also provides reduced susceptibility to total protein and hemoglobin interferences in the assay. The element contains a porous spreading layer composed of a particulate structure. A fluorinated surfactant is also used in the assay. This surfactant has one or more fluorocarbon moieties provided, that when the surfactant has more than one such moiety, they are substantially linear. The surfactant can be incorporated in the element or added at the time of the assay.
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Citations
19 Claims
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1. A dry analytical element for the determination of total lactate dehydrogenase (LDH) comprising a support having thereon
a porous spreading layer composed of a particulate structure comprising a plurality of particles being bonded to each other on surface areas of said particles which are adjacent, where said adjacent particles form a coherent, three dimensional lattice which is essentially non-swellable to an aqueous liquid, said element further comprising a saturated or unsaturated fluorinated surfactant containing one or more fluorocarbon moieties each having from 4 to 16 carbon atoms and from 8 to 33 fluorine atoms, and each of said moieties having a ratio of fluorine atoms to carbon atoms of about 2: - 1, provided that when said surfactant contains more than one such moiety, said moieties have up to two fluorinated or unfluorinated methyl branches.
- View Dependent Claims (2, 3, 4, 5, 6)
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7. A dry multilayer analytical element for the determination of total lactate dehydrogenase (LDH), said element comprising a support having thereon, in order from said support and in fluid contact,
a registration layer comprising an indicator material which provides a detectable spectrophotometric change in response to the reaction of LDH with a substrate for LDH, and an isotropically porous spreading layer comprising said LDH substrate and composed of a particulate structure comprising: -
(i) a plurality of heat-stable, organo-polymeric particles non-swellable in and impermeable to an aqueous liquid, and having a particle size of from about 1 to about 200 μ
m, and(ii) an adhesive, in an amount of up to about 10 weight percent of said particles, comprising an organic polymer different from that of said particles, substantially all of said adhesive being concentrated on surface areas of adjacent particles where said adjacent particles are in closest proximity, and bonding said particles into a coherent, three-dimensional lattice which is essentially non-swellable in said liquid, said element containing, in any of said layers, a saturated or unsaturated fluorinated surfactant containing one or more fluorocarbon moieties each having from 4 to 16 carbon atoms and from 8 to 33 fluorine atoms, and each of said moieties having a ratio of fluorine atoms to carbon atoms of about 2;
1, provided that when said surfactant contains more than one such moiety, said moieties have up to two fluorinated or unfluorinated methyl branches, which surfactant is present in a molar ratio to said substrate of from about 0.01;
1 to about 10;
1. - View Dependent Claims (8, 9, 10, 11)
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12. A method for the determination of total lactate dehydrogenase (LDH) comprising the steps of:
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A. in the presence of a substrate for LDH, an indicator material which provides a detectable change in response to the reaction of LDH with said substrate, and a saturated or unsaturated fluorinated surfactant containing one or more fluorocarbon moieties each having from 4 to 16 carbon atoms and from 8 to 33 fluorine atoms, and each of said moieties having a ratio of fluorine atoms to carbon atoms of about 2;
1,provided that when said fluorinated surfactant contains more than one fluorocarbon moiety, said moieties have up to two fluorinated or unfluorinated methyl branches, contacting an analytical element with a sample of a liquid suspected of containing LDH, said element comprising a support having thereon a porous spreading layer composed of a particulate structure comprising a plurality of particles being bonded to each other on surface areas of adjacent particles where the adjacent particles are in closest proximity to form a coherent, three dimensional lattice which is essentially non-swellable in an aqueous liquid, and B. determining total lactate dehydrogenase by detecting the rate of said detectable change.
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13. A metod for the determination of total lactate dehydrogenase (LDH) comprising the steps of:
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A. in the presence of a substrate for LDH and an indicator material which provides a detectable change in response to the reaction of LDH with said substrate, contacting a dry analytical element with a sample of a liquid suspected of containing LDH, said element comprising a support having thereon a porous spreading layer composed of a particulate structure comprising a plurality of particles being bonded to each other on surface areas of said particles which are adjacent, where said adjacent particles form a coherent, three dimensional lattice which is essentially non-swellable in an aqueous liquid, said element further comprising a saturated or unsaturated fluorinated surfactant containing one or more fluorocarbon moieties each having from 4 to 16 carbon atoms and from 8 to 33 fluorine atoms, and each of said moieties having a ratio of fluorine atoms to carbon atoms of about 2;
1, provided that when said surfactant contains more than one such moiety, said moieties have up to two fluorinated or unfluorinated methyl branches, andB. determining total lactate dehydrogenase by detecting the rate of said detectable change. - View Dependent Claims (14, 15, 16)
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17. A method for the determination of total lactate dehydrogenase (LDH) comprising the steps of:
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A. contacting a dry multilayer analytical element with a sample of a liquid suspected of containing LDH to provide a detectable colorimetric change, said element comprising a support having thereon, in order from said support and in fluid contact, a registration layer comprising an indicator material which provides a detectable spectrophotometric change in response to the reaction of LDH with a substrate for LDH, and an isotropically porous spreading layer comprising said LDH substrate and composed of a particulate structure comprising; (i) a plurality of heat-stable, organo-polymeric particles non-swellable in and impermeable to an aqueous liquid, and having a particle size of from about 1 to about 200 μ
m, and(ii) an adhesive, in an amount of up to about 10 weight percent of said particles, comprising an organic polymer different from that of said particles, substantially all of said adhesive being concentrated on surface areas of adjacent particles where said adjacent particles are in closest proximity, and bonding said particles into a coherent, three-dimensional lattice which is essentially non-swellable in said liquid, said element containing, in any of said layers, a saturated or unsaturated fluorinated surfactant containing one or more fluorocarbon moieties each having from 4 to 16 carbon atoms and from 8 to 33 fluorine atoms, and each of said moieties having a ratio of fluorine atoms to carbon atoms of about 2;
1, provided that when said surfactant contains more than one such moiety, said moieties have up to two fluorinated or unfluorinated methyl groups, which surfactant is present in a molar ratio to said substrate of from about 0.01;
1 to about 10;
1, andB. determining total lactate dehydrogenase by detecting the rate of said colorimetric change. - View Dependent Claims (18, 19)
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Specification